Savrun Ispanak (Spinacia oleracea L.) Çeşidinin Bazı Abiyotik Stres Faktörlerine Tepkisi

Bu çalışmada Savrun ıspanak (Spinacea oleracea L.) çeşidinde tuz, ağır metal, kuraklık ve kireç stresine dayanıklık mekanizması araştırılmıştır. Bu amaçla kontrollü koşullarda iklim dobında yetiştirilen, 5-6 yapraklı fideler dört hafta süresince tuz (75, 150 ve 225 mM NaCl), ağır metal (%0,2 FeCl3, NiCl2 ve ZnCl2), kurak (%50) ve kireç (%0,2 CaCO3) stres uygulamalarına maruz bırakılmıştır. Çalışma sonucunda Savrun ıspanak çeşidinde klorofil a, b, toplam klorofil ve karotenoit, ?-karoten ve likopen içeriği kuraklık ve CaCO3 stres uygulamalarında artış göstermiştir. Toplam çözünür protein ve GuPX tüm stres uygulamalarında, prolin NiCl2, 75 ve 150 mM NaCl uygulamalarında yüksektir. APX aktivitesi 75 mM NaCl ve ZnCl2; CAT 150 mM NaCl ve kuraklık hariç diğer stres uygulamalarda; SOD aktivitesi ise kuraklık, CaCO3 ve 225 mM NaCl stres uygulamalarında yüksek bulunmuştur. MDA içeriği ise FeCl3 hariç diğer uygulamalarda düşük ve H2O2 ise 225 mM NaCl ve kuraklık uygulamasında düşük diğer uygulamalarda yüksektir. Sonuç olarak Savrun ıspanak çeşidinin CaCO3 ve kuraklık uygulamalarına toleransı yüksek, 225 mM NaCl, ZnCl2 ve FeCl3 uygulamalarına ise duyarlığı yüksek bulunmuştur. Çeşidin NiCl2 ve 75 mM NaCl uygulamalarına ise orta derecede toleranslı olduğu tespit edilmiştir. Bununla birlikte strese tolerans parametreleri oldukça değişkenlik göstermiştir

Effect of Some Abiotic Stress Factories on Savrun Spinach (Spinacea oleracea L.)

In this study were investigated that resistance to salinity, heavy metals, drought and calcerous stress in Savrun (Spinacea oleracea L.) spinach. For this aim, 5-6 leafed seedlings were exposed to NaCl (75, 150 and 225 mM); heavy metals (Fe, Ni and Zn 0.2 mg/L), drought (50%) and 0.2% CaCO3 applications for four weeks half-weekly which plants grown under controlled conditions. Depends on result chlorophyll a, chlorophyll b, total cclorophyll, carotenoids, ?-caroten and lycopen increased in drought and CaCO3 treatments. Total soluble protein and GuPX activity were found higher in all stress treatments, proline content increased in NiCl2, 75 ve 150 mM NaCl treatments. While APX activity was higher in 75 mM NaCl and ZnCl2; CAT was higher in 150 mM NaCl and others stres treatments except drought. SOD activity were noted higher in drought, CaCO3 and 225 mM NaCl stresses groups. MDA content was lower in all treatments except FeCl3 and, H2O2 were lower in 225 mM NaCl and drought while it was higher in others. As a result, it was found that tolerance of Savrun spinach is higher to CaCO3 and drought but sensitivity of it is higher to 225 mM NaCl, ZnCl2 and FeCl3. Also it was determined that Savrun genotype is moderate tolerance to NiCl2 and 75 Mm NaCl. However parameters of tolerance to stress that treated on spinach seedling showed variability in Savrun spinach.

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Aksu E, Yıldız N. 2004. Heavy Metal Stress and Tolerance of Plants. International Soil Congress on Natural Resource Management for Sustainable Development. Erzurum.
Arnon DI. 1949. Copper enzymes in isolated chloroplast. Polyphenoloxidase in Beta vulgaris. Plant Physiology, 24: 1-10.
