Emre SEVİNDİK, MEHMET YAVUZ PAKSOY, Feyzanur TOPSEÇER, Melike AYDOĞAN

Phylogenetic Analysis of Chrysochamela (Fenzl) Boiss. (Brassicaceae) Taxa Growing in Turkey

In this study, we performed a genetic diversity analysis using RAPD (Random Amplified Polymorphic DNA) and ISSR (Inter simple sequence repeat) markers for some Chrysochamela species growing in Turkey. PCR was performed using RAPD and ISSR primers and genomic DNA samples from each specimen, and the resulting bands were scored. Phylogenetic relationships and genetic distances between the studied taxa were calculated using the PAUP analysis program. According to the RAPD analysis, the closest genetic distance was found to be 0.20000 between C. noeana populations, while the most distant values were found as 0.60000 between C. noeana and C. elliptica. The ISSR analysis also revealed the closest genetic distance as 0.34043 between C. noeana populations, while the most distant values were found to be 0.55319 between C. noeana and C. elliptica. UPGMA (unweighted pair group method with arithmetic mean) dendrogram generated for both RAPD and ISSR analyses consisted of two groups. As a result, it was found that RAPD data were more appropriate for comparison with the previous anatomical and morphological data of the studied taxa.

PDF

___

Ahmed NU, Park JI, Kim HR, Nou IS. 2012. Progress in Genetic Manipulation of the Brassicaceae. Journal of Plant Biotechnology, 39(1): 1-12.

Al-Shehbaz IA 2012. A generic and tribal synopsis of the Brassicaceae (Cruciferae). Taxon, 61: 931–954.

Al-Shehbaz IA, Beilstein MA, Kellogg EA. 2006. Systematics and phylogeny of the Brassicaceae (Cruciferae): an overview. Plant Syst. Evol, 259: 89-120.

Appel O, Al-Shehbaz IA. 2003. Cruciferae. In: Kubitzki, K. (Ed.). The Families and genera of Vascular Plants, Springer, Berlin Heidelberg 75-174.

Çakilcioglu U, Paksoy MY, Babacan EY, Polat R. 2017. Comparative Micromorphology and Anatomy of Chrysochamela (Fenzl) Boiss.(Cruciferae) Growıng in Turkey. Bang J. Bot, 46(2): 549-558.

Filiz E, Osma E, Kandemir A, Tombuloğlu H, Tombuloglu G, Birbilener S, Aydin M. 2014. Assessment of genetic diversity and phylogenetic relationships of endangered endemic plant Barbarea integrifolia DC.(Brassicaceae) in Turkey. Turk. J. Bot, 38(6): 1169-1181.

Ghasemi AR, Golparvar AR, Isfahani MN. 2014. Analysis of geneic diversity of sugar beet genotypes using random amplified polymorphic DNA marker. Genetika, 46(3): 975- 984.

Kavalcioğlu N, Açik L, Pınar NM. 2010. Comparative RAPD analysis and pollen structure studies of Bellis perennis L. Turk. J.Bot, 34(6): 479-484

Khorshidi S, Davarynejad G, Samiei L, Moghaddam M. 2017. Study of genetic diversity of pear genotypes and cultivars (Pyrus communis L.) using inter-simple sequence repeat markers (ISSR). Erwerbs-Obstbau, 59(4): 301-308.

Liu L, Zhao B, Tan D, Wang J. 2012. Phylogenetic relationships of Brassicaceae species based on matK sequences. Pak. J.Bot, 44(2): 619-626.

Raza A, Mehmood SS, Ashraf F, Khan RSA. 2019. Genetic diversity analysis of Brassica species using PCR-based SSR markers. Gesunde Pflanzen, 71(1): 1-7.

Sezer O, Öztürk D, Ocak A, Koyuncu O. 2017 Flora of Phrygian Valley (Mountain Phrygia/Turkey). Biodicon, 10(3): 163- 183.

Swofford DL. 2001. PAUP. Phylogenetic Analysis Using Parsimony (and other methods), Version 4.0b10.

Wang C, Cao J, Tian S, Wang Y, Chen Z, Chen M, Gong G. 2008. Germplasm resources research of Toona sinensis with RAPD and isoenzyme analysis. Biologia, 63(3): 320-326.

Yorgancılar M, Yakışır E, Erkoyuncu MT. 2015. Moleküler Markörlerin Bitki Islahında Kullanımı. Bahri Dağdaş Bitkisel Araştırma Dergisi, 4(2): 1-12.

Yüzbaşıoğlu S, Koch MA, Al-Shehbaz II. 2015. Proof of a knowledge database concept. Aubrieta ekimii (Brassicaceae), a new species from NW Anatolia (Turkey): morphological and molecular support. Plant Syst. Evol, 301(8): 2043-2055.