Vladimir KRASTEV, Gancho PASSEV, Dimitrina KOSTOVAPROTOCHRISTOVA, - -

Identification of I-gene for BCMV Resistance with Scar Marker SW13 in Phaseolus vulgaris L. Generations, Derived from the Cross (BAT 477 X Dobroudjanski Ran)

Anahtar Kelimeler:

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Identification of I-gene for BCMV Resistance with Scar Marker SW13 in Phaseolus vulgaris L. Generations, Derived from the Cross (BAT 477 X Dobroudjanski Ran)

Resistance to BCMV (Bean common mosaic virus) was established in Phaseolus vulgaris variety BAT 477, following artificial inoculation with the virus. Crosses were performed between BAT 477 and BCMV susceptible parent Dobrujanski ran, a widely spread Bulgarian variety, in order to transfer the resistance in the generations. By PCR, using SCAR marker SW 13, the presence of I-gene for BCMV resistance was proved in donor parent BAT 477, in all F1 plants and in segregating F2. The monogenic control of resistance was demonstrated by 690 bp fragment amplification in 75 plants and absent of fragment in the remaining 31 plants. The ratio of segregation was approximately 3:1, as evidenced by the criterion “χ2" at level of significance 0,50>P>0,20

Keywords:

Bean common mosaic virus (BCMV), I-gene Phaseolus vulgaris L,

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