Sevda Kirbağ, Şule İnci, Ayşe Eren

Determination of Antimicrobial and Antioxidant Activity of Alchemilla alpina L.

Alchemilla genus, which belongs to the Rosaceae family, is a medicinal plant used for various purposes among the people. Species of this genus are known in Turkish folk medicine as lion claw or hazelnut grass. Especially, they are used mainly women’s illnesses, in gastritis, anti- inflammatory, as carminative, and in the treatment of wound. Besides the antimutagenic effect of Alchemilla alpina L., its above-ground parts are used for antimycotic purposes in the form of tea or oral care water. In this study, it has been aimed to determine the antimicrobial effect of the above- ground parts of Alchemilla alpina extracts obtained from methanol, ethanol and chloroform and the antioxidant activity of different concentrations of the extract obtained from methanol. The antimicrobial activity of methanol, ethanol and chloroform extracts of the above-ground parts of A. alpina has been determined according to disk disc diffusion method. In the results obtained have been showed that these extracts inhibited the growth of some bacteria (Staphylococcus aureus ATCC25923, Escherichia coli ATCC25322, Klebsiella pneumoniae ATCC700603, Bacillus megaterium DSM32) and yeasts (Candida albicans FMC17 and Candida glabrata ATCC66032) at different rates (8-23 mm). The antioxidant activity of different concentrations (1.25, 2.5, 5 and 10 mg/ml) of the above-ground parts of A. alpina extract obtained from methanol has been determined according to the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity method. In the results obtained, it has been observed that the effect of removing DPPH radical of A. alpina increased depending on increasing concentrations.

