Antilisterial Activity by Enterococcus Species Isolated from Traditional Cheeses

In this study, it was aimed to detect the antimicrobial activity of 312 Enterococcus species against Listeria monocytogenes. Antimicrobial activity was detected by agar spot and well diffusion assay. A total of 201 enterococcal strains inhibited the growth of L. monocytogenes strains based on the agar spot test. Only 44 strains showed antimicrobial activity against L. monocytogenes strains using agar well diffusion assay. Of the 44 enterococcal strains screened, 6 E. faecium (2.99%) strains had a high antimicrobial effect against indicator L. monocytogenes strains. The antilisterial activity of 6 E. faecium strains had lost after treatment of proteinase K, trypsin and pepsin. The antimicrobial compounds of these strains could be a protein or peptides nature. E. faecium strains were more active against L. monocytogenes than E. faecalis strains.

Geleneksel Peynirlerden İzole Edilen Enterococcus Türlerinin Antilisterial Aktivitesi

Bu çalışmada, 312 adet Enterococcus suşunun Listeria monocytogenes’e karşı antimikrobiyel aktivitesinin saptanması amaçlanmıştır. Antimikrobiyel aktivite agar spot test ve kuyu difüzyon testleri ile tanımlanmıştır. Agar spot test sonucunda toplamda 201 enterokokkal suş, L. monocytogenes gelişimini inhibe etmiştir. Kuyu difüzyon testi sonucunda sadece 44 suş gıda kaynaklı patojene karşı antimikrobiyel aktivite göstermiştir. Taraması yapılan 44 suş içinden, 6 tanesi (%2,99) indikatör suşa karşı yüksek antimikrobiyel etki göstermiştir. Proteinaz K, tripsin ve pepsin uygulamaları sonucunda, 6 E. faecium suşunun antilisterial aktivitesi kaybolmuştur. Bu suşlardaki antimikrobiyel aktivitenin doğası protein veya peptit yapısındaki antimikrobiyel bileşiklerden kaynaklanabilir. E. faecium suşları, E. faecalis suşlarına oranla indikatör suşlara karşı daha fazla aktivite göstermiştir.

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Türk Tarım - Gıda Bilim ve Teknoloji dergisi-Cover
  • ISSN: 2148-127X
  • Yayın Aralığı: 12
  • Başlangıç: 2013
  • Yayıncı: Turkish Science and Technology Publishing (TURSTEP)
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