Elif Canpolat, Cemil İşlek, Ilgaz Akata, Şükrü Canpolat, Yasemin İşlek

Total Phenolics, Antioxidative and Antimicrobial Activities of Some Edible Ascomycota Collected from Niğde

Mushrooms have been used as food and medicinal purposes for centuries. In this study, some edible Ascomycota were collected in 2018 and 2019 from Niğde, Turkey. Molecular identifications of the samples were performed afterward. PCR products were sent for sequence analysis after ITS fragments were amplified with suitable primers concerning DNA isolation of samples. The obtained data were compared with the Genbank database for samples’ determination. The mushroom samples were determined to be Terfezia claveryi Chatin and Morchella esculenta (L.) Pers. Samples were extracted by ethanol and methanol to determine antimicrobial activity using the disc diffusion method. Antimicrobial zones against microorganisms were measured. Then DPPH radical scavenging effects and total phenolic content of T. claveryi and M. esculenta mushroom samples were studied. The highest antimicrobial activity was observed in ethanol extracts of M. esculenta. When the DPPH radical scavenging activities are taken into account, the activity is increased depending on the concentration. The highest DPPH scavenging activity was found in the extract of M. esculenta with 40.86%. It was determined that the total phenolic substances in methanol extracts of mushroom samples varies between 533.28 ± 1,15-537.34 ±2.20 μg GAE / mg extract.

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Acay H. 2018. Assessment Of The Nutritional Qualities And Bioactive Properties of The Wild Edible Mushroom Morchella esculenta (L.) Pers. Mantar Dergisi, 9: 95–105.
Akpi UK, Odoh CK, Ideh EE, Adobu US. 2017. Antimicrobial activity of Lycoperdon perlatum whole fruit body on common pathogenic bacteria and fungi. African Journal of Clinical and Experimental Microbiology, 18(2): 79-85.
Akyüz M. 2013. Nutritive value, flavonoid content and radical scavenging activity of the truffle (Terfezia boudieri Chatin). Journal of soil science and plant nutrition, 13(1): 143-151.
Akyüz M, Kireçci ADÖ, Gökçe Z, Kırbağ S, Yılmz Ö. 2019. Biochemical constituents and antioxidant activities of some mushrooms from Turkey: Agaricus spp., Pleurotus spp., Morchella esculenta and Terfezia boudieri. İstanbul Journal of Pharmacy, 49(1): 1-6.
Badalyan S. 2012. Medicinal aspects of edible ectomycorrhizal mushrooms. In Edible Ectomycorrhizal Mushrooms (pp. 317- 334). Springer, Berlin, Heidelberg.
Badalyan SM, Barkhudaryan A, Rapior S. 2019. Recent progress in research on the pharmacological potential of mushrooms and prospects for their clinical application. Medicinal Mushrooms, 1-70.
Badotti F, de Oliveira FS, Garcia CF, Vaz ABM, Fonseca PLC, Nahum LA, Oliveira G, Góes-Neto A. 2017. Effectiveness of ITS and sub-regions as DNA barcode markers for the identification of Basidiomycota (Fungi). BMC Microbiology, 17(1): 1-12.
Bajpai VK, Baek KH, Kang SC. 2017. Antioxidant and free radical scavenging activities of taxoquinone, a diterpenoid isolated from Metasequoia glyptostroboides. South African Journal of Botany, 111, 93-98.
Bal C, Sevindik M, Akgul H, Selamoglu Z. 2019. Oxidative stress index and antioxidant capacity of Lepista nuda collected from Gaziantep/Turkey. Sigma, 37(1): 1-5.
Boonsong S, Klaypradit W, Wilaipun P. 2016. Antioxidant activities of extracts from five edible mushrooms using different extractants. Agriculture and Natural Resources, 50(2): 89-97.
Breitenbach, J. and Kränzlin, F. 1984. Fungi of Switzerland, Vol: 1, Ascomycetes, Verlag Mykologia CH-6000 Luzern 9, 310 p., Switzerland.
Canlı K, Benek A, Şenturan M, Akata I, Altuner EM. 2019. In vitro Antimicrobial Activity of Morchella esculenta and Trametes versicolor. Mantar Dergisi, 10(3): 28-33.
