Investigations were carried out with five commercial oriental aromatic tobacco varieties of the type Prilep: P – 23, PV 156/1, NS – 72, P – 66 – 9/7 and P – 79 – 94, to study green mass yield per stalk. The trial was set up in the Experimental field of Tobacco Institute – Prilep in 2010, 2011 and 2012, in randomized block design with four replications. Traditional agricultural practices were applied for realization of the experiment. The aim of investigations was through biometric analysis of the above quantitative trait to evaluate the variability of the commercial varieties, and thus give an assessment of their stability and guidance for their further expansion and maintenance. No significant differences were observed between the three years of investigation, which is an indication that green mass yield per stalk is highly heritable traits and varietal characteristic. Statistical parameters of variability are low, which is an indication of stable and homozygous genotypes, adapted to agro–ecological conditions of the region. Results on the standard deviation and variability coefficient were lower in 2012, because the seed sawn in this crop was obtained from one stalk for each variant isolated in 2010 and again from one stalk isolated in 2011. Varieties PV 156/1 and NS – 72 have approximately the same average value for the trait, but the statistical indicators of the variability is lower in PV 156/1. From the dates it is concluded that the investigated varieties are stable and the most stable is P – 66 – 9/7, which is also with the highest yield. The findings of this paper imply a basis for further research in different locations and different application of statistical models for assessing interactions GxL (genotype x location) and GxY (genotype x year), which would have completed the image about stability of commercial varieties in Republic of Macedonia