MEHMET YAMAN, ERCAN YILDIZ, Hasan PINAR, AYDIN UZUN, AHMET SÜMBÜL, Şule ALKAN

Determination of Genetic Diversity Using SRAP Technique in Some Walnut Genotypes Obtained by Selection

Turkey is among the few countries in terms of plant diversity. An example of this situation is the variation in walnut seedlings grown from seed throughout the country. The genetic material to be used in fruit breeding should be original and the genetic relations among the genotypes should be well elucidated. In this research, genetic diversity was investigated among the walnut genotypes selected from Uşak province of Turkey with the use of molecular markers. In this study, molecular results showed that the 19 SRAP primer combinations produced, on average, 171 bands in the accessions examined, of which 77.2% were polymorphic. Base lengths of the bands obtained from markers varied between 100-1500 bp. The similarity ratio of walnut genotypes varied between 0.21 – 0.85. According to the results of the study, it was determined that SRAP marker system can be used to determine genetic diversity among walnut genotypes.

Seleksiyonla Elde Edilmiş Bazı Ceviz Genotiplerinde SRAP Tekniği Kullanılarak Genetik Çeşitliliğin Belirlenmesi

Türkiye bitki çeşitliliği bakımından ender ülkeler arasındadır. Bu durumun bir örneği de ülke genelinde tohumdan yetişen ceviz çöğürlerindeki varyasyondur. Bu farklılığın ceviz ıslahında kullanılabilmesi için genetik materyalin ismine doğru olması ve aralarındaki genetik ilişkilerin belirlenmesi büyük önem taşımaktadır. Yapılan bu çalışmada Uşak ilinden seleksiyon ile elde edilmiş bazı ceviz genotiplerinde genetik çeşitliliğin moleküler markörler ile belirlenmesi amaçlanmıştır. Çalışmada 19 adet SRAP markör kombinasyonu kullanılmıştır. Bu markörlerden sayıları 2 ile 18 arasında değişen toplam 171 skorlanabilir bant elde edilmiş olup, bunların %77,2’si polimorfik olarak bulunmuştur. Markörlerden elde edilen bantların baz uzunlukları ise 100-1500 bp arasında değişim göstermiştir. Ceviz genotiplerine ait benzerlik oranı 0,21 – 0,85 arasında değişim göstermiştir. Çalışma sonucuna göre SRAP markör sisteminin ceviz genotipleri arasında genetik çeşitliliği belirlemede etkili şekilde kullanılabileceği tespit edilmiştir.

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Ahmad F, Akram A, Farman K, Abbas T, Bibi A, Khalid S, Waseem M. 2017. Molecular Markers and Marker Assisted Plant Breeding. Current Status and their Applications in Agricultural Development. Journal of Environmental and Agricultural Sciences, 11: 35-50.

Akcan S, Kafkas S, Sütyemez M, Akca Y, Eti S, Türemiş N. 2008. Kaman Cevizlerinde Apomiksis Olasılığının Dişi Çiçeklerin İzolasyonu ve Moleküler Yöntemlerle Araştırılması. Alatarım, 7 (1): 1-10.

Bayazit S, Kazan K, Gulbitti S, Cevic V, Ayanoglu H, Ergul A. 2007. AFLP analysis of genetic diversity in low chill requiring walnut (Juglans regia L.) genotypes from Hatay. Turkey Scientia Horticulturae, 111 (4): 394–398. doi: https://doi.org/10.1016/j.scienta.2006.11.006

Christopoulos MV, Rouskas D, Tsantili E, Bebeli P. 2010. Germplasm diversity and genetic relationships among walnut (Juglans regia L.) cultivars and Greek local selections revealed by Inter-Simple Sequence Repeat (ISSR) markers. Scientia Horticulturae 125 (4): 584–592. doi: https://doi.org/10.1016/j.scienta.2010.05.006

Cocen E, Pinar H, Uzun A, Yaman M, Saritepe Y, Kokargul R, Aslan A, Altun OK. 2020. Morphological and biochemical characteristics to be used in identification of mulberry genotypes. Fresenius Environmental Bulletin 29(1): 79-84.

Demir B, Sayıncı B, Çetin N, Yaman M, Çömlek R, Aydın Y, Sütyemez M. 2018. Elliptic Fourier based analysis and multivariate approaches for size and shape distinctions of walnut (Juglans regia L.) cultivars. Grasas y Aceites, 69(4): 271. doi: https://doi.org/10.3989/gya.0104181

Demir B, Yaman M, Çetin N. 2017. Tarımsal Biyokütle Enerjisine Hammadde Oluşturan Cevizin (Juglans Regia L.) Enerji Eşdeğeri”. Bahçe 46 (Özel Sayı 2): 83–87.

