Venkatachalam Balamurugan, Manikandan Sridhivya, Ramachandran Dharani, Subramaniam Selvakumar, Krishnan Vasanth

Phytochemical Screening, Antioxidant, Antidiabetic and Anticancer Activities of Elaeocarpus variabilis Fruit

This study focused on the determination of secondary metabolites to an evaluation of the biological activity of Elaeocarpus variabilis Zmarzty fruit extract, an endemic medicinal plant. Their total Phenolic, tannin, flavonoids, alkaloids and saponins contents were quantified and their antioxidant activity, determined by DPPH, total antioxidant, ABTS, FRAP and Fe2+ chelating activity. The ethyl acetate extract exhibited the highest radical scavenging activity. The most relevant contents in total phenolics (Gallic Acid Equivalent mg/g), tannin (Gallic Acid Equivalent mg/g), total flavonoids (Rutin equivalent mg/g), total alkaloids (Quinine equivalent mg/g), and total saponin (Diosgenin equivalent mg/g) were observed. Significant correlations were established between antioxidant activity and secondary metabolites. Though the in vitro anti-diabetic activity was carried out using -amylase and -glucosidase inhibition, this indicates that the absorption and digestion of carbohydrates would probably reduce the blood glucose levels and hence help the management of type-2 diabetes. The ethyl acetate extract exhibited the potential of anticancer activity on human HT-29 colon cancer cell line IC50 values (30.0 ± 1.0) the valuable activity that compared to doxorubicin (17.0 ± 0.5). While this is the first study concerning extract from E. variabilis, our findings highlighted the richness’ in flavonoids of those extracts and their amazing potential as a source of antioxidant, anti-inflammatory, anti-diabetic, and anticancer agents.

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postolidis E, Kwon YI, Shetty K. 2007. ‘Inhibitory potential of herb, fruit and fungal-enrichd cheese against key enzymes linked to type 2 diabetes and hypertension. Innovative Food Science and Emerging Technologies, 8(1): 46-54.
Aragaw HS, Nohr D,Callo-Concha D. 2021.Nutritional potential of underutilized edible plant species in coffee agroforestry systems of Yayu, southwestern Ethiopia. Agroforest Syst, 95: 1047–1059.
Arnao MB, Cano A, Acosta M. 2001. The hydrophilic and lipophilic contribution to total antioxidant activity. Food chemistry, 73(2): 239-244.
Arti N, Archana A. 2007. Lab Manual in Biochemistry, Immmunology and Biotechnology’. Tata MC Graw-Hill Publishing Company Limited, 1: 84-85.
Benzie IF, Strain JJ. 1996. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical biochemistry, 239(1): 70-76.
Benderitter M. 1998. Studies by Electron Paramagnetic Resonance of the Importance of Iron in the Hydroxyl Scavenging Properties of Ascorbic acid in plasma: Effects of Iron Chelators. Fundamental and Clinic Pharmacol, 12(5): 510-516.
Bharti A. 2013. Determination of Quercetin in extract of Elaeocarpus ganitrusRoxb seeds by using HPTLC method. International Reseach Journal of Pharmacy, 4(3): 186-188.
Bhatt BD,Dahal P. 2019. Antioxidant and Antimicrobial Efficacy of Various Solvent Extracts of Seed of Rudrakshya (Elaeocarpus ganitrus) from Ilam District of Nepal. Journal of Nepal Chemical Society, 40: 11-18.
Bhattacharya SK, Debnath PK, Pandey VB, Sanyal AK. 1975. Pharmacological in vestigation on Elaeocarpus ganitrus. Planta Medica,28(06): 174-177.
Braca A, Tommasi ND, Bari LD, Pizza C, Politi, Morelli I. 2001. Antioxidant principles from Bauhuniaterapotensis. Journal of Natural Products, 64(7): 892-895.
Carmichael J, DeGra WG, Gazdar AF, Minna JD, Mitchell JB. 1987. Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Researh, 47: 936- 942.
Cespedes CL, Eihafidi M, Pavon N, Alarcon J. 2010. Antioxidant and cardioprotective activities of phenolic extracts from fruits of Chilean blackberry Aristotelia chilensis(Elaeocarpaceae), Maqui. Food Chemisty, 107(2): 820–829.
