Reham TAHTAMOUNI, Rida SHIBLI, Ayed AL-ABDALLAT, Tamara AL-QUDAH

91100

Analysis of growth, oil yield, and carvacrol in Thymbra spicata L. after slow-growth conservation

Growth, oil yield, and carvacrol content in Thymbra spicata L. Var. Spicata microshoots were analyzed after 12 weeks of storage in MS media supplemented with elevated levels (0.0, 0.1, 0.2, 0.3, and 0.4 M) of three osmotic agents (sucrose, sorbitol, and mannitol) or ABA (0.0, 3.8, 7.6, and 11.4 μM). The obtained results revealed that using 0.2 M sucrose in the storage media yielded the best results in terms of growth reduction during conservation as well as maintenance of microshoot recovery at full capacity (100%) after conservation. Meanwhile, 0.1 M sorbitol or mannitol in addition to 3.8 μM ABA were able to reduce microshoot growth during conservation, but this was at the expense of microshoot recovery after subculture to normal tissue culture conditions. The results also showed that total yield of volatile oils extracted from the dried microshoots prestored in 0.2 M sucrose produced 6.0% oil yield, which was very close to the quantity obtained in the wild mother plant and much greater than the oil yields extracted from the microshoots prestored in the other conservation treatments. Similarly, the maximum carvacrol level (9.7 × 10-4 M) was obtained in microshoots prestored in 0.2 M, sucrose which was even higher than the carvacrol amount extracted from the wild mother plant itself.
Keywords:

Carvacrol conservation, in vitro, oil yield, osmotic agents,

PDF
Turkish Journal of Agriculture and Forestry-Cover
  • ISSN: 1300-011X
  • Yayın Aralığı: 6
  • Yayıncı: TÜBİTAK
Arşiv
Sayıdaki Diğer Makaleler

The effect of fire on the vegetation and soil properties of ungrazed shortgrass steppe rangeland of the Eastern Anatolia region of Turkey

Şule ERKOVAN, Serdar BİLEN, M. Kerim GÜLLAP, H. İbrahim ERKOVAN, Ali KOÇ

Characterization of some bioactive compounds and physicochemical properties of grape varieties grown in Turkey: thermal degradation kinetics of anthocyanin

Osman SAĞDIÇ, Salih KARASU, Mehmet BAŞLAR, Mahmut KILIÇLI, Safa KARAMAN, Ahmet Abdullah US, Hasan YAMAN

Determination of fatty acids and volatile compounds in fruits of rosehip (Rosa L.) species by HS-SPME/GC-MS and Im-SPME/GC-MS techniques

Mozhgan ZARIFIKHOSROSHAHI, Nesibe Ebru KAFKAS, Zehra Tuğba MURATHAN

Yusuf KURT, John FRAMPTON, Fikret ISIK, Chal LANDGREN, Gary CHASTAGNER

Analysis of dendrometric diversity among natural populations of cork oak (Quercus suber L.) from Morocco

Amal LAAKILI, Bouchra BELKADI, Fatima GABOUN, Chaimaa YATRIB, Mohamed MAKHLOUFI, Salwa ANTRY EL, Leila MEDRAOUI, Ahmed LAAMARTI, Abdelkarim FILALI-MALTOUF

Halina BUCZKOWSKA, Zenia MICHALOJC, Renata NURZYNSKA-WIERDAK

Virulence and horizontal transmission of Beauveria pseudobassiana S.A. Rehner & Humber in Ips sexdentatus and Ips typographus (Coleoptera: Curculionidae)

SEDA KOCAÇEVİK, ALİ SEVİM, MAHMUT EROĞLU, ZİHNİ DEMİRBAĞ, İSMAİL DEMİR

Improving nitrogen-use and radiation-use efficiencies of C4 summer cereals by split nitrogen applications under an irrigated arid environment

Hülya DOĞAN, Naeem SARWAR, Muhammad ZIA-UL-HAQ, Ahmad Naeem SHAHZAD, Khadim HUSSAIN, Umair FAROOQ, Shahzad Usman KHAN, Atique-ur REHMAN, Muhammad Tauseef SULTAN, Abdul WAHEED, Muhammad Azam KHAN, Shabbir HUSSAIN, Shakeel AHMAD, Hakoomat ALI

Impact of long term soil management practices on the fertility and weed flora of an almond orchard

Giovanna CUCCI, Giovanni LACOLLA, Carmine CRECCHIO, Silvia PASCAZIO, Donato GIORGIO DE

Variation in needle and cone characteristics and seed germination ability of Abies bornmuelleriana and Abies equi-trojani populations from Turkey

John FRAMPTON, Fikret ISIK, Chal LANDGREN, Gary CHASTAGNER, Yusuf KURT