Emre Hastaoğlu, Nazlı Ercan, Sema Ağaoğlu

Investigation of Beta-Lactam and Tetracycline Group Antibiotic Residues in Bovine Liver, Kidney and Muscle Tissue

In this study, beta-lactam and tetracycline antibiotic residues were investigated in cattle liver, kidney and muscle samples. For this purpose, a total of 75 bovine tissue samples (each of 25 from liver, kidney, muscle) taken from 25 cattle slaughtered in a local slaughterhouse in Sivas were used as materials. ELISA method was applied in the analysis and it was studied with commercial test kits. According to the results of the analysis; beta-lactam and tetracycline residues were detected in all bovine tissue samples. Beta-lactam level was determined between 0.75-1.07 ppb (mean 0.94 ± 0.01) in liver samples, 0.67-1.05 ppb (mean 0.81 ± 0.01) in kidney samples and 0.70-2.57 ppb (mean 0.97 ± 0.07) in muscle samples. Tetracycline level was detected in the range of 4.48-8.50 ppb (mean 6.14 ± 0.17) in liver samples, 1.73-6.39 ppb (mean 4.90 ± 0.24) in kidney samples and 3.31-7.45 ppb (mean 5.67 ± 0.25) in muscle samples. The residue levels determined in the examples complied with the legal limits reported in the European Commission and the Turkish Food Codex Communiqué.