Chen YH, Yang CY. 2020. Ultrasound-Assisted Extraction of Bioactive Compounds and Antioxidant Capacity for the Valorization of Elaeocarpus serratus L. Leaves. Processes,8(10): 1218.
Chopra RN. 1933. Indigenous Drugs of India. The Art Press. Calcutta, 58-76.
Chowdhury A, Azam S, Jainul MA, Faruq KO, Islam A. 2014. Antibacterial activities and in vitro anti-inflammatory (membrane stability) properties of methanolic extracts of Gardenia coronaria leaves. International Journal of Microbiology, 1-5.
Dinis TCP, Madeira VMC, Almeida LM. 1994. Action of Phenolic Derivatives (Acetaminophen, Salicylate and 5- Aminosalicylate) as Inhibitors of Membrane Lipid Per oxidation and as Peroxyl Radical Scavengers. Biophysics Journal, 315(1): 161-169.
El Omari N, Sayah K, Fettach S, El Blidi O, Bouyahya A, Faouzi MEA, Barkiyou M. 2019. Evaluation of in vitro antioxidant and antidiabetic activities of Aristolochia longa extracts. Evidence-Based Complementary and Alternative Medicine, 1-9.
Gandhisan R, Thamaraichelvan A, Baburaj. 1991.
Antiinflammatory action of Lannea coromandelica Hrbc membrane stabilization. Fitotherapia, 62(1): 81- 83.
Gaurav K, Karthik L, Rao KVB. 2014. A Review on Medicinal Properties of Elaeocarpus ganitrus Roxb.ex G. Don (Elaeocarpaceae). Research Journal of Pharmacy and technology, 7(10): 1184-1186.
Geetha DH, Rajeswari M,Indiramuthu J. 2013. Chemical profiling of Elaeocarpus serratus L. by GC-MS. Asian Pacific Journal of Tropical Biomedicine, 3(12): 985-987.
Gonzalez-Molina E, Domínguez-Perles R, Moreno DA, García- Viguera C. 2010. Natural bioactive compounds of Citrus limon for food and health. Journal of Pharmaceutical and Biomedical Analysis, 51(2): 327-345.
Hardainiyan S, Nandy BC, Saxena R. 2015. Phytochemical investigation of fruit extract of Elaeocarpus ganitrus. International Journal of Pharmacy and Pharmaceutical Sciences, 7(6): 415-418.
Havsteen B. 1983. Flavonoids, a class of natural products of high pharmacological potency. Biochemical pharmacology, 32(7): 1141-1148.
Hendrickson BA, Gokhale R, Cho JH. 2002. Clinical aspects and pathophysiology of inflammatory bowel disease. Clinical Microbiology Reviews, 15(1): 79-94.
Hodge JE,Hofreiter BT. 1962. Determination of reducing sugar and carbohydrates. In: methods in carbohydrate chemistry, whistler, RL, ML wolfram, JN Be-Miller and F Shafizadeh (Eds.). Academic Press Inc, New York USA, pp: 380-394.
Jain SK. 1991. Dictionary of Indian folk medicine and ethnobotany. New Delhi: Deep publication,53: 75 -82.
Jawla S, Rai DV. 2016. Pharmacognostic Studies on Rudraksh (Elaeocarpus angustifolius Blume) Fruit. Advances in Biological Research, 10(6): 382-387.
Jayashree I, Geetha DH, Rajeswari M. 2019. GC-MS Analysis of Ethanolic Extract of Leaf of Elaeocarpus tuberculatusRoxb. International Journal of Pharmacognosy and Phytochemical Research, 11: 299-303.
Joshi S, Gupta P, Kumar N, Rai N, Gautam P, Thapliyal A. 2012. A comprehensive report on therapeutic potential of Elaeocarpus ganitrus Roxb.(Rudraksha). Environment Conservation Journal, 13(3): 147-150.
Khan ML, Bhuyan P, Tripathi RS. 2003. Regeneration of Rudraksh (Elaeocarpus ganitrus Roxb.) – a threatened tree species: germination strategies. International Journal of Ecology and Environmental Science, 29: 255–260.
Korkmaz N, Dayangac A, Sevindik M. 2021. Antioxidant, antimicrobial and antiproliferative activities of Galium aparine. Journal of Faculty of Pharmacy of Ankara University, 45(3): 554-564.
