Tamer SEYHAN, Murat DENİZ, İ.Barış ÇAĞLAR, Hüseyin BORMAN

LABORATUVAR RATLARINDA MİKROANJİYOGRAFİ TEKNİĞİ

Ratlardaki mikrovasküler anatomiyi ortaya koymak için yapılan mikroanjiyografi çekim işlemi deneyim gerektirir ve genelde araştırmacılar bu konuda zorluk çekerler. Bu makalede rat epigastrik flebi mikroanjiyografisi için karotis arter kanülasyonu ile yapılan mikroanjiyografi tekniğimiz ayrıntılı olarak anlatılmış özellikle bu konuda tecrübesiz araştırmacılara yol gösterilmesi amaçlanmıştır. Aynı teknik kullanılarak çekim yapılacak bölgeye yakın ana damarlar kanüle edilip istenilen bölgenin mikroanjiyografisi çekilebilir.

Anahtar Kelimeler:

mikroanjiyografi, epigastrik flep, rat, mikrovasküler

MICROANGIOGRAPHY TECHNIQUE IN LABORATORY RATS

Microangiography which reveals the microvascular anatomy of the laboratory rats is a demanding procedure especially for inexperienced investigators. We explain our microangiography technique that is performed via carotis artery to reveal the microvascular anatomy of the rat epigastric flap in details and we intend to show the way to the inexperienced investigators on this subject. The technique may be used for any region by the canulation of main vicinity arteries by using the same injection technique.

Keywords:

Microangiography, epigastric flap, rat, microvascular,

PDF

___

Salmon M. Arteres de la Peau. Paris: Mason et cie, 1936. Edited by Taylor GI, Tempest MN. London: Churchill Livingstone, 1988.
Rees MJ, Taylor GI. A simplified lead oxide cadaver injection technique. Plast Reconstr Surg 1986;77(1):141-145.
Erol OO, Spira M, Levy B. Microangiography: a detailed technique of perfusion. J Surg Res 1980 ; 29(5):406-13.
Bayramiçli M. Laboratuar sıçanının anatomisi. Ameliyat. Bayramiçli M. Deneysel mikrocerrahi. Temel Araştırma, Doku ve Organ Nakil Modelleri Argos iletişim Hizmetleri 2005;119-184,75-98.
Van Rensburg C, Thandar AS, Moodley IG. Practical animal anatomy Butterworth Publishers Inc. Woburn, MA, 1980.
Hebel R, Strömberg MW.: Anatomy of the laboratory Rat Williams & Wilkins Co. Baltimore, 1976.
Grene EC: Anatomy of the Rat. New York, Hafner Publishing Company,1963.
Bergeron L, Tang M, Morris SF. A review of vascular injection techniques for the study of perforator flaps. Plast Reconstr Surg 2006;117(6):2050-2057.
Karamürsel S, Gündeşlioğlu Ö, Çelebioğlu S.: A new microopaque silicone mixture for cadaveric injection. Plast Reconstr Surg 2001; 108(6):1839-40.
0. Maehara N. Experimental microcomputed tomography study of the 3D microangioarchitecture of tumors. Eur Radiol 13:1559-1565,2003. WHO Inorganic Health Criteria, EHC 165: Inorganic lead. Geneva: World Health Organization, 1995.
1. Tang M, Geddes CR, Yang D, et al. Modified lead oxide-gelatin injection technique for vascular studies. J Clin Anat 2002;1:73-78.
2. Quinodoz P, Quinodoz M, Nussbaum JL, et al. Barium sulphate and soft-tissue radiology: Allying the old and the new for the investigation of animal cutaneous circulation. Br J Plast Surg 2002;55(8):664-667.
3. Özek C, Zhang F, Lineaweaver WC, Chin BT, Newlin L et al. New simplified radio-opaque injection technique for visualization of rat arteries. Microsurgery 1996;17(6):321-23.
4. Lerman A, Ritman EL. Evaluation of microvascular anatomy by micro-CT. Herz 1999; 24(7):531-533.
5. Wan SY, Kiraly AP, Ritman EL, et al. Extraction of the hepatic vasculature in rat using 3-D micro-CT images. IEEE Trans Med Imaging 2000;19(9):964-971.
6. Ito K, Tanaka E, Mori H, Nakazawa H, Tanino R. A microangiographic technique using synchrotron radiation to visualize dermal circulation in vivo. Plast Reconstr Surg 1998; 102(4): 1128-33.
7. Kormano M. An experimental technique of in vivo high resolution microangiography. Br J Radiol 1970;43(507):180-184.
8. Tang M, Yang D, Geddes CR, Morris SF. Anatomic Techniques. In P.N. Blondeel (Eds) Perforator Flaps anatomy, Technique & Clinical Applications. QMP Vol 1 2006;54-67.