Microbiological Quality of Raw Meat Sold in Tokat Province#

According to World Health Organization (WHO), an estimated 600 million people fall ill after eating contaminated food and 420 000 die every year. Although various foods can serve as sources of foodborne illness, meat has been at the forefront of societal concerns in recent years. Meat is source of some foodborne pathogens which have an important role on human health. In this study, it was aimed to examine the microbiological quality of raw bovine meat samples sold in Tokat province and to evaluate the results in the context of food safety. For this purpose, 18 raw meat samples purchased from butchers and markets were analyzed for total mesophilic aerobic bacteria, total psychrophilic aerobic bacteria, yeasts-molds, lactic acid bacteria, B. cereus, S. aureus,C. perfringens, total coliform, and fecal coliform. The presence of E. coli, E. coliO157:H7, L. monocytogenesand Salmonellaspp. were also investigated in these samples. The lowest and highest values determined for the parameters were as follows: total mesophilic aerobic bacteria 1.46×104-1.26×107CFU/g, total psychrotrofic aerobic bacteria 1.01×104-2.65×106CFU/g, yeasts-molds 3.00×103-1.70×104CFU/g, lactic acid bacteria 2.70×103-3.60×104CFU/g, B. cereus˂102-7.20×104CFU/g, S. aureus2.60×103-2.57×105CFU/g, C. perfringens˂102-9.20×103CFU/g, total coliform 3.80×101-2.90×104MPN/g, and fecal coliform

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Al-Mutairi MF. 2011. The incidence of Enterobacteriaceae causing food poisoning in some meat products.Advance Journal of Food Science and Technology.,3: 116-121.

Casaburi A. Piombino P. Nychas GJ. Villani F. Ercolini D. 2015. Bacterial populations and the volatilome associated to meat spoilage.Food microbiology.,45: 83-102.

CDC 2018. https://www.cdc.gov/fdoss/annual-reports/2017-report-highlights.html https://www.who.int/health-topics/food-safety/(E.T: 3.11.2019)

Çıtak S. Gündoğan N. Kala E. 2009. Ankara İlindeki Dondurulmuş Et Ve Sebzelerde Kolıform Ve Enterokokların Fekal İndikatör Bakteri Olarak Değerlendirilmesi.Türk Hijyen ve Deneysel Biyoloji Dergisi.,66: 145-151.

EFSA 2018 https://efsa.onlinelibrary.wiley.com/doi/full/10.2903/j.efsa.2018.5500 https://www.who.int/health-topics/food-safety/(E.T: 3.11.2019)FAOSTAT 2019. http://www.fao.org/faostat/en/#country/223(E.T: 3.11.2019)

FDA-BAM online, 2001a Aerobic Plate Count. In “FDA’s Bacteriological Analytical Manual, Edition 8, Chapter 3, https://www.fda.gov/food/laboratory-methods-food/bam-aerobic-plate-count(E.T: 21.10.2019)

FDA-BAM online, 2001b. Yeasts, Molds and Mycotoxins. In “FDA’s Bacteriological Analytical Manual, Edition 8, Chapter 18, https://www.fda.gov/food/laboratory-methods-food/bam-yeasts-molds-and-mycotoxins(E.T: 21.10.2019)

FDA-BAM online, 2001c. Clostridium perfringens.In “FDA’s Bacteriological Analytical Manual, Edition 8, Chapter 16. https://www.fda.gov/food/laboratory-methods-food/bam-clostridium-perfringens(E.T: 23.10.2019)

FDA-BAM online, 2001d. Staphylococcus aureus. In “FDA’s Bacteriological Analytical Manual” 8 th Edition, Revision A, Chapter 12. http://www.cfsan.fda.gov/~ebam/bam-toc.html. (E.T: 23.10.2019)

FDA-BAM online, 2002. Enumeration of Escherichia coliand the Coliform Bacteria. In “FDA’s Bacteriological Analytical Manual, Edition 8, Chapter 4, Revision 2013. https://www.fda.gov/food/laboratory-methods-food/bam-4-enumeration-escherichia-coli-and-coliform-bacteria(E.T: 23.10.2019)

FDA-BAM online, 2007.Salmonella. In “FDA’s Bacteriological Analytical Manual, Edition 8, Chapter 5, Revision 2018. https://www.fda.gov/food/laboratory-methods-food/bacteriological-analytical-manual-bam-chapter-5-salmonella(E.T: 30.10.2019)

FDA-BAM online, 2012. Bacillus cereus. In “FDA’s Bacteriological Analytical Manual,Edition 8, Chapter 14, Revision 2012. https://www.fda.gov/food/laboratory-methods-food/bam-bacillus-cereus (E.T: 23.10.2019)

Günşen U. Büyükyörük İ. 2005. Bazı Dondurulmuş Gıdalarda Mikrobiyolojik Kalite. Gıda Ve Yem Bilimi Teknolojisi Dergisi., 7:37-44.

