Optimization of Plant Regeneration in Different Pepper (Capsicum annuum L.) Lines
Development of an efficient plant regeneration protocol is essential for vegetable crop advancement by biotechnological methods. In this study, regeneration protocols of four pepper lines of different pepper types were optimized. Different protocols for organogenesis were investigated in regeneration experiments. Optimum plant regeneration was obtained in different combinations of 6- Benzylaminopurine (BAP) and Indole-3-acetic acid (IAA) in organogenesis assays. In organogenesis experiment, the highest shoot development was determined as 80% from hypocotyl explant of Demre pepper in 4 mg L-1 BA+0.5 mg L-1 IAA, 80% from hypocotyl explant of Charleston pepper in 3 mg L-1 BA+0.5 mg L-1 IAA, 80% from hypocotyl explant of capia pepper in 5 mg L-1 BA+0.5 mg L-1 and 84% from hypocotyl explant of bell pepper in 5 mg L-1 BA+0.5 mg L-1 IAA. Afterward, shoots were rooted and whole plants were obtained.
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Aboshama HMS. 2011. Direct somatic embryogenesis of pepper (Capsicum annuum L.). World Journal of Agricultural Sciences, 7(6):755-762.Agrawal S, Chandra N. 1983. Differentiation of multiple shoot buds and plantlets in culture embryos of Capsicum annuum L. var. Mathania. Curr. Sci., 52: 645-46.
Agrawal S, Chandra N, Kothari SL. 1989. Plant regeneration in tissue cultures of pepper (Capsicum annuum L. cv.Mathania). Plant Cell Tiss. Org., 16: 47-55.
Arous S, Boussaid M, Marrakchi M. 2001. Plant regeneration from zygotic embryo hypocotyls of Tunisian chili (Capsicum annuum L.). Journal of Applied Horticulture, 3: 17-22.
Akyavuz R, Taskin B, Koçak M, Yildiz M. 2018. Exploring the genetic variations and population structure of Turkish pepper (Capsicum annuum L.) genotypes based on peroxidase gene markers. 3 Biotech, 8(8): 355. doi: 10.1007/s13205-018-1380-2
Arroyo R, Revilla MA. 1991. In vitro plant regeneration from cotyledon and hypocotyl segments in two bell pepper cultivars. Plant Cell Rep., 10: 414-416.
Ashwani S, Ravishankar GA, Giridhar P. 2017. Silver nitrate and 2-(N-morpholine) ethane sulphonic acid in culture medium promotes rapid shoot regeneration from the proximal zone of the leaf of Capsicum frutescens Mill. Plant Cell Tiss. Org.,129(1): 175-180.
Bhutia KL, Meetei NT, Khanna VK. 2018. In vitro direct regeneration of Dalle Khursani (Capsicum annum) from salicylic acid treated explants. Journal of Pharmacognosy and Phytochemistry, 7(2): 1008-1012.
Christopher T, Rajam MV. 1994. In vitro clonal propagation of Capsicum spp. Plant Cell Tiss. Org., 38:25-9.
Christopher T, Rajam MV. 1996. Effect of genotype, explant and medium on in vitro regeneration of red pepper. Plant Cell Tiss. Org., 46: 245-50.
Dabauza M, Pena L. 2001. High efficiency organogenesis in sweet pepper (Capsicum annuum L.) tissues from different seedlings explants. Plant Growth Regul., 33: 221-9.
Ebida AIA, Hu CY. 1993. In vitro morphogenetic responses and plant regeneration from pepper (Capsicum annuum L. cv. Early California Wonder) seedling explant. Plant Cell Rep.,13:107-10.
El Nagar MM. 2012. Somatic embryogenesis of pepper (Capsicum annuum L.) and regeneration of transgenic plants after Agrobacterium-Mediated transformation. Journal of Applied Sciences Research, 8(11): 5550-5563.
Ezura H, Nishimiya S, Kasumi M. 1993. Efficient regeneration of plants independent of exogenous growth regulators in bell pepper (Capsicum annuum L.). Plant Cell Rep., 12: 676-80.
Franck-Duchenne M, Wang Y, Tahar SB, Beachy RN. 1998. In vitro stem elongation of sweet pepper in media containing 24- epi-brassinolide. Plant Cell Tiss. Org., 53(2): 79-84.
Gammoudi N, San Pedro T, Ferchichi A, Gisbert C. 2017. Improvement of regeneration in pepper: a recalcitrant species. In Vitro Cell Dev-Pl., 54(2):145-153.
Gunay AL, Rao P. 1978. In vitro plant regeneration from hypocotyl and cotyledon explants of red pepper (Capsicum). Plant Sci. Lett., 11: 365-72.
Haque SKM, Ghosh B. 2018. An improved micropropagation protocol for the recalcitrant plant Capsicum – a study with ten cultivars of Capsicum spp. (C. annuum, C. chinense, and C.frutescens) collected from diverse geographical regions of India and Mexico. The Journal of Horticultural Science and Biotechnology, 93: 1:91-99.
