Deniz ULUIŞIK, Durmuş HATİPOĞLU, Ercan KESKİN

Effects of Curcumin on Hematological Parameters in Aflatoxin B1 Applied Rats

In this study, it is aimed to determine the possible effects of curcumin on hematological parameters in aflatoxin appliedrats. Thirty eight healthy male Wistar Albino rats were used in the study. Group I animals was no applied. Animals in Group IIwere orally given 1 ml 10% DMSO daily for 60 days. Animals in Group III were orally given 300 mg/kg curcumin dissolved in10% DMSO daily for 60 days. Animals in Group IV were orally given 250 µg/kg aflatoxin B1 dissolved in 10% DMSO daily for60 days. Animals in Group V was orally given 250 µg/kg aflatoxin B1 dissolved in 10% DMSO and 300 mg/kg curcumindissolved in 10% DMSO daily for 60 days. At the end of the study, erythrocyte count, leukocyte count, platelet count,hemoglobin amount, hematocrit value, percentage ratios of leukocyte types, mean corpuscular volume (MCV), meancorpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) were determined in blood samplestaken from all animals. In the study, erythrocytes count, hemoglobin concentration, hematocrit value and MCHC level in theaflatoxin applied rats significantly decreased compared to the control group (p

PDF

___

1. Abbès S, Ben Salah-Abbès J, Abdel-Wahhab MA, Ouslati R. Immunotoxicological and biochemical effects of Aflatoxins in rats prevented by Tunisian Montmorillonite with reference to HSCAS. Immunopharmacol Immunotoxicol, 2010; 32(3): 514- 522.
2. Abd Allah OA, Fararh KM, Farid AS, Gad FA. Hematological and hemostatic changes in aflatoxin, curcumin plus aflatoxin and curcumin treated rat. Benha Vet Med J, 2017; 32(2): 151- 156.
3. Abdelrachied HG. Haematological parameters in rats treated with royal jelly and curcumin antioxidants after CCL4haemotoxicity. J Adv Biol, 2015; 7(1): 1214-1219.
4. Abdel-Rahman TM, Ali DMI, Abo-hagger AA, Ahmed MS. Efficacy of banana peel in reduction of aflatoxin toxicity in rats. J Agric Chem Biotechn, Mansoura Univ, 2017; 8(10): 251- 259.
5. Abdel-Wahhab MA, Nada SA, Khalil FA. Physiological and toxicological responses in rats fed aflatoxin-contaminated diet with or without sorbent materials. Anim Feed Sci Technol, 2002; 97(3-4): 209-219.
6. Abdel-Wahhab KG, Mannaa FA, Abdel-Wahhab MA. Panax ginseng C.A. Meyer extract protects rat erythrocyte from the oxidative damage induced by the synergistic effects of subchronic treatment with aflatoxin B1 and fumonisin. British J Med Medic Res, 2014; 4(9): 1883-1901.
7. Abeena B, George AJ, Mohammed Shejir R, Nair ND, Manomohan CB. Haematobiochemical changes of layer chicken in experimental Aflatoxicosis. Indian Vet J, 2015; 92(10): 84-86.
8. Al-Saad LA, Al-Badran AI, Al-Jumayli SA. Extra cellular ability of bacillus subtilis and psedomonas fluoresence to degrade aflatoxin B1 produced by aspergillus flavus. World J Pharmaceut Res, 2015; 4(8): 157-173.
9. Antony S, Kuttan R, Kuttan G. Imunomodulatory activity of curcumin. Immunol Invest, 1999; 28(5-6): 291-303.
10. Banji D, Pinnapureddy J, Banji OJ, Kumar AR, Reddy KN. Evaluation of the concomitant use of methotrexate and curcumin on Freund's complete adjuvant-induced arthritis and hematological indices in rats. Ind J Pharmacol, 2011; 43(5): 546-550.