ISO 11290, 1996. International Organization for Standardization, Microbiology of food and animal feding stuffs -Horizontal method for the detection and enumeration of Listeria monocytogenes, ISO 11290, İşviçre.

ISO 15214:1998, 1998. International Organization for Standardization, Microbiology of food and animal feding stuffs -Horizontal method for the enumeration of mesophilic lactic acid bacteria-colony count technique at 30°C,

ISO 15214:1998, İşviçre.ISO 16654:2001, 2010. International Organization for Standardization, Microbiology of food and animal feding stuffs -Horizontal method for the detection of Escherichia coliO157, ISO 16654:2001, İşviçre.

ISO 17410:2001, 2001. International Organization for Standardization, Microbiology of food and animal feding stuffs -Horizontal method for the enumeration of psychrotrophicmicro-organisms,ISO17410:2001, İşviçre.

Jablonski LM. Bohach GA.1997. Staphylococcus aureus. In: Doyle MP. Beuchat LR. Montville TJ. Food Microbiology Fundamentals and Frontiers. Washington D.C. ASM Pres. pp. 353-375. ISBN. 978-90-76998-06-0

Jay JM. 2000.Modern food microbiology. Gaithersburg, Md: Aspen Publishers. ISBN 0-8342-1671-X

Martin A. Beutin L. 2011. Characteristics Of Shiga Toxin-Producing Escherichia Coli From Meat And Milk Products Of Different Origins And Association With Food Producing Animals As Main Contamination Sources. International journal of food microbiology., 146: 99-104. DOI: 10.1016/j.ijfoodmicro.2011.01.041

Mboto CI. Agbo BE. Ikpoh IS. Agbor RB. Udoh DI. Ambo EE. Ekim MA. 2012. Bacteriological Study of Raw Meat of Calabar AbattoirWith Public Health and Veterinary İmportance. J. Microbiol. Biotech. Res., 2: 529-532.

Nursoy G. Akgün S. 1997. Ankara’daki Askeri Birliklerin İhtiyacı İçin Alınan Sığır Etlerinin Mikrobiyolojik Kaliteleri Üzerinde Araştırmalar. GIDA., 22: 241-245.

Omurtag AC, Türker S. 1978. Et ve Balık Kurumu Ankara Kombinasında Kesilip Piyasaya Arzedilen Parça-Paket Etlerin Hijyenik Kalitelerinin Mikrobiyolojik Yönden Analizleri Üzerinde Araştırma. Ankara Ecz. Far. Mec., 8: 90-100.

Ozbey A, Öncül N, Erdoğan K, Yıldırım Z, Yıldırım M. 2013. Tokat Yöresinde Üretilen Çalma Pekmezin Bazı Fiziksel, Kimyasal ye Mikrobiyolojik Özellikieri. Academic Food Journal/Akademik GIDA., 11:46-52.

Öztürk U. Gürbüz Ü. Çalım HD. 2006. Et Ve Et Ürünlerinde Mikrobiyolojik Kriterler Ve Halk Sağlığı Açısından Önemi. Türkiye 9. Gıda Kongresi. Bolu, 24-26 Mayıs 2006. pp. 24-26.

Pesavento G. Ducci B. Nieri D. Comodo N. Nostro AL. 2010. Prevalence And Antibiotic Susceptibility Of Listeria Spp. İsolated From Raw Meat And Retail Foods. Food Control., 21: 708-713. DOİ:10.1016/j.foodcont.2009.10.012

Stellato G. La Storia A. De Filippis F. Borriello G. Villani F. Ercolini D. 2016. Overlap Of Spoilage-Associated Microbiota Between Meat And The Meat Processing Environment İn Small-Scale And Large-Scale Retail Distributions. Appl. Environ. Microbiol., 82: 4045-4054. DOİ:10.1128/AEM.00793-16.

TGK 2011. Türk Gıda Kodeksi Mikrobiyolojik Kriterler Yönetmeliği. Sayı: 28157, Tarih: 29.12.2011WHO 2019. https://www.who.int/health-topics/food-safety/(E.T: 3.11.2019)

Yıldırım Z, Ceylan Ş. Öncül N. 2015. Tokat Piyasasında Satışa Sunulan Tavuk Etlerinin Mikrobiyolojik Kalitesinin Belirlenmesi. Akademik Gıda., 13: 304-316.

Yılmaz İ, Gümüş T. 2008. Sığır Karkaslarının Mikrobiyolojik Kalitesinin Belirlenmesi. Türkiye 10. Gıda Kongresi. Erzurum, 21-23 Mayıs 2008. pp. 525-528.

Yılmaz İ, Yılmaz E. 2012. Türkiye’de Hayvansal Gıda Tüketimi ve Sorunlar. Tarım, Yoksulluk ve Kalkınma., 10. Ulusal Tarım Ekonomisi Kongresi. Konya, 5-7 Eylül 2012. pp:981-984.