Hibernation Period in Some Land Snail Species (Gastropoda: Helicidae): Epiphragmal Structure and Hypometabolic Behaviors

In this study, the hypometabolism behavior, epiphragm formation process, and the mineral composition of the epiphragmal structure of the Helix pomacella, Eobania vermiculata, Helix melanostoma, and Helix asemnis land snails were investigated during the hibernation period. 100 snails selected among mature individuals, 25 from each species, were collected from nature for the research. The snails were placed in four 50x50x5 cm pans containing 20-cm-deep humus soil. Snails were fed with lettuce by free feeding until the hibernation period. Hypometabolism behavior of snails that had hibernated between November and December 2018, and January and February 2019 were examined as entering the hibernation stage, hibernation stage, and leaving the hibernation stage. The ionic concentration (Calcium, Iron, and Phosphorus) of the epiphragm layer formed during the hibernation process was analyzed, its formation duration, and its degradation at the end of the hibernation period were examined. In the study, it was determined that the weight before hibernation reduced by 18-22% in all examined species (H. pomacella, E. vermiculata, H. melanostoma, and H. asemnis). The highest values in terms of epiphragm formation duration (19±0.30 days), weight (0.41±0.26 g), and thickness (0.310±0.22 mm) were determined in H. asemnis. In terms of the ionic concentration, the highest Calcium level was determined in H. asemnis (29.96±0.12 mg/g) while the highest Phosphorus level (4.02±0.20 mg/g) was determined in E. vermiculata, and the highest Iron content was found in H. pomacella (19.50±0.33 mg/g). The degradation duration of the epiphragm layer at the end of hibernation was determined the longest in E. vermiculata (7 days) whereas the shortest in H. pomacella (3 days). As a result of the present research, it was seen that the epiphragm layer is important for snails in terms of a sustainable life cycle and that the ecological tolerances of snails depend on the epiphragmal structure.

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Abdussamad MA, Olusegun AO, Olusiji FS, Samuel AO. 2010. Some haemolymph biophysical parameters in the giant African land snail Archachatina marginata during a six-week aestivation period. Global Veterinaria, 4(4): 400–408.

Ademolu KO, Idowu AB, Mafiana CF, Osinnowo OA. 2004. Performance, proximate and minerals analysis of African giant land snail (Archachatina marginata) fed differentnitrogen sources. African Journals of Biotechnology, 3(8): 412–414.

Ademolu KO, Fantola FO, Bamidele JA, Dedeka GA, Idowu AB. 2016. Formation and composition of epiphragm in three giant African land snails (Archachatina marginata,Achatina fulica and Achatina achatina), Ruthenica, 26(3-4): 165-169.

Adeyeye EI, Afolabi EO. 2004. Amino acid composition of three different types of land snails consumed in Nigeria. Food Chemistry, 85: 535-539.

Ajayi OA, Fawole JK, Idowu AB, Ademolu KO. 2012. Dynamics of nutrient in the tissues of giant African land snail (Archachatina marginata) during aestivation. 1st International Conference on giant African land snails [GALS]. Held at Federal University ofAgriculture, Abeokuta, Nigeria on 12th-15th February 2012, pp. 67–71.

Akinnusi O. 1998. A practical approach to backyard snail farming. Nigeria Journal of Animal Production, 25: 85-95.

Akinnusi O. 2014. Snail production and management. Tolukoya publishing company, Abeokuta, Nigeria, 105 p.

Akman MAA, Yazar S, Şahin İ, Yıldırım Z. 2005. Kayseri karpuz sekisi havzasında tatlı su gastropodlarının araştırılması. Sağlık Bilimleri Dergisi, 14(1): 1-5.

Arad Z. 2001. Desiccation and rehydration in land snails – a test for distinct set points in Thebapisana. Israel Journal of Zoology, 47: 41–53.

Barnes RD. 1987. Invertebrate Zoology 5th Edition. W. B. Saunders Company. p. 743.

Barnhart MC. 1983. Gas permeability of the epiphragm of a terrestrial snail, Otala lactea. Physiological Zoology, 56: 436–444.