Bates LS, Waldern RP, Teare ID. 1973. Rapid determination of free proline for water-stress studies. Plant and Soil, 39: 205- 207.
Bergmeyer HU. 1974. Methods of Enzymatic Analysis. New York, Academic Press. Biol. Plant. 39: 303-308
Bergquist AM, Gertsson UE, Nordmark YG, Olsson ME. 2007. Ascorbic Acid, Carotenoids, and Visual Quality of Baby Spinach as Affected by Shade Netting and Postharvest Storage. J. Agric. Food Chem. 55: 8444-8451.
Bolwell GP, Bindschedler LV, Blee KA, Butt, VS, Davies, DR, Gardner SL, Gerrish C, Minibayeva F. 2002. The apoplastic oxidative burst in response to biotic stress in plants: a tree component system. J. Exp. Bot. 53: 1367-1376.
Bradford MM. 1976. A Rapid and Sensitive Method for the Quantition of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding. Anal. Biochem. 72: 248.
Briemer T. 1982. Environmental factors and culture measures affecting the nitrate content of spinach. Fertility Residual. 3: 191-291.
Chance B, Maehly SK. 1995. Assay of catalase and peroxidase. Methods Enzymol. 2: 764-775.
Cornic G, Fresneau C. 2002. Photosynthetic carbon reduction and oxidation cycles are the main electron sinks for photosystem II activity during a mild drought. Ann. Bot. 89: 887-94.
Cruz FJR, Castro, GLS, Silva JDD, Festucci-Buselli RA, Pinheiro HA. 2013. Exogenous glycine betaine modulates ascorbate peroxidase and catalase activities and prevent lipid peroxidation in mild water-stressed Carapa guianensis plants. Photosynthetica, 51: 102-108.
Çakmak İ. 2002. Plant nutrition Research: Priorities to Meet Human Needs for Food in Sustainable Ways. Plant and Soil, 247: 3-24.
Çiçek N, Çakirlar H. 2002. The Effect of Salinity on Some Physiol. Parameters in two Maize Cult..Bulg. J.Plant Physiol. 28(1–2): 6674.
Dhindsa RS, Plumb DP, Thorpe TA. 1981. Leaf senescence correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. J. Exp. Bot. 32: 93-101.
Doğan M, Tıpırdamaz R, Demir Y. 2010. Salt Resistance of Tomato Species Grown in Sand Culture, Plant Soil Environment, 56 (11): 499-507.
Doğanlar ZB, Cakmak S, Yanik T. 2012. Metal Uptake and Physiological Changes in Lemna gibba Exposed to Manganese and Nickel. I J Biol. 4(3): 148-157.
Dubey RS and Rani M. 1989. Influence of NaCl salinity on growth and metabolic status of protein amino acids in rice seedling. J. Agron. Crop Sc. 162: 97-106.
Dubey RS. 1994. Protein synthesis by plants under stressful conditions. pp.277-299. In: M. Pessarakli (Ed.), Handbook of Plant and Crop Stress, Macel Dekker Inc., New York.
Dürüst N, Dürüst Y, Tuğrul D, Zengin M. 2004. Heavy Metal Contents of Pinus Radiata Trees of İzmit (Turkey). Asian Journal of Chemistry, 16(2): 1129-1134.
El-Enany AE. 1997. Shoot regeneration and protein synthesis in tomato tissue cultures. Biol.Plant, 39: 303-308.
Elia A, Santamaria P, Serio F. 1999. Nitrogen Nutrition, Yield and Quality of Spinach. Journal of Food Science and Agriculture, 76: 341-346.
Ergün N, Mutlu A, Özçubuk S. 2011. Sıcaklık-Ağır Metal (Cr ve Cu) Etkileşimlerinin Buğday Fidelerinde Büyüme ve Katalaz Aktivitesi Üzerine Etkileri. C. U. Fen Fakültesi Fen Bilimleri Dergisi, Cilt 32, No:1.