Dice LR. 1945. Measures of the amount of ecologic association between species, Ecology 26, 297-302. doi: https://doi.org/10.2307/1932409

Doğan Y, Kafkas S, Sütyemez M, Akça Y, Türemiş N. 2014. Assessment and characterization of genetic relationships of walnut (Juglans regia L.) genotypes by three types of molecular markers. Sci Hortic 168: 81–87.

Doğan Y. 2006. Bazı Ceviz (Juglans regia L.) Çeşit ve Genotiplerinin Moleküler Markör Teknikleri ile Karakterizasyonu Yüksek Lisans Tezi, Fen Bilimleri Enstitüsü, Çukurova Üniversitesi, Adana, Türkiye.

Doyle JJ, Doyle JL. 1990. Isolation of plant DNA from fresh tissue. Focus, 12: 13–15.

Ebrahimi A, Zarei A, Lawson S, Woeste KE, Smulders MJM. 2016. Genetic diversity and genetic structure of Persian walnut (Juglans regia) accessions from 14 European, African, and Asian countries using SSR markers. Tree Genetics and Genomes, 12:114. DOI: https://doi.org/10.1007/s11295-016-1075-y.

Ercisli S. 2004. A short review of the fruit germplasm resources of Turkey. Genetic Resources and Crop Evolution, 51(4): 419-435. doi: https://doi.org/10.1023/ B:GRES.0000023458. 60138.79

FAO, 2018. Food and Agriculture Organization of the United Nations. FAOSTAT, Available from: http://www.fao.org/ faostat/en/#data/QC [Accessed 14 April 2020]

Fjellstrom RG, Parfitt DE. 1994 Walnut (Juglans spp.) Genetic Diversity Determined by Restriction Fragment Length Polymorphisms. Genome, 37: 690-700. doi:https://doi.org/ 10.1139/g94-097

Fjellstrom RG, Parfitt DE. 1995 Phylogenetic Analysis and Evolution of Genus Juglans (Juglandaceae) as Determined from Nuclear Genome RFLPs. Plant Systematics and Evolution, 197: 19–32. doi: https://doi.org/10.1007/ BF00984629

Hayes D, Angove MJ, Tucci J, Dennis C. 2016. Walnuts (Juglans regia) chemical composition and research in human health. Critical Reviews in Food Science and Nutrition, 56(8): 1231- 1241. doi: 10.1080/10408398.2012.760516

Ipek M, Arıkan Ş, Pırlak L, Eşitken A. 2019. Phenological, Morphological and Molecular Characterization of Some Promising Walnut (Juglans regia L) Genotypes in Konya. Erwerbs-Obstbau, 61: 149–156. doi: https://doi.org/10.1007/ s10341-018-0411-9

Iqbal J. 2019. Morphological, physiological and molecular markers for the adaptation of wheat in drought condition. Asian Journal of Biotechnology and Genetic Engineering, 1- 13. DOI: 10.9734/AJBGE//2019/46253

Kafkas S, Özkan H, Sütyemez M. 2005. DNA Polymorphism and Assessment of Genetic Relatioships in Walnut Genotypes Based on AFLP and SAMPL Markers. Journal of the American Society for Horticultural Science 130(4): 585–590.

Kaya M. 2013. Uzundere’nin Cevizli Köyünde Yetişen Cevizlerin (Juglans spp.) Moleküler Karakterizasyonu. Yayınlanmamış Yüksek Lisans Tezi, Fen Bilimleri Enstitüsü, Atatürk Üniversitesi, Erzurum, Türkiye.

Malvolti ME, Spada M, Beritognolo I, Cannata F. 1997. Differentiantion of Walnut Hybrids (Juglans nigra L. x Juglans regia L.) through RAPD Markers. In: Gomez Pereira JA, Martins JMS, Pinto de Abreu C (editors). III International Walnut Congress, Alcobaça, Portugal, 13-16 June 1995, Acta Horticulturae 462, pp. 43–52. doi: 10.17660/ ActaHortic.1997.442.4.

Mantel N. 1967. The detection of disease clustering and generalized regression approach. Cancer Research, 27: 209–220. Mohammadi SA, Prasanna BM. 2003. Analysis of genetic diversity in crop plants-salient statistical tools and considerations. Crop Science, 43(4): 1235-1248. doi: https://doi.org/10.2135/cropsci2003.1235

Nicese FP, Hormaza JI, Mcgranahan GH. 1998. Molecular Characterization and Genetic Relatedness among Walnut (Juglans regia L.) Genotypes Based on RAPD Markers. Euphytica, 101: 199–206. doi: https://doi.org/10.1023/ A:1018390120142

Orman E. 2018. Bazı Yerli Ceviz (Juglans regia L.) Genotiplerinin Agromorfolojik ve Moleküler Olarak Tanımlanması. Yayınlanmamış Doktora Tezi, Fen Bilimleri Enstitüsü, Ege Üniversitesi, İzmir,Türkiye.