Cook A. 2001. Behavioural ecology: On doing the right thing, in the right place at the righttime. InBarker, G. M. (Ed.): The Biology of Terrestrial Molluscs. CAB International. Wallingford. pp. 447-487.

Giokas S, Karkoulis P, Pafilis P, Valakos E. 2007. Relictual physiological ecology in the threatened land snail Codringtonia helenae: a cause for decline in a changing environment? Acta Oecologica, 32: 269–278.

Hermes-Lima M, Storey JM, Storey KB. 1998. Antioxidant defenses and metabolic depression. The hypothesis of preparation for oxidative stress in land snail. Comparative Biochemistry and Physiology B, 120: 437-448.

Kingsolver JG, Huey RB. 2008. Size, temperature, and fitness: three rules. Evolutionary Ecology Research, 10: 251–268.

Kingsolver JG. 2009. The well-temperatured biologist. (American Society of Naturalists Presidential Address). The American Naturalist, 174: 755–768.

Malcolm R, Liu C, Neilson RP, Hansen L, Hannah L. 2006. Global warming and extinction of endemic species from biodiversity hot spots. Conservation Biology, 20: 538–548.

Malleswar VNS, Basavaraju R, Krupanidhi S. 2013. Behavioral and Physiological Changes in Pila globosa (Indian Apple Snail) During Aestivation. Zoology, 2(8): 54-55.

Onadeko SA. 2010. Live weight changes and mortality rate in the giant african snail Archachatina marginata during six week aestivation period. World journal of Zoology,5(2): 75- 81.

Odiete WD. 1999. Environmental physiology of animals and pollution. Diversified Resources Ltd. Surulere, Lagos. 95-97.

Omoyakhi JM. 2007. Effect of aestivation on growth, bady composition and reproductive performance of Giant African Land Snails, Archachatina margiata and Achatinaachatina PhD thesis. University of Agriculture Abeokuta, Nigeria.

Porcel D, Bueno JD, Almendros A. 1996. Alterations in the digestive gland and shell of the snail Helix aspersa Müller (Gastropoda, Pulmonata) after prolonged starvation. Comparative Biochemistry and Physiology Part A: Physiology, 115(1): 11-17.

Prior DJ. 1985. Water regulatory behaviour in terrestrial gastropods. Biological reviews of theCambridge Philosophical Society, 60: 403–424.

Schütt H. 1993. Türkische Landschnecken, Prodromus faunae Anatolicae molluscorumterrestrium viventium testaceorumque, Vorläufige Zusammenstellung der aus Anatolienbekannt gewordenen gehäusetragenden Landschnecken, Türkiye'nin karasalsalyangozları, pp. 1-433. Wiesbaden. (Hemmen).

Storey KB. 2001. Molecular mechanisms of metabolic arrest: life in limbo. BIO Scientifcpublishers Ltd. Oxford UK, 216 pp.

Storey KB. 2002. Life in the slow lane, molecular mechanisms of estivation. ComparativeBiochemistry and Physiology - Part A, 134: 733–754.

Storey KB. 2007. Tribute to P. L. Lutz: putting life on 'pause'-- molecular regulation of hypo metabolism. Journal of Experimental Biology, 210(10): 1700-1714.

Struthers M, Rosair G, Buckman J, Vıney C. 2002. The Phyiıcal and Chemical Microstructureof the Achatına Fulica Epiphragm. Journal of Molluscan Studies, 68:165–171

Şereflişan H. 2020. Epifragma Mineral Bileşiminin ve Oluşum Sürecinin Dört Kara Salyangozu Türünde (Mollusca: Gastropoda: Helicidae) İncelenmesi. Çanakkale Onsekiz MartÜniversitesi Fen Bilimleri Enstitüsü Dergisi, 6(1): 105- 111.

Şereflişan H. 2019. Biyoçeşitlilik Noktasında Kara Salyangozunun (Helix Aspersa) HayattaKalma Direnci. 1st International Conference on Environment, Technology andManagement. 27-29 June/Niğde Halisdemir Üniversitesi, Environmental EngineeringDepartment/Niğde/ Turkey.

Welter-Schultes F. 2012. European non-marine molluscs, a guide for species identification.760 pages, ISBN 978-3-933922-75- 5.