Esra UÇAR SÖZMEN, Yaşar ÖZYİĞİT, Begüm TÜTÜNCÜ, Kenan TURGUT

Agronomic and chemical performance of selected Origanum dubium Boiss. clones for industrial use

Origanum dubium Boiss. is an economically important wild oregano species that is intensely collected from the natural floraof Antalya, Turkey. This herb is used mainly to produce an essential oil due to its high yield and high carvacrol content. The aim of thisstudy was to improve the biomass yield and essential oil and carvacrol content of O. dubium for industrial use. In preliminary studies,a basic population was established using seeds collected from wild plants and then trials of A and B clones were run. Dry biomass yieldper A clone plant and essential oil content varied at 5.05–40.86 g and 5%–14%, respectively. Carvacrol was the main constituent in allsamples and varied from 72.26% to 88.21%. Dry biomass yield per B clone plant was 21.64–95.52 g, dry leaf yield per plant was 14.80–48.77 g, essential oil content was 6%–9%, and carvacrol rate was 80.99%–88.46%. After evaluating the B clones, 10 clones were selectedand they were planted in a plot with a randomized block design and three replications in Antalya in 2014. In this trial, clone 7 producedthe highest biomass yields per plot (2874.33 g fresh and 1185.00 g dry) and the highest fresh (2379.67 g) and dry (795.33 g) leaf yieldsper plot. Essential oil content varied from 7.97% to 11.16%. Clone 137 had the best essential oil content (11.16%), followed by clone 7(10.76%) and clone 119 (10.59%). Clone 7 appeared to be superior to the others in terms of agronomic features, followed by clone 119.Essential oil and carvacrol rates were 10.76% and 85.02% in clone 7 and 10.59% and 91.04% in clone 119, respectively. Consequently,clones 7 and 119 appeared to be superior to the others.

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Ahmad A, Khan A, Akhtar F, Yousuf S, Xess I, Khan LA, Manzoor N (2011). Fungicidal activity of thymol and carvacrol by disrupting ergosterol biosynthesis and membrane integrity against Candida. Eur J Clin Microbiol Infect Dis 30: 41-50.
Arnold N, Bellomaria B, Valentini G, Arnold HJ (1993). Comparative study of the essential oils from three species of Origanum growing wild in the eastern Mediterranean region. J Essent Oil Res 5: 71-77.
Baser KHC, Kirimer N, Tumen G (1993). Composition of the essential oil of Origanum majorana L. from Turkey. J Essent Oil Res 5: 577-579.
Ceylan A, Bayram E, Sahbaz N, Otan H, Karaman S (2003). Yield performance and essential oil composition of individual plants and improved clones of Origanum onites L. grown in the Aegean region of Turkey, Isr J Plant Sci 51: 285-290.
Dambolena JS, Zygadlo JA, Rubinstein H (2011). Antifumonisin activity of natural phenolic compounds: a structure-propertyactivity relationship study. Int J Food Microbiol 145: 140-146.
Figuérédo G, Cabassu P, Chalchat JC(2006). Studies of Mediterranean oregano populations. VII: Chemical composition of essential oils of carvacrol-rich oregano of various origins. J Essent Oil Res 18: 244-249.
Franz C, Novak J (1996) Breeding of Origanum Species, Proceedings of the IPGRI International Workshop on Oregano, 8–12 May 1996, CIHEAM, Valenzano, Bari, Italy. Rome, Italy: IPGRI, pp. 49-56.
Karioti A, Vrahimi-Hadjilouca T, Droushiotis D, Rancic A, Hadjipavlou-Litina, DS, Skaltsa H (2006). Analysis of the essential oil of Origanum dubium growing wild in Cyprus. Investigation of its antioxidant capacity and antimicrobial activity. Planta Med 72: 1330-1334.
Koparal AT, Zeytinoglu M (2003). Effects of carvacrol on a human non-small cell lung cancer (NSCLC) cell line, A549. Cytotechnology 43: 149-154.
Lukas B, Samuel R, Novak J (2010). Oregano or marjoram? The enzyme γ-terpinene synthase affects chemotype formation in the genus Origanum. Isr J Plant Sci 58: 211-220.
Lukas B, Samuel R, Mader E, Baser KHC, Duman H, Novak J (2013). Complex evolutionary relationships in Origanum section Majorana (Lamiaceae). Bot J Linn Soc 171: 667-686.
Mezzoug N, Elhadri A, Dallouh A, Amkiss S, Skali NS, Abrini J, Zhiri A, Baudoux D, Diallo B, El Jaziri M (2007). Investigation of the mutagenic and antimutagenic effects of Origanum compactum essential oil and some of its constituents. Mutat Res 629: 100- 110.
Nostro A, Papalia T (2012). Antimicrobial activity of carvacrol: current progress and future prospectives. Recent Pat Antiinfect Drug Discov 7: 28-35.
Sarer E, Scheffer JJC, Svedsen AB (1982). Monoterpenes in the essential oil of Origanum majorana. Planta Med 46: 236-23.
Skoula M, Harborne JB (2002). The taxonomy and chemistry of Origanum. In: Kintzios SE, editor. Oregano: the genera Origanum and Lippia. London, UK: Taylor and Francis, pp. 67-108.
Tang X, Chen S, Wang L (2011). Purification and identification of carvacrol from the root of Stellera chamaejasme and research on its insecticidal activity. Nat Prod Res 25: 320-325.
Turgut K, Ozyigit Y, Ucar E, Tutuncu B (2013). Breeding of wild marjoram (Origanum majorana L.) for essential oil production. 61st International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research, 1–5 September 2013, Munster, Planta Medica 79: 1275.
Vera RR, Chane-Ming J (1999). Chemical composition of the essential oil of marjoram (Origanum majorana L.) from Reunion Island. Food Chem 66: 143-145.