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Diferansiyel PCR, Konvansiyonel PCR ve Nested PCR Testleri ile Canine Parvovirus'un Karşılaştırmalı Tespiti: Diferansiyel PCR ile Antijenik Varyantların Tespiti

Canine parvovirus (CPV), yavru köpeklerde hemorajik enterite neden olan ve esas olarak feko-oral yolla bulaşan bir patojendir. Bu çalışmada, CPV’den şüphelenilen toplam 35 hayvandan dışkı örneği toplandı. Örnekler konvansiyonel PCR, Nested-PCR ve Diferansiyel PCR testi ile incelendi. 35 köpekten 20 tanesi (%57,1) konvansiyonel PCR ile 31 tanesi Nested-PCR (%88,6) ile ve 30 tanesi de (%85,7) diferansiyel PCR ile pozitif olarak belirlendi. CPV-2a’nın en yaygın antijenik tip olduğu, erkek hayvanların ve 0-3 aylıkların hastalığa yakalanma oranının yüksek olduğu ve nadir olarak da aşılı hayvanların da hastalığa yakalanabileceği belirlendi. Bu doğrultuda mevcut subtipleri tespit etmek, güvenilir teşhis ve aşı yöntemleri geliştirmek amacıyla moleküler epidemiyoloji sürveyansı sağlayan çalışmalara ağırlık verilmesi tavsiye edilmektedir.

Comparative Detection of Canine Parvovirus by Differential PCR, Conventional PCR and Nested PCR tests: Detecting Antigenic Variants by Differential PCR

Canine parvovirus (CPV) is a pathogen causing hemorrhagic enteritis in puppies and mainly transmitting via feco-oral route. In this study, stool samples were collected from a total of 35 animals suspected of CPV. The samples were examined by Conventional PCR, Nested PCR and Differential PCR tests. 20 out of 35 dogs (57.1%) were detected positive by conventional PCR, 31 (88.6%) by nested PCR and 30 (85.7%) by differential PCR. CPV 2a was stated as the most common antigenic type, male animals and 0–3-month-olds had a high rate of becoming sick and vaccinated animals might also catch the disease, rarely. Accordingly, it is recommended to focus on studies providing molecular epidemiology surveillance in order to detect the existing subtypes and develop reliable diagnosis and vaccination methods.

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