Dahham SS, Al-Rawi SS, Ibrahim AH, Majid ASA, Majid AMSA. 2018. Antioxidant, anticancer, apoptosis properties and chemical composition of black truffle Terfezia claveryi. Saudi Journal of Biological Sciences, 25(8): 1524-1534.
Doğan HH, Aydin S. 2013. Determination of antimicrobial effect, antioxidant activity and phenolic contents of desert truffle in Turkey. African Journal of Traditional, Complementary and Alternative Medicines, 10(4): 52-58.
Doğan HH, Duman R, Özkalp B, Aydin S. 2013. Antimicrobial activities of some mushrooms in Turkey. Pharmaceutical Biology, 51(6): 707-711.
Duman R, Doğan HH, Ateş A. 2003. Morchella conica (Pers.) Boudier ve Suillus luteus (L.) SF Gray Makrofunguslarının Antimikrobiyal Aktiviteleri. Selçuk Üniversitesi Fen Fakültesi Fen Dergisi, 1(22): 19-24.
Eraslan EC, Altuntas D, Baba H, Bal C, Akgül H, Akata I, Sevındık M. 2021. Some Biological Activities and Element Contents of Ethanol Extract of Wild Edible Mushroom Morchella esculenta. Sigma: Journal of Engineering and Natural Sciences, 39(1): 24-28.
Giusti A, Ricci E, Gasperetti L, Galgani M, Polidori L, Verdigi F, Narducci R, Armani A. 2021. Molecular identification of mushroom species in Italy: An ongoing project aimed at reinforcing the control measures of an increasingly appreciated sustainable food. Sustainability, 13(1): 238(1-8).
Gücin F. Dülger B. 1997. Yenen ve Antimikrobiyal Aktiviteleri Olan Keme Mantarı (= terfezia boudieri chatin) Üzerinde Araştırmalar. Ekoloji, 6(23): 27-33.
Hussain G, Al-Ruqaie I.M. 1999. Occurrence, chemical composition, and nutritional value of truffles: an overview, Pakistan Journal of Biological Sciences, 2: 510- 514.
İnci Ş, Kırbağ S. 2018. Terfezia claveryi Chatin’in besinsel içeriği, antioksidan ve antimikrobiyal aktivitesi. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi, 19(2): 138-143.
Islek C, Saridogan BGO, Sevindik M, Akata I. 2021. Biological Activities and Heavy Metal Contents of Some Pholiota Species. Fresenius Environmental Bulletin, 30(06): 6109- 6114.
Janakat S, AlFakhiri S, Sallal A.K. 2004. A promising peptide antibiotic from Terfezia claveryi aqueous extract against Staphylococcus aureus in vitro. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives, 18(10): 810-813.
Kıvrak İ. 2015. Analytical methods applied to assess chemical composition, nutritional value and in vitro bioactivities of Terfezia olbiensis and Terfezia claveryi from Turkey. Food Analytical Methods, 8(5): 1279-1293.
Kim MY, Seguin P, Ahn JK, Kim JJ, Chun SC, Kim EH, Seo SY, Kang EY, Kim SL, Park YJ, Ro HM, Chung, I. M. 2008.
Phenolic compound concentration and antioxidant activities of edible and medicinal mushrooms from Korea. Journal of Agricultural and Food Chemistry, 56(16). 7265-7270.
Krupodorova T, Sevindik M. 2020. Antioxidant potential and some mineral contents of wild edible mushroom Ramaria stricta. AgroLife Scientific Journal, 9(1): 186-191.
Lindequist U, Niedermeyer TH, Jülich WD. 2005. The pharmacological potential of mushrooms. Evidence-based complementary and alternative medicine, 2(3): 285-299.
Rathee S, Rathee D, Rathee D, Kumar V, Rathee P. 2012. Mushrooms as therapeutic agents. Revista Brasileira de Farmacognosia, 22: 459-474.
Román MPG, Mantilla NB, Flórez SAC, De Mandal S, Passari AK, Ruiz-Villáfan B, Rodríguez-Sanoja R, Sánchez S. 2020. Antimicrobial and Antioxidant Potential of Wild Edible Mushrooms. In An Introduction to Mushroom (pp. 1-18). IntechOpen.