Eyüpoğlu F, Kurucu N, Talaz S. 1998. Türkiye Topraklarının Bitkiye Yarayışlı Mikroelementler Bakımından Genel Durumu. T.C. Başbakanlık Köy Hizmetleri Genel Müdürlüğü, Toprak ve Gübre Araştırma Ens. Müd. Genel Yayın: 217, Seri No: R-133, Ankara.
Foyer CH, Shigeoka S. 2011. Understanding oxidative stress and antioxidant functions to enhance photosynthesis. Plant Physiology, 155: 93-100.
Galston AW, Kaur-Sawhney R, Atabella T, Tiburcio AF. 1999. Plant polyamines in reproductive activity and response to abiotic stress. Bot. Acta, 110: 197-207.
Halliwell B. 1987. Oxidative damage, lipid peroxidation and antioxidant protection in chloroplasts. Chem. Phys. Lipids, 44: 327-340.
Hernandez JA, Olmos E, Corpas FJ, Sevilla F, Del Rio IA. 1995. Salt-Induced Oxidative Stress in Chloroplasts of Pea Plants. Plant Sci. 105: 151-167.
Kansal BD, Singh B, Bajaj KL, Kaur G. 1981. Effect of Different Levels of Nitrogen and Farmyard Manure on Yield and Quality of Spinach (Spinacea oleracea L.). Qual. Plant. Plant Foods Hum. Nutr. 31: 163-70.
Kavas M, Baloğlu MC, Akça O, Köse F, Gökçay D. 2013. Effect of drought stress on oxidative damage and antioxidant enzyme activity in melon seedlings. Turkish Journal of Biology, 37: 491-498.
Keyvan S. 2010. The effects of drought stress on yield, relative water content, proline, soluble carbohydrates and chlorophyll of bread wheat cultivars. Journal of Animal & Plant Sciences, 8(3): 1051-1060.
Kim TH, Lee BR, Jung WJ, Kim KY, Avice JC, Qurry A. 2004. Denovo protein synthesis in relation to ammonia and proline accumulation in water stressed white clover. Funct. Plant Biol. 31: 847-855.
Koca H, Bor M, Özdemir F, Türkan İ. 2007. The effect of salt stress on lipid peroxidation, antioxidative enzymes and proline content of sesame cultivars. Environmental and Experimental Botany, 60: 344-351.
Koç E, Üstün SA, Arıcı YK. 2012. Biber (Capsicum annuum L.) Fidelerinde Farklı Çinko Konsantrasyonlarının Total Protein, Hidrojen Peroksit İçeriği ve Peroksidaz Aktivitesi Üzerine Etkisi. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi, 13(2): 205-212.
Kurnaz A, Mutlu E, Uncumusaoğlu AA. 2016. Determination of Water Quality Parameters and Heavy Metal Content in Surface Water of Çiğdem Pond (Kastamonu/Turkey).Turkish Journal of agriculture –Food Science and Technology, 4(10): 907-913.
Kurnaz A. 2016. First Detailed Measurements of Environmental Radioactivity and Radiation Hazard Assessment for Gerze- Turkey. Fresenius Environmental Bulletin, 25(1): 153-162.
Lamhamdi M, Galiou Q, Bakrim A, No´voa-Mun˜ oz JC, Arias- Este´vez M, Aarab A, Lafont R. 2013. Effect of lead stress on mineral content and growth of wheat (Triticum aestivum) and spinach (Spinacia oleracea) Seedlings. Saudi Journal of Biological Sciences, 20: 29-36.
Lee DH, Lee CB. 2000. Chilling Stres-induced Changes of Antioxidant Enzymes in the Leaves of Cucumber in Gel Enzyme Activity Assays. Plant Science, 159: 75-85.
Lutts S, Kinet JM, Bouharmont J. 1996. NaCl-Induced Senescence in Leaves of Rice (Oryza sativa L.) Cultivars Differing in Salinity Res istance. Annals of Botany, 78: 389- 398.
