SERPİL MİŞE YONAR, MUZAFFER MUSTAFA HARLIOĞLU

Effects of Dietary Selenium on Paraoxonase and Arylesterase Enzyme Activities in Freshwater Crayfish Astacus leptodactylus

In this study, the effects of selenium added to the diets of Astacus leptodactylus at different ratios on paraoxonase (PON) and arylesterase enzyme activities (ARE) in the hepatopancreas and gonad tissues were investigated. In this study, control (K), trial 1 (D1), trial 2 (D2) and trial 3 (D3) were prepared at the selenium levels of 0,3, 0,6, 0,9 and 1,2 mg/kg, respectively. The crude protein and total energy levels of experimental diets were equalized. 12 ponds in 2 × 2 × 1 m dimensions were used. 75 female and 25 male crayfish were stocked in each pond (totally 1200 crayfish). This study was carried out in triplicate. The crayfish were fed twice a day during 9 months. PON and ARE enzyme activities were investigated in the tissue samples taken monthly from the crayfish. During the trial, significant differences were observed in the PON and ARE enzyme activities in the hepatopancreas and gonad tissues. The PON and ARE enzyme activities increased statistically significant in the crayfish tissues during the breeding season and incubation period. This increase was found to be statistically different in the D1, D2 and D3 groups compared to the control group. In conclusion, selenium had positive effects on PON and ARE enzyme activities of A.leptodactylus during its mating, pleopodal egg laying and pleopodal egg carrying periods.

Kerevit (Astacus leptodactylus) Yemine Katılan Selenyumun Paraoksonaz ve Arilesteraz Enzim Aktivitelerine Etkisi

Bu çalışmada kerevit (Astacus leptodactylus Eschscholtz) yemine farklı oranlarda katılan selenyumun hepatopankreas ve gonad dokularında paraoksonaz (PON) ve arilesteraz (ARE) enzim aktivitelerine etkisi araştırılmıştır. Çalışmada, toplam selenyum düzeyi 0,3, 0,6, 0,9 ve 1,2 mg/kg yem olan sırasıyla; Kontrol (K), Deneme 1 (D1), Deneme 2 (D2) ve Deneme 3 (D3) yemleri oluşturulmuştur. Araştırma yemlerinin ham protein ve toplam enerji düzeyleri eşitlenmiştir. Çalışmada ebatları 2,0 × 2,0 × 1,0 m olan 12 adet havuz kullanılmıştır. Her bir havuza 75 dişi-25 erkek olacak şekilde toplam 1200 adet kerevit stoklanmıştır. Üç tekrarlı olarak yürütülen çalışmada kerevitler günde 2 öğün olmak üzere 9 ay süreyle yemlenmiştir. Kerevitlerden aylık olarak alınan doku örneklerinde PON ve ARE enzim aktiviteleri tespit edilmiştir. Deneme süresince hepatopankreas ve gonad dokularındaki PON ve ARE enzim aktivitelerinde istatistiksel olarak önemli farklılıklar bulunmuştur. Kerevitlerin dokularında üreme sezonu ve kuluçka süresi boyunca PON ve ARE enzim aktivitelerinin arttığı belirlenmiştir. Bu artışın kontrol grubuna kıyasla D1, D2 ve D3 gruplarında istatistiksel olarak farklı olduğu bulunmuştur. Sonuç olarak, selenyumun A. leptodactylus’un çiftleşme dönemi, yumurtlama dönemi ve kuluçka süresi boyunca PON ve ARE enzim aktivitelerini olumlu yönde etkilediği görülmüştür.

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