Saddiq AA, Danial EN. 2012. Assessment of phenolic content, free radical-scavenging capacity and antimicrobial activities of Truffle clavery. Wulfenia Journal, 19: 403-422.
Saridogan BGO, Islek C, Baba H, Akata I, Sevindik M. 2021. Antioxidant Antimicrobial Oxidant and Elements Contents Of Xylarıa Polymorpha And X. Hypoxylon (Xylariaceae). Fresenius Environmental Bulletin, 30(5): 5400-5404.
Schoch CL, Seifert KA, Huhndorf S, Robert V, Spouge JL, Levesque CA, Chen W, Fungal Barcoding Consortium. 2012. Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proceedings of the National Academy of Sciences, 109(16): 6241-6246.
Sevim E, Sevim A. 2021. Kırşehir İlinden Toplanan Morchella esculenta (L.) Pers (Kuzu Göbeği) Mantarlarının Moleküler Karakterizasyonu ve Antimikrobiyal Aktivitelerinin Belirlenmesi. Journal of Advanced Research in Natural and Applied Sciences, 7(1): 48-59.
Sevindik M, Akgül H, Günal S, Doğan M. 2016. Pleurotus ostreatus’un doğal ve kültür formlarının antimikrobiyal aktiviteleri ve mineral madde içeriklerinin belirlenmesi. Kastamonu University Journal of Forestry Faculty, 16(1): 153-156
Sevindik M. 2020. Antioxidant and antimicrobial capacity of Lactifluus rugatus and its antiproliferative activity on A549 cells. Indian Journal of Traditional Knowledge (IJTK), 19(2): 423-427.
Sevindik M, Akgul H, Korkmaz AI, Sen I. 2018. Antioxidant potantials of Helvella leucomelaena and Sarcosphaera coronaria. J Bacteriol Mycol Open Access, 6(2): 00173.
Sevindik M, Akata I. 2019. Antioxidant, oxidant potentials and element content of edible wild mushroom Helvella leucopus. Indian Journal of Natural Products and Resources, 10(4): 266-271.
Shimada K, Fujikawa K, Yahara K, Nakamura T. 1992. Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. Journal of agricultural and food chemistry, 40(6): 945-948.
Singleton VL, Orthofer R, Lamuela-Raventós RM. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in enzymology, 299: 152-178.
Taşkın H, Süfer Ö, Attar ŞH, Bozok F, Baktemur G, Büyükalaca S, Kafkas NE. 2021. Total phenolics, antioxidant activities and fatty acid profiles of six Morchella species. Journal of Food Science and Technology, 58(2): 692-700.
Vamanu E. 2014. Antioxidant properties of mushroom mycelia obtained by batch cultivation and tocopherol content affected by extraction procedures. BioMed Research International, doi.org/10.1155/2014/974804.
Vamanu E. Nita S. 2013. Antioxidant capacity and the correlation with major phenolic compounds, anthocyanin, and tocopherol content in various extracts from the wild edible Boletus edulis mushroom. BioMed Research International, doi.org/10.1155/2013/313905.
Vu D, Groenewald M, De Vries M, Gehrmann T, Stielow B, Eberhardt U, Al-Hatmi A, Groenewald JZ, Cardinali G, Houbraken J, Boekhout T, Crous PW, Robert V, Verkley GJM. 2019. Large-scale generation and analysis of filamentous fungal DNA barcodes boosts coverage for kingdom fungi and reveals thresholds for fungal species and higher taxon delimitation. Studies in mycology, 92: 135-154.
Wagay JA, Nayik GA, Wani SA, Mir RA, Ahmad MA, Rahman QI, Vyas D. 2019. Phenolic profiling and antioxidant capacity of Morchella esculenta L. by chemical and electrochemical methods at multiwall carbon nanotube paste electrode. Journal of Food Measurement and Characterization, 13(3): 1805-1819.
Wahiba B, Wafaà T, Asmaà K, Bouziane A, Mohammed B. 2016. Nutritional and antioxidant profile of red truffles (Terfezia claveryi) and white truffle (Tirmania nivea) from southwestern of Algeria. Der Pharmacia Letter, 8: 134-141.
White TJ, Bruns T, Lee SJWT, Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: a guide to methods and applications, 18(1): 315-322.