Fariba MEHRKHOU, Nurper GUZ, Ertan Mahir KORKMAZ, Naciye Sena CAGATAY

Analysis of genetic variation in an important pest, Tuta absoluta, and its microbiota with a new bacterial endosymbiont

Tomato leaf miner is an aggressive and invasive pest that causes serious damage in tomato production, resulting in up to 100% yield losses during serious outbreaks. The management of tomato leaf miner with synthetic pesticides is difficult due to its leaf mining habit, short life span, high reproductive rates, and insecticide resistance problem. In this study, sequences of the mitochondrial cytochrome oxidase I (mtCOI) gene of Tuta absoluta specimens were analyzed and compared with the available COI sequence data members from GenBank. All the specimens of tomato leaf miner were clustered together in a single group with a high support value, suggesting the presence of a homogeneity in the T. absoluta specimens. Furthermore, 164 samples were analyzed in order to detect the prevalence of Arsenophonus, Cardinium, Hamiltonella, Pantoea, Spiroplasma,and Wolbachia infection in T. absoluta populations. PCR analysis revealed 100% prevalence of Wolbachia and Pantoea; however, no other symbiotic bacterial infections were observed in T. absoluta populations. Finally, Wolbachia wsp, Pantoea 16S ribosomal RNA genes, and mtDNA COI haplotypes were analyzed in order to estimate the mitochondrial effects of these endosymbionts on the population structure of tomato leaf miner. The findings of high genetic homogeneity among T. absoluta populations with high prevalence of Wolbachia and Pantoea infections seem to imply that both bacterial infections have less impact on the mtDNA variations.

Keywords:

Endosymbionts, genetic variation, mitochondrial DNA, tomato leaf miner,

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Ahmed MZ, 2016, BMC EVOL BIOL, V16, DOI 10.1186/s12862-016-0660-x
Baldo L, 2006, APPL ENVIRON MICROB, V72, P7098, DOI 10.1128/AEM.00731-06
Ballard JWO, 1996, GENETICS, V144, P1519
Barati R, 2018, J ECON ENTOMOL, V111, P1551, DOI 10.1093/jee/toy134
Bennett GM, 2015, P NATL ACAD SCI USA, V112, P10169, DOI 10.1073/pnas.1421388112
Bettaibi A, 2016, J GENET, V93, pE110, DOI 10.1007/s12041-013-0310-7
Bozorov TA, 2019, SCI REP-UK, V9, DOI 10.1038/s41598-019-41368-x
Braig HR, 1998, J BACTERIOL, V180, P2373, DOI 10.1128/JB.180.9.2373-2378.1998
Broderick NA, 2004, APPL ENVIRON MICROB, V70, P293, DOI 10.1128/AEM.70.1.293-300.2004
Carvalho GA, 2018, J APPL ENTOMOL, V142, P905, DOI 10.1111/jen.12531
Charlat S, 2009, BMC EVOL BIOL, V9, DOI 10.1186/1471-2148-9-64
Chen J, 2016, SCI REP-UK, V6, DOI 10.1038/srep28484
Chevasco V, 2012, CONSERV GENET RESOUR, V4, P159, DOI 10.1007/s12686-011-9498-0
Chiel E, 2007, B ENTOMOL RES, V97, P407, DOI 10.1017/S0007485307005159
Choi JY, 2015, GENOME BIOL EVOL, V7, P2362, DOI 10.1093/gbe/evv158
Cifuentes D, 2011, PEST MANAG SCI, V67, P1155, DOI 10.1002/ps.2166
Doyle J.J., 1987, PHYTOCHEM B BOT SOC, V19, P11
Duman M, 2015, BIOCHEM SYST ECOL, V59, P70, DOI 10.1016/j.bse.2015.01.003
Duplouy A, 2018, PEERJ, V6, DOI 10.7717/peerj.4629
Duric Zorica, 2014, Polish Journal of Entomology, V83, P121, DOI 10.2478/pjen-2014-0009
EPPO, 2005, EPPO B .
Feldhaar H, 2011, ECOL ENTOMOL, V36, P533, DOI 10.1111/j.1365-2311.2011.01318.x
Flores LV, 2003, BAG-J BASIC APPL GEN, V15, P47
Fujii Y, 2001, P ROY SOC B-BIOL SCI, V268, P855, DOI 10.1098/rspb.2001.1593
Glowska E, 2015, INFECT GENET EVOL, V30, P140, DOI 10.1016/j.meegid.2014.12.019
Graham RI, 2012, BMC EVOL BIOL, V12, DOI 10.1186/1471-2148-12-204
Guedes RNC, 2019, J PEST SCI, V92, P1329, DOI 10.1007/s10340-019-01086-9
Guillemaud T, 2015, SCI REP-UK, V5, DOI 10.1038/srep08371
Guindon S, 2010, SYST BIOL, V59, P307, DOI 10.1093/sysbio/syq010
Gurung K, 2019, ENTOMOL EXP APPL, V167, P156, DOI 10.1111/eea.12768
Guz N, 2020, CURR MICROBIOL, V77, P1525, DOI 10.1007/s00284-020-01969-6
Guz N, 2013, BIOCHEM SYST ECOL, V48, P85, DOI 10.1016/j.bse.2012.12.010
Hagenblad J, 2015, BMC GENET, V16, DOI 10.1186/s12863-015-0242-8
Hammer TJ, 2014, PLOS ONE, V9, DOI 10.1371/journal.pone.0086995
Hassan M.N., 2009, INT PEST CONTROL, V51, P85
Hawley DM, 2006, MOL ECOL, V15, P263, DOI 10.1111/j.1365-294X.2005.02767.x
He C, 2013, J INSECT SCI, V13, DOI 10.1673/031.013.16501
Hebert Paul D. N., 2003, Proceedings of the Royal Society Biological Sciences Series B, V270, pS96
Hilgenboecker K, 2008, FEMS MICROBIOL LETT, V281, P215, DOI 10.1111/j.1574-6968.2008.01110.x
Hiroki M, 2002, NATURWISSENSCHAFTEN, V89, P167, DOI 10.1007/s00114-002-0303-5
Hornett EA, 2008, EVOLUTION, V62, P1258, DOI 10.1111/j.1558-5646.2008.00353.x
Hurst GDD, 2005, P ROY SOC B-BIOL SCI, V272, P1525, DOI 10.1098/rspb.2005.3056
Ilinsky Y, 2017, MOL PHYLOGENET EVOL, V109, P164, DOI 10.1016/j.ympev.2016.12.034
Jiggins FM, 2001, HEREDITY, V86, P161, DOI 10.1046/j.1365-2540.2001.00804.x
Kageyama D, 2004, P ROY SOC B-BIOL SCI, V271, P251, DOI 10.1098/rspb.2003.2604
Kageyama D, 2003, GENOME, V46, P983, DOI [10.1139/g03-082, 10.1139/G03-082]
Karaagac SU, 2015, PHYTOPARASITICA, V43, P693, DOI 10.1007/s12600-015-0476-z
Kearse M, 2012, BIOINFORMATICS, V28, P1647, DOI 10.1093/bioinformatics/bts199
Keeling MJ, 2003, HEREDITY, V91, P382, DOI 10.1038/sj.hdy.6800343
Kenyon LJ, 2015, GENOME BIOL EVOL, V7, P620, DOI 10.1093/gbe/evv006
Kinyanjui G, 2019, J ECON ENTOMOL, V112, P872, DOI 10.1093/jee/toy357
Kontsedalov S, 2008, PEST MANAG SCI, V64, P789, DOI 10.1002/ps.1595
Korkmaz EM, 2011, TURK J BIOL, V35, P713, DOI 10.3906/biy-1003-65
