The purpose of this study was to investigate the possible presence of sesame in commercial foods normally carrying no warning for the allergen, but which may have been subjected to contamination during processing. One hundred units of widely consumed goods with high potential to contain allergenic substances deriving from nuts were analyzed, using sensitive and capable PCR (C-PCR) and Real Time PCR (RT-PCR) methodologies. Of the products examined, 15 (15.0%) declared the presence of sesame, 36 (36.0%) carried no food allergy label, 44 (44.0%) were marked by the phrase may contain traces of nuts and 5 (5.0%) carried the indication may contain sesame traces. The sesame-positive products detected using the C-PCR method were 15 (100%), 12 (33.3%), 14 (31.8%) and 3 (60%), respectively. Using the RT-PCR technique, positive results were obtained for 15 (100%), 18 (50.0%), 18 (20.5%) and 5 (100%) samples, respectively. The results indicate that the PCR methods applied are highly sensitive and selective, which makes them suitable for the detection of sesame traces in food samples. In addition, they can be useful for monitoring the effectiveness of cleaning processes in the production units of the food industry.
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Beyer K, Bardina L, Grishina G, Sampson HA. 2002. Identification of sesame seed allergens by 2-dimensional proteomics and Edman sequencing: Seed storage proteins are common food allergens. J Allergy Clin Immun. 110: 154-159.
Beyer K, Grishina G, Bardina L, Sampson HA. 2007. Identification of 2 new sesame seed allergens: Ses i 6 and Ses i 7. J Allergy Clin Immun. 119: 1554-1556.
Brzezinski JL.2007. Detection of sesame seed DNA in foods using real-time PCR. J Food Protect. 70: 1033-1036. Cohen A, Goldberg M, Levy B, Leshno M, Katz Y. 2007.
Sesame food allergy and sensitization in children: the natural history and long-term follow-up. Pediatr Allergy Immu. 18: 217-223.
Crespo JF, James JM, Fernandez-Rodriguez C, Rodriguez. 2006. J. Food allergy: nuts and tree nuts. Brit J Nutr. 96: 95- 102.
Holzhauser T, Vieths S. 1999. Indirect competitive ELISA for determination of traces of peanut (Arachis hypogaea L) protein in complex food matrices. J Agric Food Chem. 47: 603-611.
Holzhauser T, Wangorsch A, Vieths S. 2000. Polymerase chain reaction (PCR) for detection of potentially allergenic hazelnut residues in complex food matrixes. Eur Food Res Technol. 211: 360-365.
Kanu PJ, Zhu K, Kanu JB, Zhou H, Qian H, Zhu K. 2007. Biologically active components and nutraceuticals in sesame and related products: a review. Trends Food Sci Tech. 18: 599-608.
Koppel R, Dvorak V, Zimmerli F, Breitenmoser A, Eugster A, Waiblinger H-U. 2010. Two tetraplex real-time PCR for the detection and quantification of DNA from eight allergens in food. Eur Food Res Technol. 230: 367-374.
Koppelman SJ, Knulst AC, Koers WJ, Penninks AH, Peppelman H, Vlooswijk R, Pigmans I, van Duijn G, Hessing M. 1999.
Comparison of different immunochemical methods for the detection and quantification of hazelnut proteins in food products. J Immunol Methods. 229: 107-120.
Leduc V, Moneret-Vautrin DA, Tzen JTC, Morisset M, Guerin L, Kanny G. 2006. Identification of oleosins as major allergens in sesame seed allergic patients. Allergy. 61: 349- 356.
Mustorp S, Engdahl-Axelsson C, Svensson U, Holck A. 2008. Detection of celery (Apinum graveolens), mustard (Sinapis alba, Brassica juncea, Brassica nigra) and sesame (Sesamum indicum) in food by real-time PCR. Eur Food Res Technol. 220: 771-778.
Pastorello EA, Varin E, Farioli L, Pravettoni V, Ortolani C, Trambaioli C, Fortunato D, Giuffrida MG, Rivolta F, Robino A, Calamari AM, Lacava L, Conti A. 2001. The major allergen of sesame seeds (Sesamum indicum) is a 2S albumin. J Chromatogr B. 756: 85-93.
Pedersen MH, Holzhauser T, Bisson C, Conti A, Jensen LB, Skov PS, Bindslev-Jensen C, Brinch DS, Poulsen LK. 2008. Soybean allergen detection methods-A comparison study. Mol Nutr Food Res. 52: 1486-1496.
Perkins MS. 2001.Raising awarness of sesame allergy. Pharm J. 267: 757-758.
Poms RE, Klein CL, Anklam E. 2004. Methods for allergen analysis in food: a review. Food Addit Contam. 21: 1-31.
Sampson HA. Uptake on food allergy. 2004. J Allergy Clin Immunol. 113: 805-819.
Schoringhumer K, Cichna-Markl M. 2007. Development of a Real-Time PCR method to detect potentially allergenic sesame (Sesamum indicum) in food. J Agric Food Chem. 55: 10540-10547.
Schoringumer K, Redl G, Cichna-Markl M. 2009. Development and Validation of a Duplex Real-Time PCR Method to Simultaneously Detect Potentially Allergenic Sesame and Hazelnut in Food. J. Agric. Food Chem. 57: 2126-2134.
Wolff N, Cogan U, Admon A, Dalal I, Katz Y, Hodos N, Karin N, Yannai S. 2003. Allergy to sesame in human is associated primarily with IgE antibodyto a 14 kDa 2S albumin precursor. Food Chem Toxicol. 41: 1165-1174.
Yeung JM, Collins PG. 1996. Enzyme immunoassay for determination of peanut proteins in food products. JAOAC. 79: 1411-1416.