Alfa-amilaz Enzimlerini Üreten Termofilik Bacillus Suşlarının İzolasyonu ve Enzimlerin Kısmi Karakterizasyonu

Bu çalışmada, Erzin sınırları içerisinde bulunan Burnaz Çayı kıyı kesimlerinden toplanan toprak numunelerinden üç adet termofilik Bacillus sp. izolasyonu gerçekleştirilmiştir. Bakteriler sırasıyla Bacillus sp. CT1, CT2 ve CT3 olarak isimlendirilmişlerdir. Bakterilerce α-amilaz üretimi CT1 suşu için 60°C, CT2 ve CT3 suşları için ise 80°Cde maksimum düzeye çıkmıştır. CT1 α-amilazı optimum aktivitesini 90°C, CT2 ve CT3 α- amilazları ise 60°Cde göstermişlerdir. Yine CT2 α-amilazı optimum aktivitesini pH 7,0da, CT1 ve CT3 α-amilazları ise pH 8,0da göstermişlerdir. CT1, CT2 ve CT3 enzimlerine ait spesifik aktiviteler 55°Cde sırasıyla 317,6; 113,3 ve 362,7 U/mg olarak gerçekleşmiştir. Enzimlerin moleküler ağırlıkları zimogram analizi ile CT1 ve CT3 α- amilazları için 65 kDa, CT2 α-amilazı için ise 38 kDa olarak hesaplanmıştır.

Isolation of Alpha-amylase Producing Thermophilic Bacillus Strains and Partial Characterization of the Enzymes

In the present study, we isolated three thermophilic Bacillus strains from the soil samples collected from the coast sediments of the Burnaz Stream located in Erzin. The isolates were entitled as Bacillus sp. CT1, CT2, and CT3, respectively. The maximum α-amylase production was revealed at 60°C for CT1 strain, and at 80°C for CT2 and CT3 strains, respectively. The optimum enzyme activity was observed at 90°C for CT1 α-amylase, whereas at 60°C for CT2 and CT3 α-amylases. On the other hand, optimum pH value for CT2 α-amylase was 7.0, whereas 8.0 for CT1 and CT3 α-amylases. The specific activities of CT1, CT2, and CT3 amylases were 317.6, 113.3 and 362.7 U/mg at 55°C, respectively. The estimated molecular weight of CT1 and CT3 α-amylase was 65 kDa, and for CT2 α-amylase was 38 kDa by zymogram analysis.

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