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Abbasi MM, Rashidi MR, Javadi A, Bannazadeh M, Mirmahdavi S, Zabihi M. 2009. Levels of tetracycline residues in cattle meat, liver and kidney from a slaughter house in Tabriz, Iran. Turkish Journal of Veterinary and Animal Science, 33(4):345-349.
Abbasi MM, Nemati MH, Babaei H, Ansarin M, Nourdadgar A. 2012. Solid-Phase extraction and simultaneous determination of tetracycline residues in edible cattle tissues using and HPLC-FL method. Iranian Journal of Pharmaceutical Research, 11(3):781-787.
Abdullah OA, Shareef AM, Sheet OH. 2012. Detection of streptomycin residues in local meat of bovine and ovine. Iraqi Journal of Veterinary Science, 26(1):43-46.
Agmas B, Adugna M. 2018. Antimicrobial residue occurrence and its public health risk of beef meat in Debre Tabor and Bahir Dar, Northwest Ethiopia. Veterinary World, 11(7):902- 908.
Al-Gendy HM, Hasanen FS, Salem AM, Nada SM. 2014. Assessment of oxytetracycline and ampicillin residues in sheep carcasses. Benha Veterinary Medical Journal, 27(2):188-196
Baghani A, Mesdaghinia A, Rafieiyan M, Dallal MMS, Douraghi M. 2019. Tetracycline and ciprofloxacin multiresidues in beef and chicken meat samples using indirect competitive ELISA. Journal of Immunoassay and Immunochemistry, 40(3):328- 342.
Beyene T. 2016. Veterinary drug residues in food-animal products: Its risk factors and potential effects on public health. Journal of Veterinary Science and Technology, 7:1-7.
Bilashoboka E. 2016. Assessment of oxytetracycline residue levels in beef consumed in Dodoma municipality catchment area, Tanzania. Masters of Science in Epidemiology and Biostatistics. Department of Public Health, the University of Zambia.
Bilashoboka E, Mudenda B, Munyinda N, Moshi FV, Kambarage DM. 2019. Determination of oxytetracycline residue levels in edible tissues of slaughtered cattle. Food and Environment Safety Journal, 17(4).
Biswas AK, Rao GS, Kondaiah N, Anjaneyulu ASR, Mendiratta SK, Prasad R, Malik JK. 2007. Simple multi-residue method for determination of oxytetracycline, tetracycline and chlortetracycline in export buffalo meat by HPLC-photodiode array detector. Journal of Food and Drug Analysis, 15(3):278- 284.
Codex Alimentarius (CAC). 2018. Maximum Residue Limits (MRLs) and Risk Management Recommendations (RMRs) for Residues of Veterinary Drugs in Foods. International Food Standards, CX/MRL 2-2018.
Darwish WS, Eldaly EA, El-Abbasy MT, Ikenaka Y, Nakayama S, Ishizuka M. 2013. Antibiotic residues in food: The African scenario. Japanese Journal of Veterinary Research 61(Supplement), 513-522.
Elbagory AM, Edris AM, Muhammad KM. 2017. Studies and residues of antibiotics and growth enhancer-hormone in imported and locally produced beef. Nutrition and Food Technology: Open Access, 3(2).
Emiri A, Myftari E, Çoçoli S, Treska E. 2014. Determination of oxytetracycline, tetracycline and chlortetracycline in beef meat by HPLC-DAD detector in Albania. Albanian Journal of Agricultural Sciences (Special edition), 489-493.
Er B, Onurdağ FK, Demirhan B, Özgacar SÖ, Öktem AB, Abbasoğlu U. 2013. Screening of quinolone antibiotic residues in chicken meat and beef sold in the markets of Ankara, Turkey. Poultry Science, 92(8):2212-2215.
Erdoğdu AT, Koçyiğit Y, Özdemir G, Coşkun Y. 2009. Tüketime sunulan sığır ve koyun etlerinde tetrasiklin türevi antibiyotiklerin kalıntılarının belirlenmesi. 3. Ulusal Veteriner Gıda Hijyeni Kongresi Kitabı, p. 175, Bursa.
European Commission. 2010. Commission Regulation (EU) No 37/2010 of 22 December 2009 on Pharmacologically Active Substances and Their Classification Regarding Maximum Residue Limits in Foodstuffs of Animal Origin. Official Journal of the European Union, 15:1-72.
Jayalakshmi K, Paramasivam M, Sasikala M, Tamilam TV, Sumithra A. 2017. Review on antibiotic residues in animal products and its impact on environments and human health. Journal of Entomology and Zoology Studies, 5(3):1446-1451.
Kaya S. 2018. Hayvansal gıdalarda ilaç kalıntıları ve tüketici sağlığı. International Journal of Scientific and Technological Research, 4(8): 28-37.
Kaya S, Yavuz H, Akar F, Liman BC, Filazi A. 1992. Mezbahadan sağlanan sığır et, karaciğer ve böbrek örneklerinde antibiyotik kalıntıları. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 39(1-2):13-29.
Kimera ZI, Mdegela RH, Mhaiki CJN, Karimuribo ED, Mabiki F, Nonga HE, Mwesongo J. 2015. Determination of oxytetracycline residues in cattle meat marketed in the Kilosa district, Tanzania. Onderstepoort Journal of Veterinary Research, 82(1):911.
Koç F. 2006. Screening of quinolone residues in animal tissues. Indian Veterinary Journal, 83:1063-1064.
Landers TF, Cohen B, Wittum TE, Larson EL. 2012. A review of antibiotic use in food animals: perspective, policy, and potential. Public Health Reports, 127(1):4-22.
Mensah SEP, Koudande OD, Sanders P, Laurentie M, Mensah GA, Abiola FA. 2014. Antimicrobial residues in foods of animal origin in Africa: Public health risks. Scientific and technical review (International Office of Epizootics), 33(3):987-996, 975-986.
Mgonja F, Mosha R, Mabiki F, Choongo K. 2017. Oxytetracycline residue levels in beef in Dodoma region, Tanzania. African Journal of Food Science, 11(2), 40-43. Muriuki FK, Ogara WO, Njeruh FM, Mitema ES. 2001.
Tetracycline residue levels in cattle meat from Nairobi slaughterhouse in Kenya. Journal of Veterinary Science, 2(2):97-101.
Nchima G, Choongo K, Muzandu K, Nalubamba K, Muma J, Bumbangi F, Mongal G, Kangwa H. 2017. Determination of oxytetracycline and sulphamethazine residues in marketed beef from selected parts of Zambia to assess compliance with maximum residual limits. American Journal of Research Communication,5(9):42-69.
Nisha AR. 2008. Antibiotic residues-a global health hazard. Veterinary World, 1(12):375-377.
Olatoye IO, Ehinmowo AA. 2010. Oxytetracycline residues in edible tissues of cattle slaughtered in Akure, Nigeria. Nigerian Veterinary Journal, 31(2):93-102.
Oruç HH, Cengiz M, Bağdaş D, Uzunoğlu İ. 2007. Sığır etlerinde streptomisin ve sulfametazin (sulfadimidin) kalıntıları. Uludağ University Journal of the Facult of Veterinary Medicine, 26 (1-2):17-20.
Ramatla T, Ngoma L, Adetunji M, Mwanza M. 2017. Evaluation of antibiotic residues in raw meat using different analytical methods. Antibiotics, 6(4):34.
Shea KM. 2003. Antibiotic resistance: what is the impact of agricultural uses of antibiotics children’s health? Pediatrics, 112(1-2):253-258.
Tadesse T, Tadesse T. 2017. Public health impacts of antibiotic residues in foods of animal origin: A review. Public Policy and Administration Research, 7(10):6-11.
Tayar M, Yarsan E. 2014. Veteriner Halk Sağlığı. 1. Baskı. Dora Yayın, Bursa. Tayar M, Yıldırım Y. 2020. Et Endüstrisi. 1. Baskı. Dora Yayın, Bursa. Tesfaye S. 2019. A review on the antimicrobial residues and their health impacts in Ethiopia. International Journal of Microbiological Research, 10(3):139-147.
Türk Gıda Kodeksi (TGK). 2017. Hayvansal gıdalarda bulunabilecek farmakolojik aktif maddelerin sınıflandırılması ve maksimum kalıntı limitleri yönetmeliği. Resmi Gazete, 7 Mart 2017, Sayı: 30000, Başbakanlık Basımevi, Ankara.
Türkiye Bilimler Akademisi (TÜBA). 2017. İnsan ve hayvan sağlığında akılcı antibiyotik kullanımı ve antibiyotik dirençlilik raporu. Ed. Prof. Dr. Kazım Şahin. Türkiye Bilimler Akademisi Yayınları, Tüba Raporları, No:21, Ankara.
Vougat Ngom RRB, Garabed RB, Rumbeiha WK, Foyet HS, Schrunk DE, Shao D, Pagnah AZ. 2017. Penicillin-G and oxytetracycline residues in beef sold for human consumption in Maroua, Cameroon. International Journal of Food Contamination, 4:17.
Vragovic N, Bazulic D, Nijari B. 2011. Risk assessment of streptomycin and tetracycline residues in meat and milk on Croatian market. Food Chemical Toxicol, 49:352-355.
Yüksek N. 2001. Etlerde antibiyotik kalıntılarının aranması üzerinde çalışmalar. Doktora tezi. Uludağ University Journal of the Faculty of Veterinary Medicine, 20:85-90.