Forty seed sample of wheat (Triticum aestivum) were collected from four locations viz. Chitwan, Kaski, Banke and Lalitpur and tested by blotter method at laboratory during 2013 for determining fungal pathogens associated with wheat seeds in Nepal. Eighteen species representing thirteen genera of fungi were recovered from the seed. Alternaria alternata and Bipolaris sorokiniana were predominant in all the varieties/genotypes from all the locations, where B. sorokiniana was strongly pathogenic in wheat crop. Percentage frequency and type of fungi detected varied with variety and locations. Bipolaris sorokiniana was highest (64.40%) in Banke than remaining three locations. Seeds of Chitwan had lowest percentage (5.50%) of seed infection as compared to other locations. Relative abundance of Alternaria alternata (55.10%) was highest as it was the most prevalent component of seed borne mycoflora, followed by Bipolaris sorokiniana (34.69%) and Cladosporium herbarum (7.19%). Differences in quantity of precipitation and relative humidity might be the possible reason for variation in frequency and type of fungi detected in wheat seeds of four locations.
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Alam KB, Saha NK. 1991. Helminthosporium leaf blight of wheat a new problem in Bangladesh. Paper presented at international Workshop on Helminthosporium blight. CIMMYT, Mexico, D. F. 12 pages.
Barabara K, Mona T, Estein S, Birgitte A. 2004. Alternaria and Fusarium in Norwegain grains reduced quality -a matched pair sample study. Int. J. Food Microbiology. 93: 51-62.
Bashir, M. Mani MA, Kutama AS. 2012. Seed-borne mycoflora of local and improved wheat (Triticum aestivum L.) cultivars in Kano, Nigeria. Bayero Journal of Pure and Applied Sciences. 5: 101-103.
Bateman GL, Kwasna H. 1999. Effects of number of winter wheat crops grown successively on fungal communities on wheat roots. Applied Soil Ecology. 13: 271-82.
Clear RM, and Patrick'Can SK. 1993. Prevalence of some seed borne fungi on soft white winter wheat seed from Ontario, Canada. Plant Disease Survey. 73: 143-149.
Dexter JE, Clear RM, and Preston KR. 1996. Fusarium head blight: effect on the milling and baking of some Canadian wheats. Cereal Chemistry. 73: 695-701.
Duveiller E, Gilchrist L. 1994. Production constraints due to Bipolaris sorokiniana in wheat: Current situation and future prospects. In: Saunders DA, Hettel GP (ed.) Wheat in Heat- Stressed Environments: Irrigated, DryAreas and Rice-Wheat Systems. CIMMYT, Mexico D.F. pp. 343-352.
Fakhrunnisa, M. H. Hashmi and A. Ghaffar. 2006. Seed-borne mycoflora of wheat, sorghum and barley. Pakistan Journal of Botany. 38: 185-192.
Friis-Hansen E. 1995. Seeds for African peasants: Peasants' needs and agricultural research - the case of Zimbabwe. The Nordic African Institute. 227 pages.
Hershman, D. E. 2011. Black "sooty" head mold of wheat.University of Kentucky. Plant Pathology Fact Sheet.
Khanal NP, Maharjan KL, Sapkota A. 2012. Technical efficiency in wheat seed production: A case study from terai region of Nepal. J. Int. Dev. coop., 19: 41-50.
Khanzada KA, Rajput MA, Shah GS, Lodhi AM, Mehboob F. 2002. Effect of seed dressing fungicides for the control of seed borne mycoflora of wheat. A. J. Pl. Sci., 1: 441-444.
Mathur SB, Kongsdal O. 2003. Common laboratory seed health testing methods for detecting fungi (1sted). ISTA.
MOAD. 2013. Selected indicators of Nepalese agriculture and population.
Nafula MM. 1997. Assessment of seed borne pathogens for some important crops in western kenya. KARI-Katumani, PO Box, 340-90100.
Naqvi SDY, Shiden T, Merhawi W, Mehret S. 2013. Identification of seed borne fungi on farmer saved sorghum (Sorghum bicolor L.), pearl millet (Pennisetum glaucum L.) and groundnut (Arachis hypogaea L.) seeds. Agr. Sci. Res. J., 3: 107-114.
Pickett LS, Pruitt JD. 2010. Non-chemical methods for controlling diseases in the home landscape and garden. Oklahoma cooperative extension fact sheets available on website: http://osufacts.okstate.edu
Rosyara UR. 2002. Physio-morphological traits associated with resistance and tolerance to Helminthosporium leaf blight in spring wheat. M. Sc. Thesis, Tribhuvan University, Department of Plant Breeding, IAAS, Chitwan, Chitwan, Nepal. P. 2.
Saari EE. 1998. Leaf blight disease and associated soil borne fungal pathogens of wheat in South and Southeast Asia. In: Duveiller E, Dubin HJ, Reeves J, McNab A(ed.) Helminthosporium Blights of Wheat: Spot Blotch and Tan Spot. CIMMYT, Mexico D.F. pp. 37-51.
Salgado JD, Wallhead M, Madden LV, Paul PA. 2011. Grain harvesting strategies to minimize grain quality losses due to Fusarium head blight in wheat. Plant Dis. 95: 1448-1457.
Suproniene S, Mankeviciene A, Kadziene G. 2011.The effect of different tillage-fertilization practices on the mycoflora of wheat grains. Agricultural and Food Science. 20:315-326.
Vrandecic K, Jug D, Cosic J, Stosic M, Ilic J. 2013. The impact of tillage and fertilization on wheat grain infection. In: V. Vukadinovic and B. İur?evic (ed.) proceedings of 2nd International Scientific Conference on Soil and Crop Management: Adaptation and Mitigation of Climate Change, 26-28 Sept.2013. Osijek, Hrvatska. pp. 296-301.
Warham EJ, Butler LD, Sutton BC. 1996. Seed testing of maize and wheat: A laboratory guide. CIMMYT, Mexico. P. 84.
Wiese MV. 1987. Compendium of Wheat Diseases (2nded). The American Phytopathological Society, St Paul. USA.