FATİH MEHMET GÜR, SEMA TİMURKAAN, SAİME BETÜL BAYGELDİ, ZAİT ENDER ÖZKAN, BERRİN TARAKÇI GENÇER, YEŞİM ASLAN KANMAZ, RAMAZAN İLGÜN, Hatice Emel GÜR

Cüce Hamsterlerin (Phodopus roborovskii) Genital Dokularında Androjen Reseptör Lokalizasyonu

Androjenler hedef dokulardaki etkilerini esas olarak androjen reseptör (AR) aracılığıyla gösterirler. Bu çalışmada dişi ve erkek cüce hamster genital dokularında AR lokalizasyonu araştırıldı. Mevcut çalışmada 6 adet dişi, 6 adet erkek hayvan kullanıldı. Anestezi sonrası, çalışılacak dokular, hızlı bir şekilde vücuttan uzaklaştırılarak %10’luk tamponlu nötral formalinde tespitin ardından parafine gömüldü. Kesitler, mikrodalga ışınımlı “antijen retrieval” tekniği uygulandıktan sonra immunohistokimyasal yöntemlerle boyandı. AR pozitif immunboyanma testis, kaput epididimis, prostat bezi ve vezikula seminalis dokularında yalnızca hücre çekirdeklerinde gözlenirken, ovaryum ve ovidukt dokularında hücrelerin bir kısmında sitoplazmada bir kısmında ise çekirdekte gözlendi. Cüce hamster erkek ve dişi genital dokularındaki AR lokalizasyonu, diğer türlerle benzerdi. Genital dokularda AR varlığı, androjenlerin bu dokular için elzem olduğu görüşünü desteklemektedir.

Androgen Receptor Localization in the Genital Tissues of the Dwarf Hamsters (Phodopus roborovskii)

Androgens perform the their effects on target tissues mainly through the androgen receptor (AR). In this study, AR localization was investigated in female and male dwarf hamsters genital tissues. In the present study, 6 female and 6 male animals were used. After anesthesia, the tissues to be worked were quickly removed from the body and fixed in 10% buffered neutral formalin, and embedded in paraffin. Sections were stained after microwave antigen retrieval for immunohistochemistry. Although AR positive immunostaining was observed only in cell nuclei in testis, caput epididymis, prostate gland and vesicle seminalis tissues, it was observed in cytoplasm or nucleus of the cells in the ovarium and oviduct tissues. AR localization in dwarf hamster male and female genital tissues was similar to other species. The presence of AR in genital tissues supports the idea that androgens are essential for these tissues.

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