In vitro Multiple Shoot Regeneration from Petunia hybrida

An efficient plant regeneration protocol was developed from in vitro germinated seeds of Petunia hybrida an ornamentally important plant in the family Solanaceae. Shoot tip and node explants of Petunia hybrida were cultured on MS basal medium supplemented with different concentrations and combinations of Benzyl amino purine (BAP), 1-Naphthaleneacetic acid (NAA), Indole-3-butyric acid (IBA) and Gibberellic acid (GA3). The highest shoot length was obtained from MS medium supplemented with 1 mg/l BAP + 1 mg/l NAA. The highest shoot number (3 shoots/explant) were obtained from MS medium supplemented with 0.6 mg/l BAP + 0.5 mg/l IBA. The isolated shoots were transferred to MS basal medium supplemented with different concentrations of GA3 ranging from 0.05, 0.2, 0.5 and 1 mg/l for shoot elongation. The highest shoot length (5.75 cm) was recorded from the MS medium supplemented with 0.2 mg/l GA3 +0.2 mg/l BAP. Rooting of regenerated shoots were achieved on MS medium supplemented with 0.1-1 mg/1 IBA and NAA. The regenerated shoots with well developed roots were successfully acclimatized and established in pots containing sterilized peat moss and grown under laboratory conditions with 70% survival rates.

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Abu-Qaoud H, Abu-Rayya A, Yaish S. 2010. In vitro regeneration and somaclonal variation of Petunia hybrida. Journal of Fruit and Ornamental Plant Research, 18(1): 71- 81.

Annapurna D, Rathore TS. 2010. Direct adventitious shoot induction and plant regeneration of Embelia ribes Burm F. Plant Cell, Tissue and Organ Culture (PCTOC), 101(3): 269- 277.

Binding H. 1971. Organogenese and kallus von Petunia hybrida. Zeitschrift fur Pflanzenphysiologie, 359-364.

Dash SN, Singhsamant PK. 1990. Induction of plantlets and callus from shoot-tips of Petunia hybrida cultured in vitro. Orissa Journal of Horticulture, 18(1-2): 65-69.

Fosket DE. 1980. Hormonal control of morphogenesis in cultured tissues. In Plant Growth Substances 1979, Springer Berlin Heidelberg, 362-369.

Hailu T, Abera B, Daksa J. 2015. In Vitro direct organogenesis protocol for mass propagation of an elite Ethiopian hot pepper (Capsicum annuum L.) cultivar mareko fana. American Journal of Plant Sciences, 6(9): 1435.

Hartmann HT, Kester DF. 1983. Plant propagation principles and practices. 4 Ed. Prentice Hall, IWC. England, New Jersey, pp: 276.

Husain S, Jain A, Kothari SL. 1999. Phenylacetic acid improves bud elongation and in vitro plant regeneration efficiency in Capsicum annuum L. Plant Cell Reports, 19(1): 64-68.

Khadiga G, Rasheid SM, Mutasim M K. 2009. Effect of Cultivar and Growth Regulator on In vitro Micropropagation of Potato (Solanum tuberosum L). American-Eurasian Journal of Sustainable Agriculture, 3(3): 487-492.

Kharrazi IVM, Nemati H, Tehranifar A, Bagheri A, Sharifi A. 2011. Culture of Carnation (Dianthus caryophyllus L.) Focusing on the Problem of Vitrification. Journal of Biological and Environmental Sciences, 5(13): 9-26.

Kulpa D, Nowak N. 2011. In vitro flowering of Petunia× atkinsiana D. Don. Folia Horticulturae, 23(2): 125-129.

Laloue M. 1977. Cytokinins: 7-glucosylation is not a prerequisite of the expression of their biological activity. Planta, 134(3): 273-275.

Letham DS, Palni LMS. 1983. The biosynthesis and metabolism of cytokinins. Annual Review of Plant Physiology, 34(1): 163-197.

Li F, Li C, Li M, Yu M, Fang, C, Wang S. 2013. In vitro culture of Petunia hybrida microspores and Agrobacterium-mediated transient expression of β-glucuronidase (GUS) reporter gene. Int. J. Agric. Biol, 15: 1098-1104.

Mahadev MD, Panathula CS, Naidu CV. 2014. Efficient protocol for direct shoot organogenesis from in vitro raised nodal explants of Solanum viarum (Dunal)-An important anticancer medicinal plant. International Journal of Medicinal and Aromatic Plants, 4(1): 48-55.

Murashige T, Skoog F. 1962. Revised medium for rapid growth and bioassay with tobacco micro-propagated. Physiologia Plantarum, 15: 473-9.

Otroshy M, Moradi K, Khayam Nekouei M. 2011. The effect of different cytokenins in propagation of capsicum annuum L. by in vitro nodal cutting. Trakia Journal of Sciences, 9(3): 21- 30.

Quattrocchio F, Wing J, Van der Woude K, Souer E, De Vetten N, Mol J, Koes R. 1999. Molecular analysis of the anthocyanin 2 gene of petunia and its role in the evolution of flower color. The Plant Cell, 11(8): 1433-1444.

Rao PS, Handro W, Harada H. 1973. Bud formation and embryo differentiation in in vitro cultures of Petunia. Zeitschrift für Pflanzenphysiologie, 69(1): 87-90.

Sabitha C, Jawaharlal M, Ganga M, 2009. Direct organogenesis in petunia (Petunia hybrida Vilm.). Journal of Ornamental Horticulture, 12(1): 15-21.

Sharma AK, Mitra GC. 1976. In vitro culture of shoot apical meristem of Petunia hybrida for mass production of plants. Indian journal of experimental biology, 348-350.

Sharmin S A, Kabir A H, Mandal A, Sarker K K, Alam M F. 2008. In vitro propagation of eggplant through meristem culture. Agriculturae Conspectus Scientificus (ACS), 73(3): 149-155.

Sink K C. 1984. Taxonomy. In Petunia. Springer Berlin Heidelberg: (pp. 3-9)

Van der Krieken W M, Croes A F, Smulders MJ, Wullems GJ. 1990. Cytokinins and flower bud formation in Vitro in Tobacco role of the metabolites. Plant physiology, 92(3): 565-569.

Vidya SM, Krishna V, Manjunatha B K, Pradeepa M R. 2013. Micropropagation of Clerodendrum serratum L. through direct and indirect organogenesis. Plant Tissue Culture and Biotechnology, 22(2): 179-185.

Walla M, Elmaksood A, Fawzia A E, Hussein A B. 2016. In vitro Propagation of the Endangered Medicinal Plant Hyoscyamus muticus L. (Egyptian Henbane). J. Appl. Environ. Biol. Sci. 6(4): 25-34.

Wetherell DF. 1982. Introduction to in vitro propagation. Avery Pub Group.