Sume BEGUM, Md. SALAUDDİ, Khaled HOSSAİN, Deloara BEGUM

Antibiogram Study of Bacterial Pathogen from Tilapia Fish in Bangladesh

Bacterial pathogens are isolated, identified and antibiogram were performed by taking the skin, gills and intestine of twenty randomly selected Tilapia fish (Oreochromis niloticus) that collected from local market of the Dinajpur city, Bangladesh. A serial dilution was prepared with the stated sample and at the amount of 0.1 ml was plated on nutrient agar, differential also specific media respectively. Then gram’s staining, colony morphology, biochemical test and antibiogram performed respectively. The four different isolated species with frequency of occurrence are 31(40.26%) Escherichia coli, 3 (29.87%) Staphylococcus spp., 13 (16.88%) Pseudomonas spp., 10 (12.99%) Salmonella spp. respectively. Some of these pathogens have tendency to transmit to man, who eat fish or deal with fish and fish products. Amoxicillin, Cefixime, Azithromycin, Chloramphenicol, Ciprofloxacin, Penicillin G, Erythromycin, Vancomycin, Gentamicin and Neomycin antibiotics was performed during sensitivity test. Among the total (77) isolated bacteria were sensitive to gentamycin, chloramphenicol, ciprofloxacin and Azithromycin but resistant to Amoxicillin, Penicillin G, Vancomycin and Erythromycin. The study was conducted in term of medical importance. Hence it is considered that a variety of bacterial species can be associated with fresh Tilapia fish related pathogen to humans.

PDF

___

Abou El-Atta ME. 2003. Efficiency of polymerase chain Reaction (P.C.R.) in diagnosis of some bacterial fish pathogens. Ph.D. Thesis in Vet. Sci (Bacteriology, immunology and mycology), pp. 44-63.

Adam AJ, Tobaias WJ. 1999. Red Mang rove proproot habitat as a finfish nursery area; a case study of salt rivea bay, st. Croix, USVI. Proc Gulf Caribb fish inst, 46: 22-46.

Al-Harbi AH, Uddin N. 2003. Quantitative and qualitative studies on bacterial flora of hybrid tilapia (Oreochromis niloticus and O. aureus) cultured in earthen ponds in Saudi Arabia. Aquaculture Research, 34: 43 - 48.

Allen DA, Austin B, Colwell RR. 1983. Numerical taxonomy of bacterial isolates associated with of freshwater fishery. Journal of general microbiology, 129: 2043-2062.

Aly SEM. 1994. Pathological studies on some fish in Suez Canal area. Ph.D. Thesis (pathology) Fact. of Vet. Med. Suez. Canal University.

Angka SL, Lioe KG. 1982. Control of bacterial hemorrhagic septicaemia in cultured fish. Bul. Perel Inst. Pertan Bogor, 3(3): 13-14.

Austin B, Al-Zahrani AMJ. 1988. The effect of antimicrobial compounds on the gastrointestinal microflora of rainbow trout. Journal Fish Biology, 33: 1-14.

Belton B, Karim M, Thilsted S, Murshed-E-Jahan K, Collis W, Phillips M. 2011. Studies and Reviews 2011-53. The World Fish Center. Penang, Malaysia. p. 71.

Cabello FC. 2006. Heavy use of prophylactic antibiotics in aquaculture: a growing problem for human health and for the environment. Environmental Microbiology, 8 (7): 1137- 1144.

Cahill MM. 1990. Bacterial flora of fishes: a review. Microbiology Ecology, 19(1): 21-41.

Cappuccino JG, Sherman N. 1996. Microbiology- A Laboratory Manual. 4th edn. California: The Benjamin/Cummings Publishing Co., Inc.

Carter GR. 1979. Diagnostic Procedures in Veterinary Bacteriology and Mycoplasma. 3rd edn. Charles C, Thomas Publicher U.S.A., pp. 398-417.

Chen HY, Kou GH. 1987. Studies on bacterial drug resistance in aquaculture I Drug resistance of bacteria in pond reared eels (Anguilla japonica). The Memoir of Bacteriology and Immunology in Fish Disease, 10: 12-24.

Chonoko U, Sule E, Ado A. 2014. Antibiotic susceptibility of pyogenic streptococci in catfish and tilapia from fresh water demonstration fish ponds, mando, kaduna. Continental J. Pharmaceutical Sciences, Wilolud Journals, 8(1): 7-15.

Douglas D. 2007. Identifying fresh water Aquarium fish disease.

Eissa IAM, Badran AF, Diab AS, Abdel-Rahman AM. 1996. “Some studies on prevailing bacterial diseases among cultured tilapias in Abbassa fish farms”. Proceedings of the 3rd Vet. Med. Congress, Zagazig University, Egypt, pp. 185- 202.

Emikpe BO, Adebisi T, Adedeji OB. 2011. Bacteria load on the skin and stomach of Clarias Gariepinus and Oreochromis Niloticus from Ibadan, South West Nigeria: Public health implications. Journal of Microbiology and Biotechnology Research, 1: 52-59.

EUCAST. 2015. Breakpoint tables for interpretation of MICs & zone diameters, version 5.0.

FAO. 2007. Food and Agriculture Organization of WHO. The state of the world Fisheries and Aquaculture.

FRSS. 2012. A Comprehensive Study on High Value Fish (Tilapia, Pangas and Koi) in Bangladesh.