Manivasagaperumal R, Balamurugan S, Thiyagarajan G, Sekar J. 2011. Effect of Zinc on Germination, Seedling Growth and Biochemical Content of Cluster Bean (Cyamopsis tetragonoloba (L.) Taub). Curr. Bot. 2: 11-15.
McDonald S, Archbold D.1998. Membrane competence among and within Fragaria species varies in response to dehydration stress. J. Am. Soc. Hort. Sci. 123(5): 808-813.
Mittova V, Tal M, Volokitta M, Guy M. 2002. Salt Stres Induces Up-regulation of an Efficient Chloroplast Antioxidant System in the SAlt-tolerant wild Tomato Species Lycopersicon pennellii but not in the Cultivated Species. Physiologia Pantarum, 115: 393-400.
Mutlu E, Uncumusaoğlu AA. 2016. Kuruçay Deresinin Su Kalitesinin Fiziko- Kimyasal Analizi. Türk Tarm-Gıda Bilim ve Teknoloji Dergisi, 4(11): 991-998.
Nagata M, Yamashita I. 1992. Simple Method for Simultaneous Determination of Chlorophyll and Carotenoids in Tomato Fruit. Nippon Shokuhin Kogyo Gakkaish, 39 (10): 925-928.
Nakano Y, Asada K. 1981. Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant Cell Physiol. 21: 1295-1307.
Oidaire H, Sano S, Koshiba T, Ushimaru T. 2000. Enhancement of Antioxidative Enzyme Activities in Chilled Rice Seedlings. Journal of Plant Physiolgy, 156: 811-813.
Öncel I, Keleş Y. 2002. Tuz Stresi Altındaki Buğday Genotiplerinde Büyüme, Pigment İçeriği ve Çözünür Madde Kompozisyonunda Değişmeler. C.Ü. Fen-Edebiyat Fakültesi Fen Bilimleri Dergisi, 23(2): 9-16.
Pandey N, Pathak GC, Pandey KD, Pandey R. 2009. Heavy Metals, Co, Ni, Cu, Zn and Cd Produce oxidative damage and evoke differantial antioxidant response in spinasch. Braz. J. Plant. Physiol. 21(2): 103-111.
Pandey N, Pathak GC. 2006. Nickel alters antioxidative defense and water status in green gram. Indian J. Plant Physiol. 11: 113-118.
Parida AK, Das AB, Mittra B, Mohanty P. 2004. Salt-stress induced alterations in protein profile and protease activity in the mangrove, Bruguiera parviflora. Z. Naturforsch. 59: 408-414.
Pérez-López U, Robredo A, Lacuesta M, Sgherri C, Mu˜noz- Rueda A, Navari-Izzo F, Mena-Petite A. 2009. The oxidative stress caused by salinity in two barley cultivars is mitigated by elevated CO2. Physiol. Plant, 135: 29-42.
Polesskaya OG, Kashirina EI, Alekhina ND. 2006. Effect of Salt Stress on Antioxidant System of Plants as Related to Nitrogen Nutrition, Russian Journal of Plant Physiology, 53(2): 186-192.
Prasad DAG, Rahimpouran S, Komala HP. 2014. Ecotoxicological effects of iron on the activities of antioxidant enzymes in Safflower (Carthamus tinctorius L.) seedlings. Int. J. Pure App. Biosci. 2(5): 118-123.
Rastgoo L, Alemzadeh A, Tale MA, Tazangi SE, Eslamzadeh T. 2014. Effects of copper, nickel and zinc on biochemical parameters and metal accumulation in gouan, Aeluropus littoralis. Plant Knowledge Journal, 3(1): 31-38. ESSN: 2200-5390 Australia.
Shalata A, Mittova V, Volokita Guy M, Tal M. 2001. Response of the Cultivated Tomato and its Wild Salt-Tolerant Relative Lycopersicon pennelli to Salt-Dependet Oxidative Stres: The Root Antioxidative System. Physiologia Plantarum, 112: 487-494.