Krenn HW, 2010, ANNU REV ENTOMOL, V55, P307, DOI 10.1146/annurev-ento-112408-085338
Krishnamurthy PK, 2012, BIODIVERS CONSERV, V21, P1901, DOI 10.1007/s10531-012-0306-2
Kumar S, 2018, MOL BIOL EVOL, V35, P1547, DOI 10.1093/molbev/msy096
Lauzon CR, 2009, ANN ENTOMOL SOC AM, V102, P85, DOI 10.1603/008.102.0109
Librado P, 2009, BIOINFORMATICS, V25, P1451, DOI 10.1093/bioinformatics/btp187
Lietti Marcela M.M., 2005, Neotrop. entomol., V34, P113, DOI 10.1590/S1519-566X2005000100016
Lindholm AK, 2005, MOL ECOL, V14, P3671, DOI 10.1111/j.1365-294X.2005.02697.x
Maccollom GB, 2009, ENVIRON ENTOMOL, V38, P116, DOI 10.1603/022.038.0114
Mansour R, 2018, ENTOMOL GEN, V38, P83, DOI 10.1127/entomologia/2018/0749
Megido RC, 2012, J PEST SCI, V85, P409, DOI 10.1007/s10340-012-0458-6
Meyer CP, 2003, BIOL J LINN SOC, V79, P401, DOI 10.1046/j.1095-8312.2003.00197.x
Moran NA, 2008, ANNU REV GENET, V42, P165, DOI 10.1146/annurev.genet.41.110306.130119
Peakall R, 2006, MOL ECOL NOTES, V6, P288, DOI 10.1111/j.1471-8286.2005.01155.x
Pereyra PC, 2006, NEOTROP ENTOMOL, V35, P671, DOI 10.1590/S1519-566X2006000500016
Pinto-Tomas AA, 2011, ENVIRON ENTOMOL, V40, P1111, DOI 10.1603/EN11083
Richardson MF, 2012, PLOS GENET, V8, DOI 10.1371/journal.pgen.1003129
Robinson CJ, 2010, MICROB ECOL, V59, P199, DOI 10.1007/s00248-009-9595-8
Russell B, 2012, ELECTRON J HEALTH IN, V7
Saccone C, 2000, GENE, V261, P153, DOI 10.1016/S0378-1119(00)00484-4
Saidov N, 2018, FLA ENTOMOL, V101, P147, DOI 10.1653/024.101.0129
Salunke BK, 2012, APPL ENVIRON MICROB, V78, P4458, DOI 10.1128/AEM.07298-11
Sasaki T, 2002, GENETICS, V162, P1313 .
Satishchandra NK, 2019, J APPL ENTOMOL, V143, P518, DOI 10.1111/jen.12622
Schuler H, 2018, BMC EVOL BIOL, V18, DOI 10.1186/s12862-018-1151-z
Schuler H, 2016, MOL ECOL, V25, P1595, DOI 10.1111/mec.13571
Sevim A, 2016, ANN MICROBIOL, V66, P749, DOI 10.1007/s13213-015-1155-5
Shashank PR, 2018, 3 BIOTECH, V8, DOI 10.1007/s13205-018-1374-0
Siqueira H. A. A., 2000, Agricultural and Forest Entomology, V2, P147, DOI 10.1046/j.1461-9563.2000.00062.x
Skaljac M, 2012, AFR ENTOMOL, V20, P191, DOI 10.4001/003.020.0125
SOLIGNAC M, 1994, CR ACAD SCI III-VIE, V317, P461
Staudacher H, 2016, PLOS ONE, V11, DOI 10.1371/journal.pone.0154514
Stouthamer R, 1999, ANNU REV MICROBIOL, V53, P71, DOI 10.1146/annurev.micro.53.1.71
Tabuloc CA, 2019, J PEST SCI, V92, P1397, DOI 10.1007/s10340-019-01116-6
Tagami Y, 2004, INSECT MOL BIOL, V13, P359, DOI 10.1111/j.0962-1075.2004.00492.x
Tang X., 2012, PLOS ONE, V7, P7 .
Telschow A, 2019, FRONT GENET, V10, DOI 10.3389/fgene.2019.00062
Thao ML, 2004, APPL ENVIRON MICROB, V70, P3401, DOI 10.1128/AEM.70.6.3401-3406.2004
Tsuchida T, 2002, MOL ECOL, V11, P2123, DOI 10.1046/j.1365-294X.2002.01606.x
Tsutsui ND, 2000, P NATL ACAD SCI USA, V97, P5948, DOI 10.1073/pnas.100110397
TURELLI M, 1992, GENETICS, V132, P713 .
van Meer MMM, 1999, INSECT MOL BIOL, V8, P399, DOI 10.1046/j.1365-2583.1999.83129.x
Voirol LRP, 2018, FRONT MICROBIOL, V9, DOI 10.3389/fmicb.2018.00556
Walterson AM, 2015, FEMS MICROBIOL REV, V39, P968, DOI 10.1093/femsre/fuv027
Weeks AR, 2003, P ROY SOC B-BIOL SCI, V270, P1857, DOI 10.1098/rspb.2003.2425
Weinert LA, 2015, P ROY SOC B-BIOL SCI, V282, DOI 10.1098/rspb.2015.0249
Werren JH, 1997, ANNU REV ENTOMOL, V42, P587, DOI 10.1146/annurev.ento.42.1.587
Werren JH, 1997, INFLUENTIAL PASSENGERS, P1
Werren JH, 1995, P ROYAL SOC B, V261 .
Xia XF, 2017, FRONT MICROBIOL, V8, DOI 10.3389/fmicb.2017.00663
Yalcin M, 2015, TURK ENTOMOL DERG-TU, V39, P137
Yatkin Gokhan, 2018, Yuzuncu Yil Universitesi Tarim Bilimleri Dergisi, V28, P126
Yukselbaba Utku, 2016, Akdeniz Universitesi Ziraat Fakultesi Dergisi, V29, P5
Yun JH, 2014, APPL ENVIRON MICROB, V80, P5254, DOI 10.1128/AEM.01226-14
Zibaee Idin, 2016, Arthropods, V5, P77 .