Anther Culture Potential of Linseed ( Linum usitatissimumL.): Effects of Genotypes and Pretreatment on Callus Formation and Differentiation

This study is concerned with the production of haploids of linseed. Anthers of eight genotypes were cultured both on solid and in liquid medium. It was found that callus induction rate was low in both media and also the cultivars significantly affected callus induction rate both on solid and in liquid medium. The cultivar Blue-Chip gave, on average, the maximum (3.67%) callus induction rate on solid medium and the cultivar Antares gave, on average, the maximum (3.35%) callus induction rate in liquid medium. The cultivar Norman produced callus on solid medium but did not produce any callus in liquid medium. On the other hand, the cultivar McGregor did not produce any callus in either solid or liquid medium. Pre-treatment of flower buds with cold significantly reduced callus induction rate by about four fold compared with the no pre-treatment on solid medium but increased (not statistically significant) callus induction rate by 25.8% in liquid medium. Although some callus induction was achieved in both on solid and in liquid media, no differentiation was observed when calli were transferred to regeneration medium.

Anther Culture Potential of Linseed ( Linum usitatissimumL.): Effects of Genotypes and Pretreatment on Callus Formation and Differentiation

This study is concerned with the production of haploids of linseed. Anthers of eight genotypes were cultured both on solid and in liquid medium. It was found that callus induction rate was low in both media and also the cultivars significantly affected callus induction rate both on solid and in liquid medium. The cultivar Blue-Chip gave, on average, the maximum (3.67%) callus induction rate on solid medium and the cultivar Antares gave, on average, the maximum (3.35%) callus induction rate in liquid medium. The cultivar Norman produced callus on solid medium but did not produce any callus in liquid medium. On the other hand, the cultivar McGregor did not produce any callus in either solid or liquid medium. Pre-treatment of flower buds with cold significantly reduced callus induction rate by about four fold compared with the no pre-treatment on solid medium but increased (not statistically significant) callus induction rate by 25.8% in liquid medium. Although some callus induction was achieved in both on solid and in liquid media, no differentiation was observed when calli were transferred to regeneration medium.
Turkish Journal of Agriculture and Forestry-Cover
  • ISSN: 1300-011X
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler

Investigations on Repellent and Insecticidal Effects of Xanthium strumarium L. on Colorado Potato Beetle Leptinotrasa decemlineata Say (Col: Chrysomelidae)

Suzan ÇETİNSOY, Ali TAMER

The Opportunities of Using Mushroom Compost Waste in Strawberry Growing

Ahsen İşık ÖZGÜVEN

Effect of Nitrogen and Phosphorus Fertilization on the Yield and Nutrient Status of Rice Crop Grown on Artificial Siltation Soil From the Kelkit River

A. Reşit BROHİ, M. Rüştü KARAMAN, Arif AKTAS, Erdinç SAVAŞLI

Determination of the Utilization of Nitrogen From Tobacco Waste By Wheat Crop With 15 N Tracer Technique

A. Reşit BROHİ, M. Rüştü KARAMAN

Cultivar, Weed and Row Spacing Effects on Some Agronomic Characters of Safflower ( Carthamus tinctorius L.) in Spring Planting

Nevzat USLU, Ali AKIN, M. Basri HALİTLİGİL

Effect of nitrogen and phosphorus fertilization on the yield and nutrient status of rice crop grown on artificial siltation soil from the Kelkit River*

A. Reşit BROHİ, Erdinç SAVAŞLI, M. Rüştü KARAMAN, Arif AKTAŞ

A Study on the Lipase-Catalayzed Esterification in Organic Solvent

Mustafa Ümit ÜNAL

Response to Inoculation and Sowing Date of Soybean Under Bafra Plain Conditions in the Northern Region of Turkey

Nevzat USLU

Determination of the utilization of nitrogen from tobacco waste by wheat crop with $^{15}N$ tracer technique*

A. Reşit BROHİ, Akgün AYDENİZ, M. Rüştü karaman

Influence of Natural Zeolite on Performance of Laying Hens and Egg Quality

Ergin ÖZTÜRK, Güray ERENER, Musa SARICA