Öztürk Hİ, Dursun A, Hosseinpour A, Haliloğlu K. 2020. Genetic diversity of pinto and fresh bean (Phaseolus vulgaris L.) germplasm collected from Erzincan province of Turkey by inter-primer binding site (iPBS) retrotransposon markers. Turkish Journal of Agriculture and Forestry, vol.44, 417-427

Pınar H, Yaman M, Sarısu HC, Uzun A, Yiğit MA. 2018. Bazı Kiraz Anaçlarının Genetik Akrabalık İlişkilerinin RAPD Moleküler Markır Yöntemi ile Belirlenmesi. Harran Tarım ve Gıda Bilimleri Dergisi, 22(3): 326-334.

Pinar H, Ercişli S, Bircan M, Ünlü M, Uzun A, Yılmaz KU, Yaman M. 2017. Morphological, Molecular, and Self-(In) Compatibility Characteristics of New Promising Apricot Genotypes. Journal of Agricultural Science and Technology, 19: 365-376.

Pinar H, Uzun A, Unlu M, Yaman M. 2019. Genetic Diversity in Turkish Banana (Musa Cavendishii) Genotypes with Damd Markers. Fresenıus Environmental Bulletın, No.1, 459-463.

Powell W, Morgante M, Andre C, Hanafey M, Vogel J, Tingey S, Rafalski A. 1996. The Comparison of RFLP, RAPD, AFLP and SSR (microsatellite) Markers for Germplasm Analysis. Molecular Breeding, 2: 225–38. doi: https://doi.org/10.1007/ BF00564200

Rohlf JF. 2000. NTSYS-pc: Numerical Taxonomy and Multivariate Analysis System. Exeter Software, Setauket, New York.

Shah UN, Mir JI, Ahmed N, Fazili KM. 2016. Assessment of germplasm diversity and genetic relationships among walnut (Juglans regia L.) genotypes through microsatellite markers. Journal of the Saudi Society of Agricultural Sciences, 17(4):339-350. doi: https://doi.org/10.1016/j.jssas.2016.07. 005

Uzun A, Coşkun ÖF, Yaman M, Pınar H, Paris K. 2017. Identification of Genetic Similarities among Walnut (Juglans regia L.) Genotypes Selected from Central Anatolia Region of Turkey with SRAP Markers. Alatarım, 26-34.

Uzun A, Çil A, Yaman M, Coşkun ÖF. 2020 Genetic Diversity and Some Fruit Characteristics of Quince Genotypes Collected from Kayseri Region. Turkish Journal of Agriculture Food Science and Technology, 8(2): 318-323. doi: https://doi.org/10.24925/turjaf.v8i2.318-323.3012

Uzun A, Yesiloglu T, Aka-Kacar Y, Tuzcu O, Gulsen O. 2009. Genetic diversity and relationships within Citrus and related genera based on sequence related amplified polymorphism markers (SRAPs). Scientia Horticulturae 121 (3): 306–312. doi: https://doi.org/10.1016/j.scienta.2009.02.018

Woeste K, Mcgranahan GH, Bernatzky R. 1996. Randomly Amplified Polymorphic DNA Loci from A Walnut Backcross (Juglans hindsii x Juglans regia) x Juglans regia]. Journal of the American Society for Horticultural Science, 121(3): 358- 361.

Yaman M, Șekerci AD, Uzun A, Say A. 2017. Walnut production potential of Kayseri province. Bahçe, 46(2): 129-132.

Yıldız E, Okatan V, Çolak AM, Türk MU. 2017. Vegetative and Phenological Characteristics of Promising Walnut (Juglans regia L.) Genotypes in Uşak (Turkey) Province. The Eurasian Agriculture and Natural Sciences Congress, 20-23 September 2017. Bishkek, Kyrgyzstan, 18-24.

Yıldız E, Okatan V, Çolak AM, Türk MU. 2019. İç Ege Bölgesinden (Uşak) Seçilen Ümitvar Ceviz Genotiplerinin Tartılı Derecelendirme Yöntemine Göre Değerlendirilmesi. V. International Congress on Vocational and Technical Sciences. 24-27 January 2019, Cairo, Egypt, 73-78.

Yildiz E, Sumer G. 2019. Adaptation of Some Local and Foreign Walnut Cultivars (Juglans regia L.) under Inner Aegean Region/Turkey Ecological Conditions. Fresenius Environmental Bulletin, 28(7): 5206-5211.

Zhao H, Bai H, Jing Y, Li W, Yin S, Zhou H. 2017 A pair of taxifolin-3-O-arabinofuranoside isomers from Juglans regia L. Natural Product Research, 31(8): 945-950. doi: https://doi.org/10.1080/14786419.2016.1258554