Günnur KOÇER, Seher NASIRCILAR, Filiz BASRALI, Oktay KURU, Ümit Kemal ŞENTÜRK

Karbon Monoksit, Spontan Hipertansif Sıçanların Renal Direnç Arterlerinde Vasküler Tonusun Düzenlenmesine Katkıda Bulunur

Amaç: Vasküler tonusun düzenlenmesinde yer alan karbon monoksit CO , hipertansiyon gelişimi sırasında kompansatuar etki gösterebilir. Bu çalışma, spontan hipertansif sıçanlarda SHR HO / CO sisteminin renal direnç arterlerinin vasküler tonusu üzerine etkilerini değerlendirmeyi amaçlamıştır.Yöntem: Renal direnç arterlerinde endojen CO’nun vasküler tonusa katkısı, bir HO inhibitörü varlığında ve yokluğunda fenilefrin Phe kontraksiyon yanıtı ile test edildi. Ekzojen CO gevşeme yanıtı da CO donörü Carbon monoxide-releasing molecule CORM kullanılarak değerlendirildi. CO gevşeme yanıtının mekanizması, guanilat siklaz inhibitörü ODQ veya potasyum kanal blokörü TEA ile değerlendirildi. Renal direnç arterlerindeki HO-1 ve HO-2 protein ekspresyonları Western blot analizi ile saptandı. Bulgular: SHR’lerde kontrol grubuna kıyasla Phe’ye verilen kasılma yanıtı anlamı olarak yüksekti p

Anahtar Kelimeler:

CO, HO-1, SHR, renal direnç arteri

Carbon Monoxide Contributes to The Regulation of Vascular Tonus in Renal Resistance Arteries in Spontaneously Hypertensive Rats

Objective: Carbon monoxide CO , an end product of heme oxygenase HO involved in the regulation of vascular tone, may show a compensatory effect in the course of hypertension. This study aimed to assess the effects of the HO/CO system on the vascular tone of renal resistance arteries in spontaneously hypertensive rats SHRs .Methods: The contribution of endogenous CO to vascular tone in renal resistance arteries was evaluated by the phenylephrine Phe contraction response with or without an HO inhibitor. The effect of the exogenous CO relaxation response was assessed by a CO releasing molecule CORM . The mechanism of the CO relaxation effect was evaluated by a guanylate cyclase inhibitor, ODQ, or a potassium channel blocker, TEA. HO-1 and HO-2 enzyme protein expressions in renal resistance arteries were determined by Western blot analysis.Results: Phe-induced constriction responses were higher in renal resistance arteries of SHRs compared to control animals. The extent of the same type of constriction was even higher in the SHR group after inhibition of CO production. Relaxation responses to a CO donor, CORM, were greater in the SHR group versus the control group. TEA, but not ODQ, suppressed CORM olarak baskıladı p

Keywords:

CO, HO-1, SHR, renal resistance artery,

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