Mustafa OĞURLU, Selda ŞEN, Serdar ŞEN, Aslıhan KARUL

The antioxidative and antiinflammatory effects of hypoxemic resuscitation with HES 130/0.4 and modified gelatin solution on acute hemorrhagic shock in rabbits

Amaç: Bu çalışmada tavşanlarda hipoksemik koşullarda gerçekleştirilen akut hemorajik şok modelinde HES 130/0.4 veya modifiye jelatin solüsyonu gibi kolloid solüsyonlarının antioksidatif ve antiinflamatuvar etkileri araştırıldı.Çalışma planı: Yirmi bir adet Wistar albino cinsi Yeni Zelanda tavşanı rastlantısal olarak üç gruba ayrıldı; HES 130/0.4 solüsyonu (n=7 Voluven®, HES grubu), Jelatin solüsyonu (n=7 Gelafusine®, GEL grubu) ve kontrol grubu (n=7 %0.9 sodyum klorür). Hemorajik şok modeli, hipoksemik koşullar altında sol karotis arterinden 30 dakikada tahmin edilen kan hacminin %40’ını çekecek şekilde uygulandı. Hipovolemi dönemi 10 dakika süresince sağlandı. Hipovelemik dönem sonunda çekilen kan hacmi kan resüsitasyon sıvısı sodyum klorür hariç (sodyum klorür solüsyonu 1 birim hacim kaybı için 3 birim olacak şekilde) jugüler ven yoluyla 30 dakika süresince verildi. Tavşanlar hemoraji ve sıvı resüsitasyonu sırasında trakeostomi ile oda havasında spontan solunum yapmaktaydı (hipoksemik resusitasyon). Malondialdehit (MDA), glutatyon (GSH), interlökin (IL)-6, IL-10, tümör nekroz faktör alfa (TNF)-a konsantrasyonları hemorajik şok öncesi ve sıvı resüsitasyonu sonrasında tüm kan örneklerinde ölçüldü.Bulgular: Kontrol grubunda diğer gruplara göre MDA, TNF-a, IL-6 değerleri en yüksek, buna karşılık GSH ve IL-10 değerleri en düşüktü. Grup HES’de grup GEL’e göre MDA, TNF-a ve IL-6 değerleri daha düşük fakat IL-10 ve GSH düzeyleri ise daha yüksekti (p

Akut hemorajik şok oluşturulan tavşanlarda HES 130/0.4 ve modifiye jelatin solüsyonları ile yapılan hipoksemik resüsitasyonun antioksidatif ve antiinflamatuvar etkileri

Background: The aim of this study was to investigate antioxidative effects and antiinflammatory properties of colloid fluids such as HES 130/0.4 or modified gelatin solution in rabbits during resuscitation of acute hemorrhagic shock under hypoxemic conditions.Methods: Twenty-one New Zealand Wistar albino rabbits were randomly allocated to one of the three experimental groups: HES 130/0.4 solution (n=7, Voluven®, group HES), gelatine solution (n=7, Gelafusine®, group GEL) and control (n=7, sodium chloride 0.9%, Saline group). Hemorrhagic shock was induced by controlled blood withdrawal (40% of total estimated blood volume) from the left carotid artery over 30 minute under hypoxemic conditions. The period of hypovolemia was maintained for 10 minute All resuscitation solutions, except saline solution (3 volumes of saline/1 volume of blood loss for saline), were infused as withdrawn blood volume via the jugular vein over 30 minute after the hypovolemic period under hypoxemic conditions. Rabbits were breathing spontaneously via tracheotomy in room air during hemorrhage and fluid resuscitation periods (hypoxemic resuscitation). Malondialdehyde (MDA), glutathione (GSH), interleukin (IL)-6, IL-10 and tumor necrosis factor alpha (TNF)-a concentrations were measured before hemorrhagic shock and after fluid resuscitation in all blood samples.Results: The values of the MDA, TNF-a, IL-6 levels were the highest, whereas IL-10 and GSH levels were the lowest in the Saline group compared to the other groups. The MDA, TNF-a, IL-6 levels were lower, but the IL-10 and GSH levels were higher in group HES compared to group GEL (p

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