Nkechinyere Nweze, Reginald Njokuocha, Chiori Agwu, Nchedochukwu Ikegbunam, Okwong Walter

Melissopalynological Characterization of Honey Samples from Southeastern, Nigeria

The identification of plants in which the bees forage is key in establishing bee farms and increasing honey production. In this study pollen analysis of honey samples from the southeastern part of Nigeria was carried out to ascertain their floral sources and ecological origin. The honey samples were acetolyzed and microscopically studied to determine the pollen types. A total of seventy-one pollen types belonging to forty-one families of plants were identified. The honey samples were dominated by pollen grains from the families of Arecaceae, Euphorbiaceae, Myrtaceae, Irvigiaceae, Fabaceae, Combretaceae/Melastomataceae, and phyllanthaceae. Some of the dominant pollen grain identified include Elaeis guineensis, Alchornea cordifolia, Hymenocardia acida, Ocimum gratissimum, Syzygium guineense, Nauclea latifolia and Afzelia africana. Out of the six samples studied Njikoka sample was monofloral having Mimosa pigra as predominant pollen while Ayamelum, Ekwusigo, Nsukka, Ezeagu, and Udenu samples are multifloral containing Elaeis guineensis, Phyllanthus sp., Piliostigma reticulatum, Irvingia sp., Alchornea cordifolia, and Lannea sp. as major secondary pollen. All the samples analyzed have Elaeis guineensis in common except Ezeagu, indicating that these plants are present in all five locations. These results can also be used as a tool in the geographical identification of Southeastern Nigeria honey from other geopolitical zones

