Effect of rhizobacteria treatments on nutrient content and organic and amino acid composition in raspberry plants

Plant growth-promoting rhizobacteria (PGPR) have been found to be beneficial to plant growth, yield, crop quality, theenvironment, and sustainable agricultural production. Therefore, six bacterial strains were tested to determine their effects on raspberry’snutrient content and organic and amino acid composition. The experiment was performed from 2015 to 2017. Two-year-old raspberryplants were inoculated with bacterial suspensions by a dipping method and were planted in 30-L pots. The mineral content and organicacid and amino acid composition of the leaf and root were compared in the Alcaligenes 637Ca, Staphylococcus MFDCa1 and MFDCa2,Agrobacterium A18, Pantoea FF1, and Bacillus M3 bacterial strains. Nitrogen (N) content of the leaf was 2.55% in the A18 treatment,while N content of the root was 1.61% in MFDCa2. The leaf ’s iron (Fe) content was highest in the M3 treatment with 91.76 mg kg–1,while 637Ca gave the highest root’s Fe content with 107.80 mg kg–1. The content of malonic acid (16.78 ng µL–1), malic acid (4.59 ngµL–1), citric acid (16.88 ng µL–1), and fumaric acid (4.94 ng µL–1) in leaves was higher in MFDCa2 than in the other treatments. Inaddition, 637Ca treatment had the highest root organic acid content in tartaric acid (5.94 ng µL–1), butyric acid (15.19 ng µL–1), andmaleic acid (5.13 ng µL–1). FF1 treatment was more effective than the other treatments for increasing the leaf ’s amino acid content,while the 637Ca, MFDCa1 FF1, and M3 treatments were more effective in increasing the root’s amino acid content. As a result, it wasdetermined that PGPR treatments play a significant role in mineral nutrient uptake and the organic acid and amino acid compositionof the raspberry plant.

