Sıvı Kültürde Lepista Nuda Tarafından Miselyal Biyokütle ve Ekzopolisakkarit Üretimi İçin Karbon ve Azot Kaynaklarının Belirlenmesi

Bu çalışmada, sıvı kültürde ekzopolisakkarit üretimi için yenilebilir bir mantar olan Lepista nuda kullanılmıştır. Başlangıç karbon ve azot kaynaklarının L. nuda IT suşu tarafından ekzopolisakkarit ve biyokütle üretimi üzerindeki etkileri araştırılmıştır. En fazla ekzopolisakkarit üretimi 50 g/L glukoz ve 5 g/L maya ekstraktı kullanıldığında elde edilmiştir. Sonuç olarak, küçük ölçekte optimize edilmiş koşullar altında 2,1 g/L ekzopolisakkarit ve 12,3 g/L biyokütle üretimi sağlanmıştır. Bu çalışma, L. nuda'nın sıvı kültürde ekzopolisakkarit üretimini gösteren ilk çalışmadır.

Determination of Carbon and Nitrogen Sources for the Production of Mycelial Biomass and Exopolysaccharide by Lepista Nuda in Liquid Culture

In the present study, Lepista nuda, an edible mushroom, was used for the production of exopolysaccharide in the submerged culture. The effects of initial medium carbon and nitrogen sources on exopolysaccharide and mycelial biomass production by L. nuda IT strain were investigated. The highest exopolysaccharide production was obtained when culture parameters were used as initial medium 50 g/L glucose and 5 g/L yeast extract. As a result, 2.12 g/L exopolysaccharide and 12.3 g/L biomass production in optimized conditions at flask scales were achieved. This is the first study showing the production of exopolysaccharide in liquid culture of Lepista nuda.

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