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Adekanmbi OH, Ogundipe OT. 2009. Nectar sources for the honey bee (Apis mellifera adansonii) revealed by pollen content. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 37(2): 211-217. http://dx.doi.org/10.15835/nbha3723245.
Adekanmbi OH, Walter OJ, Ikegbunam NC. 2019. Pollen analysis and heavy metals detection in honey samples from Southern Nigeria. World News of Natural Sciences, 26: 176-190.
Agwu COC, Obuekwe AI, Iwu MM. 1989. Pollen analytical and thin layer chromatographic examination of Nsukka (Nigeria) honey. Pollen et Spores, 31(1-2): 29-43.
Agwu COC, Abaeze CC. 1991. Palynological studies of four honeys from Anambra, Enugu and Kogi States of Nigeria. Journal of Agricultural Science and Technology, 1(2): 126-131.
Agwu COC, Akanbi TO. 1985. Palynological study of honey from four vegetation zones in Nigeria. Pollen et Spores, 27(3- 4): 335 - 348.
Agwu COC, Njokuocha RC. 2004. Pollen analysis of honey and the biological effects of honey as rooting medium. Nigerian Journal of Botany, 17: 74 - 82.
Aino DO. 2016. Pollen and physicochemical characterization of honey samples from Ankpa Local Government Area of Kogi State, Nigeria. Futo Journal Series, 2(2): 160 - 172.
Atanassova J, Bozilova E, Todorova S. 2004. Pollen analysis of honey from the region of three villages in West Bulgaria. Phytologia Balcanica, 10(2-3): 247 - 252.
Bonnefille R, Riollet G. 1980. Pollen Des Savanes D’Afrique Orientale. National Dela Recherche Scientifique, Paris. Bryant VMJ. 2001. Pollen content of honey. CAP Newsletter, 24(1): 10 - 24.
Emuobosa AO. 2017. Comparative pollen analysis of honeys from apiary and open markets in Nigeria and Bénin Republic. Ife Journal of Science, 19(2): 217-225
Erdtman G. 1969. Handbook of Palynology. An introduction to the study of Pollen grains and Spores. Hafnar Publishing Company, New York. 486 pp.
Fagundez GA, Caccavari MA. 2006. Pollen analysis of honeys from the central zone of the Argentine Province of Entre Rios. Grana, 45: 305 –320. doi: 10.1080/00173130601086636
Fallico B, Zappala M, Arena E, Verzera A. 2004. Effects of heating process on chemical composition and HMF levels in Sicilian monofloral honeys. Journal of Food Chemistry, 85: 305 - 313.
Fasasi KA. 2012. Physicochemical attributes of Nigerian honey from honey bees (Apis mellifera adansonii) (Hymenoptera: Apidae) and its shelf life in storage at room temperature. Pakistan Journal of Biological Science, 15(21): 1027 - 1033.
Forcone A. 2014. Pollen analysis of honey from Chubut (Argentinean, Patagonia). Grana, 47: 147 – 158. doi: 10.1080/10652460802106340.
Gosling WD, Miller CS, Livingstone DA. 2013. Atlas of the tropical West African pollen flora. Review of Palaeobotany and Palynology, 199: 1-135. doi: 10.1016/j.revpalbo.2013. 01.003.
Ige OE, Apo KA. 2007. Pollen analysis of honey samples from two vegetation zones in Nigeria. Science Focus, 13: 36 - 43.
Ige OE, Obasanmi OO. 2014. A Palynological assessment of honey samples from Delta State, Nigeria. American International Journal of Biology, 2(2): 47 - 59.doi: 10.1080/00173130410019497
Jones GD, Bryant Jr VM. 2004. The use of ETOH (Ethyl alcohol) for the dilution of honey. Grana, 43: 174 - 182.
Kayode J, Oyeyemi SD. 2014. Physicochemical investigation of honey samples from bee farmers in Ekiti State, Southwest Nigeria. Journal of Plant Science, 2(5): 246 - 249. doi: 10.11648/j.jps.20140205.26
Liberato C, Morais SM, Magalhães CEC, Magalhães IL, Cavalcanti DB, Silva MMO. 2013. Physicochemical properties, mineral and protein content of honey samples from Ceará State, Northeastern Brazil. Food Science Technology Campinas, 33(1): 38 - 46.
Louveaux J, Maurizio A, Vorwohl G. 1978. International Commission for Bee Botany of IUBS. Methods of Melissopalynology. Bee World, 59: 139–157.
Mbah CE, Amao AO. 2009. Natural foods and feeding habits of the African honey Bees Apis mellifera adansonii in Zaria, Northern Nigeria. Science World Journal, 4(1): 11 - 14.
Moar NT. 2014. Pollen analysis of New Zealand honey. New Zealand Journal of Agricultural Research 28(1): 39 – 70. doi: 10.1080/00288233.1985.10426997
Njokuocha RC, Ekweozor CC. 2007. Pollen contents of commercial honeys from Opi, Nsukka, and Enugu state, Nigeria. Plants Products Research Journal, 11: 5 - 11.
Njokuocha RC, Nnamani NA. 2009. Palynological analysis of seven samples of honey from the rainforest-savannah vegetation of east-middle belts of Nigeria. Nigerian Journal of Botany, 22(1): 189 - 202.
Njokuocha RC, Osayi EE. 2015. Physicochemical assessment and pollen analysis of honey from Nsukka, Nigeria. Nigerian Journal of Botany, 28(1): 95 - 107.
Njokuocha RC, Dim KI, Onyejekwe OK, Nwokorie VU. 2019. Determination of the concentration of some mineral elements and pollen spectra of Apis mellifera L. honeys from different locations in Nigeria, Animal Research International, 16(1): 3186-3197.
Nnamani VC, Uguru AN. 2013. Diversity Of honey producing plants of Southern Nigeria: Basic prerequisite for conservation. Journal of Sustainability and Science Management, 8(1): 103 - 112.
Okereke NC, Mbaekwe EI, Nnabude PC, Ekwealor KU, Nwonumara GU, Iroka CF, Ukpaka CG. 2016. Comparative evaluation of the species richness and diversity of three parallel forest ecosystems in Southeastern Nigeria. Journal of Agriculture and Ecology Research International, 8(4): 1 - 12.
Pfister R. 1895. Versuch einer Mikroskopie des Honigs. Foschungsber. – Lebensmitt. Bez. Hyg. Chemical Pharmaceutical (Mu ̈nchen), 2: 29.
Ramírez-Arriaga E, Navarro-Calvo LA, Díaz-Carbajal E. 2011. Botanical characterisation of Mexican honeys from a subtropical region (Oaxaca) based on pollen analysis. Grana, 50(1): 40 - 54.
Ruoff K, Bogdanov S. 2004. Authenticity of honey and other beeproducts. Apiacta, 38: 317 - 327.
Sadia B, Husan SZ, Malik RN. 2008. Pollen analysis and heavy metal detection in honey samples from seven selected countries. Pakistan Journal of Botany, 40(2): 507 - 516.
Shah A, Sikandar F, Ullah I, Khan SU, Rana UA, McCoy T. 2014. Spectrophotometric determination of trace elements in various honey samples collected from different environments. Journal of Food and Nutrition Research, 2(9): 532 - 538.
Y’bert JP. 1979. Atlas Des pollen De cote D’ Ivoire. O.R.S.T.M, Paris.
Yédomonhan H. 2009. Plantes mellifères et potentialités de production de miel en zones guinéenne et soudano-guinéenne au Bénin. Thèse de doctorat, université d'Abomey-Calavi, 366p