Farklı Sıcaklık ve pH’ın Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne’nın Aksillar Sürgün Rejenerasyonu Üzerine Etkisi

Bitki doku kültürü çalışmalarında sıcaklık ve pH önemli faktörlerdir. Bu faktörlerin optimizasyonu çoklu bitki üretimi için önemlidir. Bu çalışmada, farklı sıcaklık (15-30°C) ve pH (5.5-6.6) koşullarında Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne’nın etkili ve hızlı üretimi için bir optimizasyon çalışması amaçlanmıştır. Sıcaklık çalışmalarında, ilk sürgün rejenerasyonları 25°C’de 12. günde gözlenmiştir. En yüksek sürgün rejenerasyon frekansları (%100) 20 ve 25°C’de elde edilmiştir. Maksimum eksplant başına sürgün sayısı (23.38 adet) ve en uzun sürgünler (1.74 cm) 25°C’de elde edilmiştir. Farklı pH uygulamalarında, en yüksek sürgün rejenerasyon oranları (%100) pH 5.5 ve 6’da tespit edilmiştir. En fazla eksplant başına sürgün sayısı (20.44 adet) ve en uzun sürgünler (1.71 cm) pH 6’da kaydedilmiştir. Eksplantlar genellikle yüksek pH değerlerinde daha uzun sürgünler vermiştir. Kültür ortamında üretilen sürgünler köklendirildikten sonra akvaryum ortamına başarıyla alıştırılmıştır. Sonuç olarak, R. rotundifolia’nın in vitro üretimi için optimum değerler 25°C sıcaklık ve pH 6 olarak bulunmuştur.

Anahtar Kelimeler:

Doku kültürü, pH, sıcaklık, sürgün rejenerasyonu

The Effect of Different Temperature and pH on Axillary Shoot Regeneration of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne

Temperature and pH are important factors in plant tissue culture studies. The optimization of these factors is important for multiple production of plants. In this study, an optimization study for the efficient and rapid production of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne in different temperature (15-30°C) and pH (5.5-6.6) conditions was aimed. In temperature studies, the first shoot regeneration was observed at 25°C on the 12th day. The highest shoot regeneration frequencies (100%) were obtained at 20 and 25°C. The maximum number of shoots per explant (23.38) and the longest shoots (1.74 cm) were obtained at 25 °C. In different pH applications, the highest shoot regeneration rate (100%) was determined at pH 5.5 and 6. The maximum number of shoots per explant (20.44) and the longest shoot (1.71 cm) were recorded at pH 6. The explants usually gave longer shoots at higher pH values. After the shoots produced in the culture medium were rooted, they were successfully used in the aquarium environment. As a result, the optimum values for in vitro production of R. rotundifolia were found to be 25°C temperature and pH 6.0.

Keywords:

Tissue culture, pH, temperature, shoot regeneration,

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