Krishnan V, Subramaniam S, Chia–Chuan C, Venkatachalam B, Thomas Cheeran A, Chi-Ying FH. 2020. Anticancer Activity of Leonurus sibiricus L.: Possible Involvement of Intrinsic Apoptotic Pathway. Nutrition and Cancer, 1-12.
Kumari N, Atri NS. 2014. Nutritional and Nutraceutical Potential of Wild Edible Macrolepiotoid Mushroom of North India. International Journal of Pharmacy and Pharamaceutical Science, 6(2): 200-204.
Kumar A, Kumar M, Verma RK, Kumar A, Punar S, Ram L, Maheshwari RK. 2021. A Comprehensive Review on Phytochemical, Pharmacological, Dielectric and Therapeutic Attributes of Multifarious Rudraksha (Elaeocarpus Ganitrus Roxb.). Applied Sciences, 9(1): 97-109.
Lakshmi MA, Srinivas K, Rani SU,Praneetha V. 2016. Evaluation of in vitro anti inflammatory activity of Elaeocarpus ganitrus of bark extract by hrbc membrane stabilization. International Journal of Pharmaceutical Chemical and Biological Sciences,6(4).
Lakshmi SR, Kumar TS, Rao MV. 2014. Phytochemical Analysis, Histochemical localization and Antioxidant Activity of Hoya wightii ssp. palniensis and Elaeocarpus recurvatus. American Journal of Phytomedicine and Clinical Therapeutics, 2(3): 57-366.
Lonkisch M, Layer P, Rizza RA, Magno EP. 1998. Acute post prandial gastrointestinal and metabolic effects of wheat amylase inhibitor (WAI) in normal, obsess and diabetic humans. Pancreas,17(2): 176-181.
Lowry OH, Rosebrough NJ, Farr AL, anandall RJ. 1951. Protein measurement with the Folin phenol reagent. Journal of biological chemistry, 193: 265-275.
Lu B, Liu L, Zhen X, Wu X, Zhang Y. 2010. Anti-tumor activity of triterpenoid-rich extract from bamboo shavings (Caulis bambusae in Taeniam). African Journal of Biotechnology, 9(38): 6430-6436.
Makkar HP, Siddhuraju P, Becker K. 2007. Plant secondary metabolites, Totowa: Humana Press Inc, p93-100.
Makkar HPS. 2003. Quantification of Tannins in Tree and Shrub Foliage, Makkar, H.P.S., (ed.). Kluwer Academic Press. New York,pp. 102.
Manoharan AL, Thamburaj S, Muniyandi K, Jagadeesan G, Sathyanarayanan S, Nataraj G,Thangaraj P. 2019.
Antioxidant and antimicrobial investigations of Elaeocarpus tectorius (Lour.) Poir. fruits against urinary tract infection pathogens. Biocatalysis and Agricultural Biotechnology, 20: 101260.
Matsuda H, Kagerura T, Toguchida I, Ueda H, Morikawa T, Yoshikawa M. 2000. Inhibitory effects of sesquiterpenes from bay leaf on nitric oxide production in lipopolysaccharide-activated macrophages: Structure requirement and role of heat shock proteininduction. Life Science, 66(22): 2151-2157.
Meenakshi VK, Gomathi S, Senthamarai S, Paripooranaselvi M, Chamundeswari KP. 2012. Analysis of vitamins by HPLC and phenolic-compounds, flavonoids by HPTLC in Microcosmus-exasperatus. European Journal of Zoological Research, 1(4): 105-110.
Mohammed FS, Günal S, Pehlivan M, Doğan M, Sevindik M, Akgül H. 2020. Phenolic content, antioxidant and antimicrobial potential of endemic Ferulago platycarpa. Gazi University Journal of Science, 33(4): 670-677.
Mohammed FS, Pehlivan M, SevindikE, Akgul H, Sevindik M, Bozgeyik I, Yumrutas O. 2021. Pharmacological properties of edible Asparagus acutifolius and Asparagus officinalis collected from North Iraq and Turkey (Hatay). Acta Alimentaria, 50(1): 136-143.
Moore S, Stein WH. 1948. In methods in enzymol. Academic Press. New York, 3, 468.
