Seyed Ahmad Kalantar AHMADI, Ali EBADI, Sodabeh JAHANBAKHSH, Jahanfar DANESHIAN, Seyed Ataolah SIADAT

Changes in enzymatic and nonenzymatic antioxidant defense mechanisms of canola seedlings at different drought stress and nitrogen levels

To evaluate the responses of canola seedlings to different levels of drought stress and nitrogen at different growth stages, a factorial experiment was conducted in a completely randomized design with three replications at the experimental greenhouse of Mohaghegh Ardabili University in 2013. Treatments included three levels of drought stress (30%, 50%, and 70% of FC) and five levels of nitrogen (control, based on soil test results; 25% less than the recommended level; 50% less than the recommended level; 25% more than the recommended level; and 50% more than the recommended level). The recommended level of nitrogen, based on soil test results, was 0.09 g of nitrogen per kilogram of soil. Results showed that drought stress, nitrogen, and their interaction significantly affected the enzymatic activity of antioxidant catalase (CAT), polyphenol oxidase (PPO), and peroxidase (PO) at various stages of growth. Proline was also affected by drought stress and nitrogen. Maximum CAT activity (794.04 OD mg protein min-1) was observed at the 4-6 leaf stage under mild drought stress conditions (50% of FC) and a nitrogen application rate 50% less than the recommended level. Increased rates of nitrogen enhanced the PPO activity at the 4-6 and 6-8 leaf stages. PO had a negative response to increased rates of nitrogen application. The highest rate of increase in proline was at the 8-10 leaf stage: 205% and 207% higher under mild drought stress (50% of FC) and severe drought stress (30% of FC) conditions, respectively, compared to favorable moisture conditions (70% of FC). Increased nitrogen application led to an increase in proline production at all stages of sampling (4-6, 6-8, and 8-10 leaf stages).

Anahtar Kelimeler:

Catalase, peroxidase, proline, water deficit

Changes in enzymatic and nonenzymatic antioxidant defense mechanisms of canola seedlings at different drought stress and nitrogen levels

Keywords:

Catalase, peroxidase, proline, water deficit,

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Ain-Lhout F, Zunzunegui M, Diaz-Barradas MC, Tirado R, Clavijo A, Garcia-Novo F (2001). Comparison of proline accumulation in two Mediterranean shrubs subjected to natural and experimental water deficit. Plant and Soil 230: 175–183.
Aires A, Rosa E, Carvalho R (2006). Effect of nitrogen and sulfur fertilization on glucosinolates in the leaves and roots of broccoli sprouts (Brassica oleraceavar). J Sci Food Agr 86: 1512–1516.
Arora A, Sairam RK, Srivastava GC (2002). Oxidative stress and antioxidant system in plants. Current Science-Bangalore 82: 1227–1238.
Ashraf M, Foolad MR (2007). Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environ Exp Bot 59: 206–216.
Bates LS, Waldren RP, Teare ID (1973). Rapid determination of free proline for water stress studies. Plant and Soil 39: 205–207.
Boutraa T, Sanders FE (2001). Effects of interactions of moisture regime and nutrient addition on nodulation and carbon partitioning in two cultivars of bean (Phaseolus vulgaris L.). J Agron Crop Sci 186: 229–237.
Dhanda SS, Sethi SS, Behl RK (2004). Indices of drought tolerance in wheat genotypes at stages of plant growth. J Agron Crop Sci 190: 6–12.
Diepenbrock W (2003). Yield analysis of winter oilseed rape (Brassica napus L.): a review. Field Crop Res 67: 35–49.
Duan W, Yu Z, Zhang Y, Wang D, Shi Y, Xu Z (2014). Effects of nitrogen application on biomass accumulation, remobilization and soil water contents in rainfed wheat field. Turk J Field Crops 19: 25–34.
Filippou P, Antoniou C, Fotopoulos V (2011). Effect of drought and rewatering on the cellular status and antioxidant response of Medicago truncatula plants. Plant Signal. Behav 6: 270–277.
Hanson A, Nelson CE, Everson EH (1977). Evaluation of free proline accumulation as an index of drought resistance using two contrasting barley cultivars. Crop Sci 17: 720–726.
Hasegawa PM, Bressan RA, Nelson DE, Samaras Y, Rhodes D (1994). Tissue culture in the improvement of salt tolerance in plants. In: Soil Mineral Stresses. Berlin, Heidelberg: Springer, pp. 83–125.
Iannucci A, Rascio A, Russo M, Di Fonzo N, Martiniello P (2000). Physiological responses to water stress following a conditioning period in berseem clover. Plant and Soil 223: 219–229.
Ibrahim MH, Jafar HZE, Rahmat A, Abdul Rahman Z (2011). Involvement of nitrogen on flavonoids, glutathione, anthocyanin, ascorbic acid and antioxidant activities of Malaysian medicinal plant Labisia pumila Blume (Kacip Fatimah). Int J Mol Sci 13: 393–408.
Jiang ZW, Lin WX, Li YZ, Zhuo CY, Yang HJ, Xie HA (2005). Effects of nitrogen fertilizer rates on some physiological characteristics of ratoon rice. Fujian J Agric Sci 3: 168–171.
Kamkar B, Daneshmand AR, Ghooshchi F, Shiranirad AH, Safahani Langeroudi AR (2011). The effects of irrigation regimes and nitrogen rates on some agronomic traits of canola under a semiarid environment. Agr Water Manage 98: 1005–1012.
Kappen L, Hammler A, Schultz G (1998). Seasonal changes in the photosynthetic capacity of winter rape plants under different nitrogen regimes measured in the field. J Agron Crop Sci 181: 179–187.
Kar M, Mishra D (1976). Catalase, peroxidase and polyphenol oxidase activity during rice leaf senescence. Plant Physiol 57: 315–319.
Kishor PBK, Hong Z, Miao GH, Hu CAA, Verma DPS (1995). Overexpression of [delta]-1-pyrroline-5-carboxylate synthetase increases proline production and confers osmotolerance in transgenic plants. Plant Physiol 108: 1387–1394.
Li SX (2007). Dryland Agriculture in China. Beijing, China: Science Press, pp. 42–444.
Liu J, Zhu JK (1997). Proline accumulation and salt-stress-induced gene expression in a salt-hypersensitive mutant of Arabidopsis. Plant Physiol 114: 591–596.
Majid A, Simpson GM (1997). Growth relationships of mustard under irrigated conditions. Pakistan J Bot 29: 125–134.
Mencon IM, Sgherric LM, Pinzino C (1995). Activated oxygen production and detoxification in wheat plants subjected to a water deficit programme. J Exp Bot 46: 1123–1130.
Mohammadian R, Moghaddam M, Rahimian H, Sadeghian SY (2005). Effect of early season drought stress on growth characteristics of sugar beet genotypes. Turk J Agric For 29: 357–368.
Pidgeon JD, Werker AR, Jaggard KW, Richter GM, Lister DH, Jones PD (2001). Climatic impact on the productivity of sugar beet in Europe. Agr Forest Meteorol 109: 27–37.
Ramachandra RA, Chaitanya KV, Jutur PP, Sumithra K (2004). Differential anti-oxidative responses to water stress among five mulberry (Morusalba L.) cultivars. Environ Exp Bot 52: 33–42.
Rensburg LV, Krüger GHJ (1994). Applicability of abscisic acid and (or) proline accumulation as selection criteria for drought tolerance in Nicotiana tabacum. Can J Bot 72: 1535–1540.
Sairam RK, Srivastava GC (2001). Water stress tolerance of wheat (Triticum aestivum L.): variations in hydrogen peroxide accumulation and antioxidant activity in tolerant and susceptible genotypes. J Agron Crop Sci 186: 63–70.
Saneoka H, Moghaieb REA, Premachangra GS, Fujita K (2004). Nitrogen nutrition and water stress effects on cell membrane stability and leaf water relations in Agrostispolus trishuds. Environ Exp Bot 52: 131–138.
Shin R, Berg RH, Schachtman DP (2005). Reactive oxygen species and root hairs in Arabidopsis root response to nitrogen, phosphorus and potassium deficiency. Plant Cell Physiol 46: 1350–1357.
Sinclair TR, Vadez V (2002). Physiological traits for crop yield improvement in low N and P environments. Plant and Soil 245: 1–15.
Smirnoff N (1993). The role of active oxygen in the response of plants to water deficit and desiccation. New Phytologist 52: 27–58.
Sudhakar C, Lakshmi A, Giridarakumar S (2001). Changes in the antioxidant enzyme efficacy in two high yielding genotypes of mulberry under NaCl salinity. Plant Sci 161: 613–619.
Sun Q, Liang ZS, Wang WL, Li XJ, Zhang FS (2001). Effects of mechanism lipid peroxidation and photosynthesis rate on water stress under different nitrogen level in maize. Acta Agric. Boreal-Occident Sin. 1: 7–10.
Tanou G, Molassiotis A, Diamantidis G (2009). Induction of reactive oxygen species and necrotic death-like destruction in strawberry leaves by salinity. Environ Exp Bot 65: 270–281.
Tognetti R, Palladino M, Minnocci A, Delfine S, Alvino A (2003). The response of sugar beet to drip and low-pressure sprinkler irrigation in southern Italy. Agr Water Manage 60: 135–155.
Toker C, Gorham J, Cağırgan MI (2009). Certain ion accumulations in barley mutants exposed to drought and salinity. Turk J Field Crops 14: 162–169.
Xu ZZ, Yu ZW, Wang D, Zhang YL (2005). Nitrogen accumulation and translocation for winter wheat under different irrigation regimes. J Agron Crop Sci 191: 439–449.
Yeo A (1998). Molecular biology of salt tolerance in the context of whole-plant physiology. J Exp Bot 49: 915–929.
Zaman A, Das PK (1991). Effect of irrigation and nitrogen on yield and quality of safflower. Indian J Agron 36: 177–179.
Zhang LX, Li SX, Zhang H, Liang ZS (2007). Nitrogen rates and water stress effects on production, lipid peroxidation and antioxidative enzyme activities in two maize (Zea mays L.) genotypes. J Agron Crop Sci 193: 387–397.
Zhang SG, Liu GL (2001). Plant nutrition and drought resistance of crops. Chinese Bulletin of Botany 18: 64–69.
Zhao LY, Dong XP, Shan L (2005). The response mechanism of active oxygen species removing system to drought stress. Acta Botanica Boreali-Occidentalia Sinica 25: 413–418.
Zhou J H, Wang YT, Yu JB, Cheng D (2001). Effect of nitrogen form on the activities of protective enzyme in tobacco leaves under drought condition. J Chin Tobacco Sci 2: 5–8.