Nezih ATA, Onur YILMAZ, Yasemin ÖNER, Seyrani KONCAGÜL, Candan ERİŞ, Cihan ÜNAL

Beş Yerli Türk Sığır Irkında GH-MspI and IGF1-SnaBI Lokuslarındaki Polimorfizmlerin Analizi

Büyüme Hormonu (GH) ve İnsülin Benzeri Büyüme Faktörü-I (IGF-I) somatotropin axis yolağının üyeleridirler. Doğum sonrası gelişim, hücre farklılaşması ve metabolizmada gibi mekanizmalarda anahtar rol oynarlar. Hayati önemlerinden dolayı bunları kodlayan genlerin anlaşılmaları da gereklidir. Bu çalışmada beş yerli sığır ırkı (Yerli Güney Sarısı (YGS), Güneydoğu Anadolu Kırmızısı (GAK), Boz Irk (BI), Yerli Kara (YK) ve Doğu Anadolu Kırmızısı (DAK) GH-MspI ve IGF1-SnaBI locusları bakımından PCR-RFLP yöntemi ile incelenmiştir. GH-MspI lokusu için 198, IGF1-SnaBI lokusu için 194 örnek analiz edilmiştir. Her iki lokusta iki allel ve üç genotip gözlenmştir. GH-MspI ve IGF1- SnaBI lokusları için predominant alleler sırasıyla A ve B olarak bulunmuştur. GH-MspI ve IGF1-SnaBI locusları için A ve B allelerinin frekansları sırasıyla 0,400-0,875 ve 0,846-0,903 arasında hesaplanmıştır. İncelenen populasyonlardan sadece DAK IGF1- SnaBI bakımından Hardy-Weinberg dengesindeyken, GH-MspI lokusu için sadece GAK populasyonunda Hardy-Weinberg dengesinden sapma gözlenmiştir.

Analysis of Polymorphisms on GH-MspI and IGF1-SnaBI Loci in Five Turkish Native Cattle Breeds

Growth Hormone (GH) and Insulin like Growth Factor-I (IGF1) are members ofsomototrophine axis pathway. They play a role in key on several mechanisms such aspostnatal growth, cell differentiation and metabolism. Due to their vital importance,polymorphisms on the genes coding are worth to be understood. In this study five nativecattle breeds (Native Southern Yellow (NSY), South Anatolian Red (SAR), AnatolianGrey (AG), Native Black (NB), East Anatolian Red (EAR) were investigated by PCRRFLPmethod for GH-MspI and IGF1-SnaBI loci. 198 and 194 samples were analyzedfor GH-MspI and IGF1-SnaBI loci, respectively. In both two loci two alleles and threegenotypes were observed. Predominant alleles were A and B for GH-MspI locus IGF1-SnaBI loci, respectively. Frequencies of A and B alleles were calculated between 0.400-0.875 and 0.846-0.903 for GH-MspI locus IGF1-SnaBI loci, respectively. While amonginvestigated population only EAR population was at Hardy-Weinberg equilibrium forIGF1-SnaBI locus, for GH-MspI only, in SAR population no deviation from Hardy-Weinberg equilibrium.

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