Hegde V, Partap PS, Yadav RC. 2017a. In vitro regeneration of Capsicum (Capsicum annuum L.) from cotyledon explants. International Journal of Current Microbiology and Applied Sciences, 6(5): 225-237.
Hegde V, Partap PS, Yadav RC. 2017b. Plant regeneration from hypocotyl explants in capsicum (Capsicum annuum L.). International Journal of Current Microbiology and Applied Sciences, 6(7): 545-557.
Husain S, Jain A, Kothari SL. 1999. Phenylacetic acid improves bud elongation and in vitro plant regeneration efficiency in Capsicum annuum L. Plant Cell Rep., 19: 64-8.
Hyde C, Phillips GC. 1996. Silver nitrate promotes shoot development and plant regeneration on chile pepper (Capsicum annuum L.) via organogenesis. In Vitro Cell DevPl.,32: 72-80.
Joshi A, Kothari SL. 2007. Copper levels in the medium improves differentiation and elongation from cultured cotyledons of Capsicum annuum L. Plant Cell Tiss. Org., 88: 127-33.
Kothari SL, Joshi A, Kachhwaha S, Ochoa-Alejo N. 2010. Chilli peppers - a review on tissue culture and transgenesis. Biotechnol. Adv., 28(1): 35-48.
Liu W, Parrott WA, Hildebrand DF, Collins GB, Williams EG. 1990. Agrobacterium induced gall formation in bell pepper (Capsicum annuum L.) and formation of shoot-like structures expressing introduced genes. Plant Cell Rep., 9: 360-4.
Madhuri V, Rajam MV. 1993. Apical shoot meristemculture in red pepper (Capsicum annuum L.). J. Plant Biochem. Biot., 2:67-8.
Maligeppagol M, Manjula R, Navale PM, Babu KP, Kumbar BM, Laxman RH. 2016. Genetic transformation of chilli (Capsicum annuum L.) with Dreb1A transcription factor known to impart drought tolerance. Indian J. Biotechnol., 15(1): 17-24.
Murashige T, Skoog F. 1962. A resived medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plantarum, 84(15): 473-497.
Ochoa-Alejo N, Ramírez-Malagón R. 2001. In vitro chili pepper biotechnology. In Vitro Cell Dev-Pl., 37(6): 701-729.
Orlinska M, Nowaczyk P. 2015. In vitro plant regeneration of 4 Capsicum spp. genotypes using different explant types. Turk. J. Biol., 39(1): 60-68.
Peddaboina V, Thamidala C, Karampuri S. 2003. Thidiazuron induced high frequency adventitious shoot formation and plant regeneration in Capsicum annuum L. Journal of Plant Biotechnology, 5(4):245-250.
Phillips GC, Hubstenberger JF. 1985. Organogenesis in pepper tissue cultures. Plant Cell Tiss. Org., 4: 261-9.
Ramírez-Malagón R, Ochoa-Alejo N. 1996. An improved and reliable chilli pepper (Capsicum annuum L.) plant regeneration method. Plant Cell Rep., 16: 226-31.
Renfiyeni S, Syukriani L, Asben A, Jamsari J. 2017. Optimal media for in-vitro regeneration of two local genotypes of chili pepper (Capsicum annuum L.) from West Sumatera. International Journal on Advanced Science, Engineering and Information Technology, 7(3): 904-909.
Sanatombi K, Sharma GJ. 2006. In vitro regeneration and mass multiplication of Capsicum annuum L. Journal of Food, Agriculture and Environment, 4: 205-8.
Sanatombi K, Sharma GJ. 2007a. Micropropagation of Capsicum frustescens L. using axillary shoot explants. Sci. Hortic., 113(1): 96-99.
Sanatombi K, Sharma GJ. 2007b. Micropropagation of Capsicum annum L. Not. Bot. Horti Agrobo., 35(1): 255-965.
Sanatombi K, Sharma GJ. 2008. In vitro plant regeneration in six cultivars of Capsicum spp. using different explants. Biol. Plantarum, 52(1):141-145.
Steinitz B, Wolf D, Matzevitch-Josef T, Zelcer A. 1999. Regeneration in vitro and genetic transformation of pepper (Capsicum spp.): The current state of art. Capsicum and Eggplant Newsletter, 18: 9-15.
Valadez-Bustos MG, Aguado-Santacruz GA, Carrillo-Castañeda G, Aguilar-Rincón VH, Espitia-Rangel E, Montes-Hernández S, Robledo-Paz A. 2009. In vitro propagation and agronomic performance of regenerated chili pepper (Capsicum spp.) plants from commercially important genotypes. In Vitro Cell Dev-Pl., 45:650. doi:10.1007/s11627-009-9193-y
- ISSN: 2148-127X
- Yayın Aralığı: Aylık
- Başlangıç: 2013
- Yayıncı: Turkish Science and Technology Publishing (TURSTEP)
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