11. Biehl ML, Buck WB. Chemical contaminants: Their metabolism and their residues. J Food Protect, 1987; 50(12): 1058-1073.
12. Brameld JM, Parr T. Improving efficiency in meat production. Proc Nutr Soc, 2016; 75(3): 242-246.
13. Chang CF, Hamilton PB. Refractory phagocytosis by chicken thrombocytes during aflatoxicosis. Poult Sci, 1979; 58(3), 559- 561.
14. Cloherty EK, Livine KB, Carruthers A. The red blood cell glucose transporter presents multiple nucleotide sensitive sugar exit sites. Biochemistry, 2001; 40(51): 15549-15561.
15. Dall’Asta C, Mangia M, Berthiller F, Molinelli A, Sulyok M, Schuhmacher R, Krska R, Galaverna G, Dossena A, Marchelli R. Difficulties in fumonisin determination: the issue of hidden fumonisins. Anal Bional Chem, 2009; 395(5): 1335-1345.
16. Di Gregorio MC, Bordin K, Souto PCMC, Corassin CH, Oliveira CAF. Comparative biotransformation of aflatoxin B1 in swine, domestic fowls, and humans. Toxin Rev, 2015; 34(3): 142-150.
17. Gandhi P, Khan Z, Chakraverty N. Soluble curcumin: A promising oral supplement for health management. J Appl Pharmaceutical Sci, 2011; 1(2): 1-7.
18. Gowda NK, Ledoux DR, Rottinghaus GE, Bermudez AJ, Chen YC. Efficacy of turmeric (Curcuma longa), containing a known level of curcumin, and a hydrated sodium calcium aluminosilicate to ameliorate the adverse effects of aflatoxin in broiler chicks. Poult Sci, 2008; 87(6): 1125-1130.
19. Guerre P. Worldwide mycotoxins exposure in pig and poultry feed formulations. Toxins, 2016; 8(12): 350.
20. Harvey RB, Kubena LF, Phillips TD, Corrier DE, Elissalde MH, Huff WE. Diminution of aflatoxin toxicity to growing lambs by dietary supplementation with hydrated sodium calcium aluminosilicate. Am J Vet Res, 1991; 52(1): 152-156.
21. Irene II, Onyechi O. Effect of dietary incorporation of vernonia amygdalina. Del on AFB1 induced hepatotoxicity in weanling albino rats. Jamaican J Sci Technol, 2004; 15: 32-36.
22. Kalpravidh RW, Siritanaratkul N, Insain P, Charoensakdi R, Panichkul N, Hatairaktham S, Srichairatanakool S, Phisalaphong C, Rachmilewitz E, Fucharoen S. Improvement in oxidative stress and antioxidant parameters in betathalassemia/Hb E patients treated with curcuminoids. Clin Biochem, 2010; 43(4-5): 424-429.
23. Kaneko JJ. Clinical Chemistry of Domestic Animals (4th ed.). San Diego, CA: Academic Press, 1989.
24. Kubena LF, Harvey RB, Huff WE, Elissalde MH, Yersin AG, Phillips TD, Rottinghaus GE. Efficacy of a hydrated sodium calcium aluminosilicate to reduce the toxicity of aflatoxin and diacetoxyscirpenol. Poult Sci, 1993; 72(1): 51-59.
25. Murphy PA, Hendrich S, Landgren C, Bryant CM. Food mycotoxins: An update. J Food Sci, 2006; 71(5): 51-65.
26. Naseem MN, Saleemi MK, Abbas RZ, Khan A, Khatoon A, Gul ST, Imran M, Sindhu ZUD, Sultan A. Hematological and serum biochemical effects of aflatoxin B1 intoxication in broilers experimentally infected with fowl adenovirus-4 (FAdV-4). Pak Vet J, 2018; 38(2): 209-213.
27. Pestka JJ, Bondy GS. Alteration of immune function following dietary mycotoxin exposure. Can J Physiol Pharmacol, 1990; 68(7): 1009-1016.