Nayan talukdar N, Dutta AM,Karabi D. 2016. Anti-Oxidant, Antimicrobial and Inhibitory effect on α-Amylase of Ethyl acetate extracts from the bark of Elaeocarpus ganitrus.Indo American Journal of Pharmaceutical Research, 6: 6926-6931.
Neeld JB, Pearson WN. 1963. Macro and Micro Methods for the Determination of Serum Vitamin A using Trifluoroacetic acid. The Journal of nutrition, 79(4): 454-462.
Okselni T, Santoni A, Dharma A,Efdi M. 2019. Biological activity of methanol extract of Elaeocarpus mastersii king: antioxidant, antibacterial, and α-glucosidase inhibitor. Rasayan Journal of Chemistry, 12(1): 146-151.
Okwu DE, Ndu CU. 2006. Evaluation of the phytonutrient, mineral and vitamin content of some varieties of yam (Discoreaspp). International Journal of Molecular Medicine and Advance Science, 2(2): 199-203.
Prieto P, Pineda M, Aguilar M. 1999. Spectrophotometric quantification of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application of vitamin-E. Analytical Biochemistry, 269(2): 337-341.
Prihantini AI, Tachibana S. 2017. Antioxidant compounds produced by Pseudocercospora sp. ESL 02, an endophytic fungus isolated from Elaeocarpus sylvestris. Asian Pacific Journal of Tropical Biomedicine, 7(2): 110-115.
Prihantini AI, Tachibana S, Itoh K. 2014. Evaluation of antioxidant and α-glucosidase inhibitory activities of some subtropical plants. Pakistan journal of biological sciences, 17(10): 1106-1114.
Raghunathan M. 2017. Assessment of Vitamins, Flavonoids And Phenolic-compounds by Hplc in the fruit Of Elaeocarpus Oblongus. European Journal of Pharmaceutical and Medical Research,4: 235-241.
Rajapaksha U. 1998. Traditional Food Plants in Sri Lanka. Hector Kobbekaduwa Agrarian Research and Training Institute. Sri Lanka, 231.
Ramful D, Tarnus E, Aruoma O I, Bourdon E,Bahorun T. 2011. Polyphenol composition, vitamin C content and antioxidant capacity of Mauritian citrus fruit pulps. Food Research International, 44(7): 2088-2099.
Razali N, Mat-Junit S, Abdul-Muthalib AF, Subramaniam S, Abdul-Aziz A. 2012. Effects of various solvents on the extraction of antioxidant phenolics from the leaves, seeds, veins and skins of Tamarindus indica L. Food Chemistry, 131(2): 441-448.
Roux GF, Perrier J, Forest E, MourenGM, Puigserver A, Santimone M. 2001. The human pancreatic alpha-amylase isoforms: isolation, structural studies and kinetics of inhibition by Acarbose.Asian Journal of Pharmaceutical Research,1388(1): 10-20.
Sadasivam S, Manickam A. 2004. Biochemical Methods, 2nd edition, New Age International (P) Ltd, Publishers, New Delhi.
Sakat S, Juvekar AR,Gambhire MN. 2010. In vitro antioxidant and anti-inflammatory activity of methanol extract of Oxalis corniculata Linn. International Journal of Pharmacy and Pharmaceutical Sciences, 2: 146-155.
Sareen A, Gupta RC, Bansal G, Singh V. 2020. Comparison of key mineral elements in wild edible fruits of Ziziphus mauritiana and Ziziphus nummularia using atomic absorption spectrophotometer (AAS) and flame photometer. International Journal of Fruit Science, 20(sup2): S987-S994.
Sathish Kumar T, Shanmugam S, Palvannan T, Bharathi Kumar VM. 2010. Evaluation of antioxidant properties of Elaeocarpus ganitrus Roxb leaves. Iranian Journal of Pharmaceutical Research, 3: 211-215.
Selamoglu Z, Sevindik M, Bal C, Ozaltun B, Sen İ, Pasdaran A. 2020. Antioxidant, antimicrobial and DNA protection activities of phenolic content of Tricholoma virgatum (Fr.) P.Kumm. Biointerface Research in Applied Chemistry, 10(3): 5500-5506
Sevindik M. 2021. Phenolic content, antioxidant and antimicrobial potential of Melanoleucamelaleuca edible mushroom. Journal of Animal & Plant Sciences, 31(3): 824-830
Shamna R, Sasikumar JM,Sugathakumar E. 2011. Antioxidant Capacity of Rubus niveusThunb. and Elaeocarpus oblongus Gaestn Fruits. Global science book, 5: 96-101.