Singh Y, Malik CP. 2011. Phenols and their antioxidant activity in Brassica juncea seedlings growing under HgCl2 stress. J. Microbiol. Biotech. Res. 1 (4): 124-130.
Smirnoff N. 2005. Ascorbate, Tocopherol and Carotenoids: Metabolism, Pathway Engineering and Functions. In: Smirnoff N, Ed. Antioxidants and Reactive Oxygen Species in Plants. Oxford: Blackwell Publishing Ltd. p:53-86.
Stobart AK, Griffiths WT, Amcen-Bukhari I, Sherwood,RP. 1985. The effect of Cd+2 on the biosynthesis of chlorophyll in leaves of barley. Physiol. Plant, 63: 293-298.
Sultana B, Aslam M, Anwar F, Munir H. 2016. Foliar spray of selected plant growth regulators affected the biochemical and antioxidant attributes of spinach in a field experiment. Turkish Journal of Agriculture and Forestry, 40: 136-145.
Toledo MEA, Ueda Y, Imahori Y, Ayaki, M. 2003. L-ascorbic acid metabolism in spinach (Spinacia oleracea L.) during postharvest storage in light and dark. Journal of Postharvest Biology and Technol. 28: 47-57.
Turfan N, Kurnaz A, Alay M, Sarıyıldız T. 2016. Determining of Some Chemical Properties in Taşköprü Garlic Stored in Different Conditions. Kastamonu University Journal of Forestry Faculty, 16 (2): 427-437.
Türkan İ, Bor M, Ozdemir F, Koca H. 2004. Differential responses of lipid peroxidation and antioxidants in the leaves of drought tolerant P. acutifolius gray and droughtsensitive P. vulgaris L. subjected to polyethylene glycol mediated water stress. Plant Sci. 168: 223-231.
Velikova V, Yordanov I, Edrava A. 2000. Oxidative stress and some antioxidant systems in acid rain-treated bean plants. Protective role of exogenous polyamines. Plant Science, 151: 59-66.
Vinod K, Awasthi G, Chauhan PK. 2012. Cu and Zn tolerance and responses of the Biochemical and Physiochemical system of Wheat. Journal of Stress Physiology & Biochemistry, 8(3): 203-213.
Wang L, Chen G, Zhang G, Li X, Ni S, Yang R. 2010. Water use efficiency of soybean under different mulching in dryland. Soybean Science, 9(5): 767-771.
Welch RM. 2002. The impact of mineral nutrients in food crops on global human health. Plant and Soil, 247: 83-90.
Witham FH, Blaydes DF, Devlin RM. 1971. Experiments in plant physiology, pp 55-56. Van Nostrand Reinhold Company, New York.
Yıldız M, Terzi H. 2008. Effects of NaCl on protein profiles of tetraploid and hexaploid wheat species and their diploid wild progenitors. Plant Soil Environ. 54: 227-233.
Zengin FK, Munzuroğlu Ö. 2005. Effects of some heavy metals on content of chlorophyll, proline and some antioxidant chemicals in bean (Phaseolus vulgaris L.) seedlings. Acta Biologica Cracoviensia, 47(2): 157-164.
Zengin FK, Munzuroğlu Ö. 2006. Ayçiçeği (Helianthus annuus L.) Fidelerinin Toplam Çözünebilir Protein, Prolin ve Klorofil Miktarları Üzerine Civa Klorürün (HgCl2) Etkileri. Fırat Üniv. Fen ve Müh. Bil. Der. 18 (1): 25-30.
Zheng Y, Liu Y, Guo K, Fan Dayong, Li Guoqing, Yu L, Yang R. 2011. Effect of drought on pigments, osmotic adjustment and antioxidant enzymes in six woody plant species in karst habitats of southwestern China Environmental and Experimental Botany, 71: 174-180.