Işıl SARAÇ SİVRİKAYA, Bekir TOSUN, Ersin KARAKAYA

Origanum onites L. ve Rosmarinus officinalis L. Uçucu Yağlarının Kimyasal İçeriklerinin ve Fusarium solani’ ye Karşı Antifungal Aktivitelerinin Belirlenmesi

Bu çalışma, kekik (Origanum onites L.) ve biberiye (Rosmarinus officinalis L.) bitkilerinden elde edilen uçucu yağların Fusarium solani‘ye karşı antifungal aktivitesini tespit etmek amacıyla laboratuvar ortamında yürütülmüştür. O. onites ve R. officinalis türlerinin herba kısımlarından uçucu yağlar elde edilerek, yağların bileşenleri tanımlanmıştır. Elde edilen uçucu yağlar 1, 2 ve 4 μl ml-1 dozunda otoklav edilen Patates Dekstroz Agar (PDA) besi yerine ilave edilmiştir. Patojenlere ait 8 mm çapında miselyum diskleri PDA besi yerlerine ekilmiştir. Kontrol grubu olarak uçucu yağlardan ari PDA besi yeri kullanılmıştır. PDA’lı petriler 24±1oC’da 7 gün inkübasyona bırakılmıştır. İnkübasyon sonrasında fungusların koloni çapları ölçülerek, kontrol grubuna göre bitki uçucu yağlarının % engelleme oranları hesaplanmıştır. Çalışmadan elde edilen verilere göre, Rosmarinus officinalis L. uçucu yağının yüksek dozu (4 μl ml-1 ) test edilen Fusarium solani’ye karşı %71,72 etki göstermiş ve doz seviyesi arttıkça antifungal etkinin de arttığı belirlenmiştir. Origanum onites L. uçucu yağı, uygulanan üç dozda Fusarium solani’nin misel gelişiminde %78,91-%82,70 oranları arasında engelleme göstermiştir. Bu sonuçlar bitkilerden elde edilen uçucu yağların sentetik fungisitlere alternatif olabileceğini göstermiştir.

Determination of Chemical Contents of Origanum onites L. and Rosmarinus officinalis L. Essential Oils and Determination of Antifungal Activities Against Fusarium solani

This study was conducted in vitro to determine the antifungal effect of essential oils obtained from Origanum onites L. and Rosmarinus officinalis L. plant species on Fusarium solani. Essential oils were obtained from the herba parts of O. onites and R. officinalis species and the components of the oils were identified. The essential oils were added to Potato Dextrose Agar (PDA) at a dose of 1, 2 and 4 μl ml-1 . 8 mm diameter mycelium discs belonging to pathogens were planted in PDA media. PDA medium free from essential oils was used as the control group. PDA petri dishes were incubated at 24 ± 1 o C. In the antifungal study, mycelium diameters in the petri dishes were measured by automatic caliper at the end of the 7th day of incubation period. As a result, essential oil of Rosmarinus officinalis L. was inhibited mycelial growth of Fusarium solani at a dose of 4 μl ml-1 by 71,72%. The study indicated that Origanum onites L. inhibited the mycelium development of Fusarium solani between 78.91% and 82.70%. These results showed that essential oils obtained from these plants can be an alternative control method against pathogens.

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