Effects of induced pressure and clamping force by vascular clamps on the vascular endothelium of rat aorta
Amaç: Bu çalışmada sıçan aortu vasküler endotelinde vasküler klemplerin etkileri araştırıldı.Ça lış ma pla nı: Çalışmaya 32 erkek SpragueDawley sıçan (ağırlık 242±26 g; yaş 9-11 hafta) dahil edildi. Sıçanlar dört eşit gruba ayrıldı: Kontrol grubu: Abdominal aorta klemp uygulanmadı; grup 1: Abdominal aorta 10 dakika plastik bulldog klemp uygulandı; grup 2: Abdominal aorta 10 dakika metal bulldog klemp uygulandı; grup 3: Abdominal aorta 10 dakika mikrovasküler klemp uygulandı. On dakikanın sonunda tüm gruplarda vasküler klemple oklüde edilen segmentler çıkarıldı ve endotel yapılar histopatolojik olarak değerlendirildi.Bul gu lar: Kontrol grubunda normal hücresel dizilim ve yapı tespit edildi. En ciddi endotel yüzey yaralanması grup 3'te, orta düzeyde endotel yaralanması grup 2'de, en hafif endotel yaralanması ise grup 1'de gözlendi. Histopatolojik olarak vasküler endotelyal büyüme faktörü ekspresyonunun grup 1 ve 2'de (sırasıyla 2.8±0.5 ve 3.3±0.5) kontrol örneklere göre (2.0±0.5) artmış olduğu saptandı.So nuç: Vasküler klempler, oluşturduğu basınç ve sıkma kuvvetine bağlı olarak endotel yaralanmasına neden olabilir.
Sıçan aortu vasküler endotelinde vasküler klempler tarafından oluşturulan basınç ve sıkma kuvveti etkileri
Background: This study aims to investigate the effects of vascular clamps on the vascular endothelium of rat aorta.Methods: The study included 32 male Sprague-Dawley rats (weight 242±26 g; age 9 to 11 weeks). Rats were divided into four equal groups: control group: no clamp was applied on abdominal aorta; group 1: plastic bulldog clamp was applied on abdominal aorta for 10 minutes; group 2: metal bulldog clamp was applied on abdominal aorta for 10 minutes; group 3: microvascular clamp was applied on abdominal aorta for 10 minutes. At the end of 10 minutes, segments occluded with vascular clamp were excised in all groups and endothelial structures were evaluated histopathologically.Results: Normal cellular sequencing and structure were determined in control group. Most severe injury of the endothelial surface was observed in group 3, moderate level endothelial injury was observed in group 2, while mildest endothelial injury was observed in group 1. Increased vascular endothelial growth factor expression levels were detected histopathologically in groups 1 and 2 (2.8±0.5 and 3.3±0.5, respectively) when compared with the control subjects (2.0±0.5). Conclusion: Due to their induced pressure and clamping force, vascular clamps may cause endothelial injury.
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