PDF

___

Antoine FR, Wei CI, Littell RC, Marshall MR (1999). HPLC method for analysis of free amino acids in fish using o-phthaldialdehyde precolumn derivatization. J Agric Food Chem 47: 5100-5107.
Arıkan Ş, Eşitken A, İpek M, Aras S, Şahin M, Pırla, L, Turan M (2018). Effect of Plant Growth Promoting Rhizobacteria on Fe Acquisition in Peach (Prunus Persica L.) Under Calcareous Soil Conditions. Journal of Plant Nutrition, 41(17), 2141-2150.
Arikan Ş, Pirlak L (2016). Effects of plant growth promoting rhizobacteria (PGPR) on growth, yield and fruit quality of sour cherry (Prunus cerasus L.). Erwerbs-Obstbau 58: 221-226.
Aristoy MC, Toldra F (1991). Deproteinization techniques for HPLC amino acid analysis in fresh pork muscle and dry-cured ham. J Agric Food Chem 39: 1792-1795.
Aslantaş R, Çakmakçi R, Şahin F (2007). Effect of plant growth promoting rhizobacteria on young apple tree growth and fruit yield under orchard conditions. Sci Hortic-Amsterdam 111: 371-377.
Belimov A, Dodd I, Safronova V, Shaposhnikov A, Azarova T, Makarova N, Davies W, Tikhonovich I (2015). Rhizobacteria that produce auxins and contain 1-amino-cyclopropane-1- carboxylic acid deaminase decrease amino acid concentrations in the rhizosphere and improve growth and yield of wellwatered and water-limited potato (Solanum tuberosum). Ann Appl Biol 167: 11-25.
Bhattacharyya PN, Jha DK (2012). Plant growth-promoting rhizobacteria (PGPR): emergence in agriculture. World J Microb Biot 28: 1327-1350.
Bollard E (1960). Transport in the xylem. Annu Rev Plant Physiol 11: 141-166.
Bremner JM, Mulvaney C (1982). Nitrogen total. Methods of soil analysis. Part 2. Chemical and microbiological properties (methodsofsoilan2): 595-624.
Çakmakçı R, Dönmez MF, Ertürk Y, Erat M, Haznedar A, Sekban R (2010). Diversity and metabolic potential of culturable bacteria from the rhizosphere of Turkish tea grown in acidic soils. Plant Soil 332: 299-318.
Çakmakçı R, Kantar F, Sahin F (2001). Effect of N2 -fixing bacterial inoculations on yield of sugar beet and barley. J Plant Nutr Soil Sc 164: 527-531.
Ekinci M, Turan M, Yildirim E, Güneş A, Kotan R, Dursun A (2014). Effect of plant growth promoting rhizobacteria on growth, nutrient, organic acid, amino acid and hormone content of cauliflower (Brassica oleracea. var. botrytis) transplants. Acta Sci Pol-Hortoru 13: 71-85.
Elkoca E, Kantar F, Sahin F (2007). Influence of nitrogen-fixing and phosphorus solubilizing bacteria on the nodulation, plant growth, and yield of chickpea. J Plant Nutr 31: 157-171.
Erdogan U, Cakmakci R, Varmazyarı A, Turan M, Erdogan Y, Kıtır N (2016). Role of inoculation with multi-trait rhizobacteria on strawberries under water deficit stress. Zemdirbyste 103: 67-76.
Esitken A, Karlidag H, Ercisli S, Turan M, Sahin F (2003). The effect of spraying a growth promoting bacterium on the yield, growth and nutrient element composition of leaves of apricot (Prunus armeniaca L. cv. Hacihaliloglu). Crop Pasture Sci 54: 377-380.
Esitken A, Pirlak L, Turan M, Sahin F (2006). Effects of floral and foliar application of plant growth promoting rhizobacteria (PGPR) on yield, growth and nutrition of sweet cherry. Sci Hortic-Amsterdam 110: 324-327.
Esitken A, Yildiz HE, Ercisli S, Donmez MF, Turan M, Gunes A (2010). Effects of plant growth promoting bacteria (PGPB) on yield, growth and nutrient contents of organically grown strawberry. Sci Hortic-Amsterdam 124: 62-66.
Food and Agriculture Organization of the United Nations (FAO) (2017). The statistics of raspberry production. Henderson J, Ricker R, Bidlingmeyer B, Woodward C (1999). Amino acid analysis using Zorbax Eclipse-AAA Columns and the Agilent 1200 HPLC. Agilent Technologies Inc, Publication Number 5980-1193E.
İpek M, Aras S, Arıkan Ş, Eşitken A, Pırlak L, Dönmez MF, Turan M (2017a). Root plant growth promoting rhizobacteria inoculations increase ferric chelate reductase (FC-R) activity and Fe nutrition in pear under calcareous soil conditions. Sci Hortic-Amsterdam 219: 144-151.
İpek M, Arikan Ş, Pirlak L, Eşitken A (2017b). Effect of different treatments on branching of some apple trees in nursery. Erwerbs-Obstbau: 1-4.
Ipek M, Pirlak L, Esitken A, Dönmez MF, Turan M, Sahin F (2014). Plant growth-promoting rhizobacteria (PGPR) increase yield, growth and nutrition of strawberry under high-calcareous soil conditions. J Plant Nutr 37: 990-1001.
Jennings DL (1988). Raspberries and blackberries: their breeding, diseases and growth, London, UK: Academic Press. Jiménez-Gómez A, Celador-Lera L, Fradejas-Bayón M, Rivas R (2017). Plant probiotic bacteria enhance the quality of fruit and horticultural crops. Aims Microbiology 3: 483-501.
Karakurt H, Aslantas R (2010). Effects of some plant growth promoting rhizobacteria (PGPR) strains on plant growth and leaf nutrient content of apple. J Fruit Ornam Plant Res 18: 101- 110.
Köse M (2003). Selva ve Sweet Charlie çilek çeşitlerinde bakteri uygulamalarının bitki gelişimi ve verimi üzerine etkisi. Yüksek Lisans Tezi, Atatürk University, Erzurum, Turkey. Kudoyarova GR, Melentiev AI, Martynenko EV, Timergalina LN, Arkhipova TN, Shendel GV, Kuz’mina LY, Dodd IC, Veselov SY (2014). Cytokinin producing bacteria stimulate amino acid deposition by wheat roots. Plant Physiol Bioch 83: 285-291.
Lewis OAM (1991). Plants and Nitrogen. Cambridge, UK: Cambridge University Press.
Marschner H (2011). Marschner’s Mineral Nutrition of Higher Plants. 3rd ed. San Diego, CA, USA: Academic Press. Mertens D (2005). AOAC official method 975.03. Metal in Plants and Pet Foods. Official Methods of Analysis, 18th edn. Horwitz W, GW Latimer GW, editors. 3-4.
O’Connell PF (1992). Sustainable agriculture - a valid alternative. Outlook Agric 21: 5-12.
Ohwaki Y, Hirata H (1992). Differences in carboxylic acid exudation among P-starved leguminous crops in relation to carboxylic acid contents in plant tissues and phospholipid level in roots. Soil Sci Plant Nutr 38: 235-243.
Orhan E, Esitken A, Ercisli S, Turan M, Sahin F (2006). Effects of plant growth promoting rhizobacteria (PGPR) on yield, growth and nutrient contents in organically growing raspberry. Sci Hortic-Amsterdam 111: 38-43.
Ozturk A, Caglar O, Sahin F (2003). Yield response of wheat and barley to inoculation of plant growth promoting rhizobacteria at various levels of nitrogen fertilization. J Plant Nutr Soil Sc 166: 262-266.
Pırlak L, Köse M (2009). Effects of plant growth promoting rhizobacteria on yield and some fruit properties of strawberry. J Plant Nutr 32: 1173-1184.
Seymen M, Türkmen Ö, Dursun A, Paksoy M (2015a). Effects of bacteria inoculation on yield, yield components and mineral contents of tomato. Selçuk Tarım ve Gıda Bilimleri Dergisi 28: 52-57.
Seymen M, Türkmen Ö, Paksoy M (2015b). Bacteria inoculation effects on yield, yield components and mineral contents of (Capsicum annum L.) bell pepper. International Journal of Agriculture and Economic Development 3: 29-36.
Sudhakar P, Chattopadhyay GN, Gangwar SK, Ghosh JK (2000). Effect of foliar application of Azotobacter, Azospirillum and Beijerinckia on leaf yield and quality of mulberry (Morus alba). The Journal of Agricultural Science 134: 227-234.
Vessey JK (2003). Plant growth promoting rhizobacteria as biofertilizers. Plant Soil 255: 571-586.
Zimmermann MH (1960). Transport in the phloem. Annu Rev Plant Physiol 11: 167-190.