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Acet T, Kadriye Özcan K. 2018. Determination Of antioxidant and antimicrobial properties of lady's mantle (Alchemilla ellenbergiana) extracts. Gümüşhane Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 8 (1): 113-121. doı: 10.17714/gumusfenbil.308430
Altundag E, Ozturk M. 2011. Ethnomedicinal studies on the plant resources of East Anatolia, Turkey, Procedia Social and Behavioral Sciences, 19: 756–777. doi: Http://Dx.Doi.Org/10.1016/J.Sbspro.2011.05.195
Banerjee J, Das A, Sinha M, Saha S. 2018. Biological efficacy of medicinal plant extracts in preventing oxidative damage. Oxidative Medicine and Cellular Longevity, 2018: 2. doi: https://doi.org/10.1155/2018/7904349
Çelik A, Ayran İ. 2020. Antioksidan kaynağı olarak bazı tıbbi ve aromatik bitkiler. Türk Bilimsel Derlemeler Dergisi, 13(2): 115-125.
Davis, P. H. (1972). Flora of Turkey and the Aegean Islands. V:4 Edinburgh Univ. Press. England.
Denev P, Kratchanova M, Ciz M, Lojek A, Vasicek O, Blazheva D, Nedelcheva P, Vojtek L, Hyrsl P. 2014. Antioxidant, antimicrobial and neutrophil-modulating activities of herb extracts. Acta Biochimica Plonica, 61, 359–367. doi: 10.18388/abp.2014_1907
Dimitrova DZ, Nedialkov P, Kitanov G. 2010. Radical scavenging and antioxidant activities of methanolic extracts from Hypericum species growing in Bulgaria. Pharmacognosy Magazine, 6, 74-78. doi: 10.4103/0973- 1296.62889.
Ege M, Elmastaş M. 2020. Antiviral etkili fitoterapötikler: tıbbi bitkiler ve fitokimyasallar. Journal Of Integrative And Anatolian Medicine, 1(2): 5-20.
Collins CM, Lyne PM. 2004. Microbiological methods. In: Collins CH, Lyne PM, Grange JM, Flkinham III JO, editors. Mycological methods. 8 ed. London, p. 140. ISBN 0-340- 80896-9
İlgün S, Baldemir A, Koşar M. 2014. Alchemilla L. türlerinin kimyasal bileșikleri ve biyolojik aktiviteleri. Hacettepe University Journal of the Faculty of Pharmacy, 34, 17-30.
Kaya E, Şeker Karatoprak G, Koşar M, Özbilge H, Başer KHC. 2009. Antimicrobial Properties Of Alchemilla mollis, Ankara, Türkiye, 23 June- 26 September 2009, 9th International Symposium On Pharmaceutical Sciences (ISOPS-9).
Kaya B, Artuvan Y. 2016. Investigation of antioxidant and antimicrobial effect, phenolic compounds of Alchemilla cimilensis. El-Cezerî Journal Of Science And Engineering, 3(1), 27-54.
Şeker Karatoprak G, İlgün S, Koşar M. 2017. Phenolic composition, anti-ınflammatory, antioxidant, and antimicrobial activities of Alchemilla mollis (Buser) Rothm. Chemistry & Biodiversity, 14 (9): e1700150. doi: https://doi.org/10.1002/cbdv.201700150
Mohammed FS, Pehlivan M, Sevindik M. 2019. Antioxidant, antibacterial and antifungal activities of different extracts of Silybum marianum collected from Duhok (Iraq). International Journal of Secondary Metabolite, 6(4): 317-322.
Mohammed FS, Şabik AE, Sevindik E, Pehlivan M, Sevindik, M. 2020. Determination of Antioxidant and Oxidant Potentials of Thymbra spicata Collected from Duhok-Iraq. Turkish Journal of Agriculture-Food Science and Technology, 8(5): 1171-1173. DOI: 10.24925/turjaf.v8i5.1171-1173.3341
Mohammed FS, Kına E, Sevindik M, Dogan M, Pehlivan M. 2021b. Datura stramonium (Solanaceae): Antioxidant And Antimicrobial Potentials. Turkish Journal Of Agriculture - Food Science And Technology, 9(4): 818-821. DOI: https://doi.org/10.24925/turjaf.v9i4.818-821.4264
Mohammed FS, Pehlivan M, Sevindik E, Akgul H, Sevindik M, Bozgeyik I, Yumrutas O. 2021a. Pharmacological properties of edible Asparagus acutifolius and Asparagus officinalis collected from North Iraq and Turkey (Hatay). Acta Alimentaria, 50(1): 136–143. doi: https://doi.org/10.1556/066.2020.00204
Murathan ZT. 2018. Kuzeydoğu anadolu bölgesi ekolojik koşullarında yetişen bazı tıbbi bitkilerin biyokimyasal içeriği ve antioksidan özelliklerinin belirlenmesi. Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 20(2): 51-60. doi: 10.25092/baunfbed.468493
Oktyabrsky O, Vysochina G, Muzyka N, Samoilova Z, Kukushkina T, G. Smirnova G. 2009. Assessment of anti- oxidant activity of plant extracts using microbial test systems. Journal Of Applied Microbiology, 106: 1175–1183. Doi: https://doi.org/10.1111/j.1365-2672.2008.04083.x
Onbaşlı D, Yuvalı Çelik G, Türk Katırcıoğlu H. 2020. İlaca dirençli mikroorganizmalara karşı yeni bir yaklaşım: geleceğin antibiyotikleri, bitkisel antimikrobiyal peptidler. Erciyes Üniversitesi Sağlık Bilimleri Fakültesi Dergisi, 7(1): 51-57.
Ozbek H, Acikara OB, Keskin I, Kirmizi NI, Ozbilgin S, Ergene Oz BE, Kurtul E, Ozrenk BC, Tekine M, Saltan G. 2017. Evaluation Of Hepatoprotective And Antidiabetic Activity Of Alchemilla mollis. Biomedicine & Pharmacotherapy, 86: 172–176. doi: https://Doi.Org/10.1016/J.Biopha.2016.12.005
Özbek O, Budak Y, Özyiğit Ç, Yanar Y. 2021. Bitki patojeni funguslara karşı Grindelia robusta Nutt., Tanacetum praeteritum Subsp. praeteritum ve Alchemilla vulgaris L. ekstraktlarının antifungal aktivitelerinin değerlendirilmesi. Turkish Journal of Science and Health, 2(1): 57-6.
Pehlivan M, Mohammed FS, Sevindik M, Akgul H. 2018. Antioxidant and oxidant potential of Rosa canina. Eurasian Journal of Forest Science, 6(4): 22-25.
Sharma OP, Bhat TK. 2009. DPPH antioxidant assay revisited. Food Chemistry, 113: 1202–1205. doi:10.1016/ J.Foodchem.2008.08.008Get
Şeker Karatoprak G, İlgün S, Koşar M. 2017. Phenolic composition, anti-ınflammatory, antioxidant, and antimicrobial activities of Alchemilla mollis (Buser) Rothm. Chemistry & Biodiversity, 14 (9): e1700150. doi: https://doi.org/10.1002/cbdv.201700150
Sevindik M, Akgul H, Pehlivan M, Selamoglu Z. 2017. Determination of therapeutic potential of Mentha longifolia ssp. longifolia. Fresen Environ Bull, 26(7): 4757-4763.
The Plant List 2021. Publish On The İnternet. Available from: http://www.theplantlist.org/tpl1.1/record/kew-2523483 [Accessed April 2021]
Uçar Sözmen E, Eruygur N, Akpulat Ha, Çetın Md, Durukan H, Demırbaş A, Karaköy T. 2020. Sivas ili doğal florasından toplanan sarı kantaron (Hypericum scabrum L.) ve aslan pençesi (Alchemilla mollis (Buser) Rothm.) bitkilerinin bazı kalite kriterlerinin belirlenmesi. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 10(2): 1410-1418. doi: 10.21597/jist.655128
Uritu CM, Mihai CT, Stanciu GD, Dodi G, Alexa-Stratulat T, Luca A, Leon-Constantin MM, Stefanescu R, Bild V, Melnic S, Tamba BI. 2018. Medicinal plants of the family Lamiaceae in pain therapy: a review. Pain Research and Management, 2018: 44. doi: https://doi.org/10.1155/2018/7801543
Usta C, Yildirim AB, Turker AU. 2014. Antibacterial and antitumour activities of some plants grown in Turkey. Biotechnology & Biotechnological Equipment, 28(2), 306- 315. doi: 10.1080/13102818.2014.909708
Viegi L, Pieroni A, Guarrera PM, Vangelisti R. 2003. A review of plants used in folk veterinary medicine in Italy as basis for a databank. Journal Of Ethnopharmacology, 89(2-3), 221- 244. doi: https://doi.org/10.1016/j.jep.2003.08.003