28. Pier AC, McLoughlin ME. Mycotoxin suppression of immunity. In: J. Lacey (Ed.) Trichothecenes and Other Mycotoxins, 1985; 507-519.
29. Prajapati A, Verma Y, Swamy M, Dubey A. Efficacy of herbal binder in aflatoxin b1 induced toxicity on haematological profile in wistar rats. Indian J Field Vet, 2013; 9(2): 16-19.
30. Reddy RV, Taylor MJ, Sharma RP. Studies of immune function of CD-1 mice exposed to aflatoxin B1. Toxicology, 1987; 43(2): 123-132.
31. Reeta KH, Mehla J, Pahuja M, Gupta YK. Pharmacokinetic and pharmacodynamic interactions of valproate, phenytoin, phenobarbitone and carbamazepine with curcumin in experimental models of epilepsy in rats. Pharmacol Biochem Behav, 2011; 99: 399-407.
32. Rustemeyer SM, Lamberson WR, Ledoux DR, Rottinghaus GE, Shaw DP, Cockrum RR, Kessler KL, Austin KJ, Cammack KM. Effects of dietary aflatoxin on the health and performance of growing barrows. J Anim Sci, 2010; 88(11): 3624-3630.
33. Sakhare PS, Harne SD, Kalorey DR. Effect of toxiroak polyherbal feed supplement during induced aflatoxicosis, ochratoxicosis and combined mycotoxicoses in broilers. Vet Arhiv, 2007; 77(2): 129-146.
34. Sharma RP. Immunotoxicity of mycotoxins. J Dairy Sci, 1993; 76(3): 892-897.
35. Sharma V, Sharma C, Paliwal R, Pracheta SS, Sharma S. Ameliorative effects of Curcuma longa and curcumin on aflatoxin B1 Induced serological and biochemical changes in kidney of male mice. Asian J Biochem Pharmaceut Res, 2011a; 2: 338-351.
36. Sharma V, Sharma C, Sharma S. Influence of curcuma longa and curcumin on blood profile in mice subjected to aflatoxin B1. IJPSR, 2011b; 2(7): 1740-1745.
37. Soni KB, Lahiri M, Chackradeo P, Bhide SV, Kuttan R. Protective effect of food additives on aflatoxin-induced mutagenicity and hepatocarcinogenicity. Cancer Lett, 1997; 115(2): 129-133.
38. Srinivas L, Shalini VK, Shylaja M. Turmerin: A water soluble antioxidant peptide from turmeric (Curcuma longa). Arch Biochem Biophys, 1992; 292(2): 617-623.
39. Swamy AV, Gulliaya S, Thippeswamy A, Koti BC, Manjula DV. Cardioprotective effect of curcumin against doxorubicininduced myocardial toxicity in albino rats. Indian J Pharmacol, 2012; 44(1): 73-77.
40. Tung HT, Cook FW, Wyatt RD, Hamilton PB. The anemia caused by aflatoxin. Poult Sci, 1975; 54(6): 1962-1969.
41. Vachharajani V, Wang SW, Mishra N, El Gazzar M, Yoza B, McCall C. Curcumin modulates leucocytes and platelets adhesion in murine sepsis. Microcirculation, 2010; 17(6): 407- 416.
42. Valchev I, Kanakov D, Hristov TS. Investigations on haematological parameters and bone marrow morphology in broiler chickens with experimental aflatoxicosis. Agri Sci Technol, 2014; 6: 417-22.
43. Weeraphan C, Srisomsap C, Chokchaichamnankit D, Subhasitanont P, Hatairaktham S, Charoensakdi R, Panichkul N, Siritanaratkul N, Fucharoen S, Svasti J, Kalpravidh RW. Role of curcuminoids in ameliorating oxidative modification in β-thalassemia/Hb E plasma proteome. J Nutr Biochem, 2013; 24(3): 578-585.
44. Yuan G, Dai S, Yin Z, Lu H, Jia R, Xu J, Song X, Li L, Shu Y, Zhao X. Toxicological assessment of combined lead and cadmium: acute and sub-chronic toxicity study in rats. Food Chem Toxicol, 2014; 65: 260-268.