Gerald PB, Ricardo BV, Fainstein LJ. 1983. Infections caused by Pseudomonas aeruginosa. Reviews of Infectious Diseases, 5 (2): 279-313.

Goncalves JR, Braum G, Fernandes A, Biscaia I, Bastardo J. 1992. Aeromonas hydrophila fulminate pneumonia in a fit young man Tborax, 47: 482-483.

Guzman MC, Bistoni MA, Tamagnini LM, Gonzalez RD. 2004. Recovery of Escherichia coli in fresh water fish, Jenynsia multidentata and Bryconamericus iheringi. Water Research, 38: 2368–2374.

Hnadi GBB. 2008. Microbial contaminations of some fresh water fish species (Oreochromis niloticus, Clarias spp and Synodontis spp). B.Sc. (Hon) Department of fisheries, University of Juba.

Jawahar AT. 2011. Food safety hazards related to emerging antibiotic resistant bacteria in cultured Freshwater fishes of Kolkata, India. Advance Journal of Food Science and Technology, 3 (1): 69-72.

Kaneko S. 1971. Microbiological study of fresh fish. New Food Industry, 13: 76–80.

Kar D, Michael RG, Kar S, Changkija S. 1990. Studies on fish epidemics from Assam. Journal of Indian Fish Association, 20: 73-75.

Karki H, Mustafa A, Master AL, Dhawale S. 2013. Antibiotic Resistant Bacteria in the Gut of Hatchery-reared Tilapia and Coho Salmon. Universal Journal of Microbiology Research, 1(3): 43-46.

Kasing A, Asiah MY, Kumbang J. 1999. Distribution of bacteria in tropical freshwater fish and ponds. International Journal of Environmental Health Research, 9 (4): 285-292.

Khan SM, Nunjurul H, Shafi M. 1987. Study of the bacteriological flora in the alimentary canal of Clarias batrachus and Heteropneustes fossilis in Bangladesh. Bangladesh Association for the Advancement of Science, Dhaka (Bangladesh). Proceedings of the 12th Annual Bangladesh Science Conference, pp. 35-36.

Ladipo O, Fabiyi and Fatula GT. 1981. Marketing and distribution of fish in Nigeria. Report submitted to the federal development of fisheries, Lagos. p. 35.

Leisner JJ, Vancanneyt M, Rusul G, Pot B, Lefebvre K, Fresi A, Tee LT. 2001. Identification of lactic acid bacteria constituting the predom, nating microflora in an acid feamented condiment (tempoyak) popular in Malaysia. International Journal of food microbiology, 63: 147-157.

Mhango M, Mpuchane SF, Gashe BA. 2010. Incidence of indicator organisms, opportunistic and pathogenic bacteria in fish. African Journal of Food, Agriculture, Nutrition and Development, 10(6): 4202-4218.

Mohamed HA, Lakshmanaperumalsamy P. 1997. Prevalence of Salmonella in fish and crustaceans from markets in Coimbatore, South India. Food Micro., 14(2): 111-116.

Mohammed XSA. 1999. Quality monitoring of some farm in Kafr Elsheikh. Association of Medical Journal, 41: 152- 161.

Roberts RJ, Horne MT. 1978. Bacterial meningitis in farmed rainbow trout salmogarirdneri Richrdson, affected with chronic pancreatic necrosis. Journal of fish diseases, 1: 157- 164.

Romana-Eguia MRR, Ikeda M, Basiao ZU, Taniguchi N. 2004. Genetic diversity in farmed Asian Nile and red hybrid tilapia stocks evaluated from microsatellite and mitochondrial DNA analysis. Aquaculture, 236:131-150.

Ryser ET, Marth EH, Taylor SL. 1984. Histamine production by psychrotrophic pseudomonads isolated from fish. Journal of Food Protection, 47: 378-380.

Shirak A, Cohen-Zinder M, Barroso RM, Seroussi E, Ron M, Hulata G. 2009. DNA bar-coding of Israeli indigenous and introduced cichlids. Israeli Journal of Aquaculture, 61: 83-88.

Stoskopf MK. 1993. Zoonotic diseases. In: Fish medicine. W. B. Saunders Company. pp. 214- 217.

Tripathy S, Kumar N, Mohanty S, Samanta M, Mandal RN, Maiti NK. 2007. Characterization of Pseudomonas aeruginosa isolated from freshwater culture systems. Microbiological Research, 162 (4): 391-396

Vogt RL, Dippold L. 2005. Escherichia coli O157:H7 outbreak associated with consumption of ground beef. Public Health Reports, 2: 174–178.

Wakabayashi H, Egusa S. 1972. Characteristics of Pseudomonas spp. from an epizootic of pond-cultured eels. Bull. Japan Soc. Scient. Fish, 38: 577-587.

Weinstein MR, Litt M, Kertesz DA, Wyper P, Rose D, Coulter M, McGeer A, Facklam R, Ostach C, Willey BM, Borczyk A, Low DE. 1997. Invasive infections due to a fish pathogen, Streptococcus iniae. England Journal of Medicine, 337: 589- 594.

WHO. 2007. Food safety issues associated with products from aquaculture. T.R.S. No. 883, World Health Organization, Geneva, Switzerland.

Zlotkin A, Chilmonczyk S, Eyngor M, Ghittino C, Hurvitz A. 2003. Trojan horse effect: phagocyte-mediated Streptococcus iniae infection of fish. Infect Immun, 71 (5): 2318-2325.