Sharma A, Swati J,Navin K. 2015. Antioxidant and antibacterial properties of leaves of Elaeocarpus sphaericus Roxb. And Pinus wallichianafrom Uttarakhand region of India.International Journal of Green Pharmacy, 9(4): 246- 250.
Sheikh JH, Iyo Tsujiyama MT, Ashabul I, Rajat SB. 2008. Hitoshi, A. Total Phenolic Content, Anti-oxidative, anti- Amylase, Anti- Glucosidase and Anti-Histamine release activities of Bangladeshi fruits.Food Science and Technology Research,14(3): 261-268.
Shobana S, Sreerama YN, Malleshi NG. 2009. Composition and enzyme inhibitory properties of fnger millet (Eleusine coracana L.) seed coat phenolics: mode of inhibition of α- glucosidase and pancreatic amylase. Food Chemistry, 115(4): 1268–1273.
Singh B, Chopra A,Ishar MPS, Sharma A, Raj T. 2010. Pharmacognostic and antifungal investigations of Elaeocarpus ganitrus (Rudrakasha). Indian Journal of Pharmaceutical Sciences 72(2): 261-265.
Singh DK, Srivastva B,Sahu A. 2004. Spectrophotometric determination of Rauwolfia alkaloids, estimation of reserpine in pharmaceutical. Analytical sciences, 20(3): 571-573.
Singh TD, Meitei HT, Sharma AL, Robinson A, Singh LS, Singh TR. 2015. Anticancer properties and enhancement of therapeutic potential of cisplatin by leaf extract of Zanthoxylum armatum DC. Biological research,48(1): 1-9.
Swapana N, Jotinkumar T, Devi CB, Singh MS, Singh SB, Singh CB. 2012. Total Phenolic, Total Flavonoid Contents And Antioxidant Activity of A Fe w Indigenous Fruits Grown In Manipur. The bioscan, 7: 73-76.
Talukdar M, Duarah A, Talukdar S, Buragohain M, Debnath R, Yadav A, Jha DK , Bora TC, 2016. Bioprospecting Micromonospora from Kaziranga National Park of India and their anti-infective potential. World Journal of Microbiology and Biotechnology, 28(8): 2703–2712.
Tripathy S, Anil M, Sudhansu RS. 2016. Phytochemical screening and thin layer chromatographic studies of Elaeocarpus ganitrus Seed, the magical electromagnetic bead (Rudraksha). International Journal of Pharmacy and Biological Sciences, 6(3): P16-24.
Turner A, Bond DR, Vuong QV, Chalmers A, Beckett EL, Weidenhofer J, Scarlett CJ. 2020. Elaeocarpus reticulatus fruit extracts reduce viability and induce apoptosis in pancreatic cancer cells in vitro. Molecular biology reports, 47(3): 2073-2084.
Utami R, Nurhasniza K, MohdAspollah S, Mawardi R, Ahmad Bustaman A,Dachriyanus H. 2013. Phenolic contents, antioxidant and cytotoxic activities of Elaeocarpus floribundus Blume. Pakistan Journal of Pharmaceutical Sciences, 26(2): 245-250.
Vijayan A. 2017. Phytochemical Analysis of Elaeocarpus blascoi Weibel using Gas Chromatography – Mass Spectroscopy. Journal of Natural Products and Resources, 3(2): 125–129.
Vuong QV, Pham HNT, Vu HT, Dang TT, Van Ngo T, Chalmers AC. 2018. Fruit characteristics, phytochemical and antioxidant properties of blueberry ash (Elaeocarpus reticulatus). Heliyon, 4(10): e00834.
Worthington V. 1993. Alpha amylase, in: Worthington (Ed.), Worthington Enzyme Manual Freehold’, Worthington Biochemical Corporation. NJ, pp.36-41.
Yu L. 2001. Free radical scavenging properties of conjugated linoleic acids. Journal of Agricultural and Food Chemistry, 49(7): 3452-3456.
Zhishen J, Mengcheng T, Jianming W. 1999. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemisry, 64(4): 555-559. Zmarzty S. 2001. ‘Elaeocarpus in Southern India and Sri Lanka’, Kew Bulletin,56: 405-447.