Makbule ERGİN, Ali YEĞİNSU, Kürşat GÜRLEK

Effects on inflammatory cytokines and oxidative stress markers of pneumoperitoneum performed after thoracotomy in rats

Amaç: Bu çalışmada pnömoperiton işleminin torakotomi sonrası inflamasyon üzerine etkileri araştırıldı. Çalışma planı: Otuz iki adet 280-320 g ağırlığında Wistar cinsi erkek sıçan her birinde sekiz sıçan olan dört gruba ayrıldı. Kontrol grubunda sıçanlara sadece anestezi uygulandı. Torakotomi grubunda sol torakotomi sonrası 30 dakika periyodlu otomatik retraktör yerleştirildi. Pnömoperiton grubunda batın içerisine 5 ml oda havası enjekte edildi. Torakotomi + pnömoperiton grubunda ise torakotomi uygulandı ve batın içerisine 5 ml oda havası enjekte edildi. Yirmi dört saat sonra tüm gruplardan kan ve akciğer örnekleri alındı ve -80 °C’de saklandı. Bulgular: Torakotomi grubunda tümör nekroz faktörüalpha, interlökin-6 ve protein karbonil düzeyleri kontrol grubundan anlamlı olarak daha yüksek idi fakat pnömoperiton ve torakotomi + pnömoperiton grupları arasında fark yok idi. Sonuç: Torakotomi sonrasında yapılan pnömoperiton işlemi torakotominin neden olduğu inflamatuvar reaksiyonu ve oksidatif stresi artırıcı bir etki yapmamaktadır. Pnömoperiton efektif ve kolay uygulanan bir yöntemdir.

Sıçanlarda torakotomi sonrası yapılan pnömoperiton işleminin inflamatuvar sitokinler ve oksidatif stres belirteçleri üzerindeki etkisi

Background: This study aims to investigate the effects of pneumoperitoneum on inflammation after thoracotomy. Methods: Thirty-two male Wistar type rats weighing 280-320 g were divided into four groups of eight rats each. In the control group rats were only anaesthetized. In thoracotomy group an automatic retractor was inserted for a 30-minute period after left thoracotomy. In the pneumoperitoneum group, 5 ml of room air was injected intraperitoneally. In the thoracotomy + pneumoperitoneum group, thoracotomy was performed and 5 ml of room air was injected intraperitoneally. Blood and lung tissue samples were taken at the $24^{th}$ hour in all groups and were stored at -80 °C. Results: In the thorocotomy group, the tumor necrosis factor-alfa, interleukin-6 and protein carbonyl levels were significantly higher than in the control group but no difference was found between the pneumoperitoneum and the thoracotomy + pneumoperitoneum groups. Conclusion: Pneumoperitoneum performed after thoracotomy does not increase the inflammatory reaction and oxidative stress caused by thoracotomy. Pneumoperitoneum is an effective and easily performed method.

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