Serkan KAYA, Sinem ALBAYRAK KAYA, Elif POLAT, Zeynep FİDANOL GÜRBOĞA, Hamza Malik OKUYAN, İhsan KARABOĞA, Yasin DURAN, Fatin Rüştü POLAT

Bir sıçan modelinde lipopolisakkarit uyarımlı akut akciğer hasarı üzerine hesperetinin koruyucu etkileri

Amaç: Bu deneysel çalışmada, doğal bir flavonoid olan hesperetinin sıçanlarda lipopolisakkarit uyarımlı akut akciğer hasar modeli üzerindeki etkileri incelendi. Çalışma planı: Mart 2019 - Mayıs 2019 tarihleri arasında ağırlıkları yaklaşık 250-300 g olan toplam 18 erişkin erkek Wistar albino sıçan, kontrol, lipopolisakkarit ve lipopolisakkarit + hesperetin grubu olmak üzere rastgele üç gruba ayrıldı (her grupta n=6). Akciğer dokusunda ıslak/kuru ağırlık oranı belirlendi. Histopatolojik değişiklikler ışık ve elektron mikroskobu ile incelendi. Pulmoner nükleer faktör-kappa beta, indüklenebilir nitrik oksit sentaz ve alfa-düz kas antijeni indirekt immünohistokimyasal yöntem ile belirlendi. Pulmoner apoptoz, terminal deoksinükleotidil transferaz dUTP çentik uç işaretleme yöntemi ile belirlendi. Tümör nekroz faktör-alfa, interlökin-1 beta, interlökin-6 ve interlökin-10 konsantrasyonları enzim bağlı immunosorbent testi ile belirlendi. Bulgular: Hesperetin tedavisi akciğer doku mimarisini anlamlı düzeyde iyileştirdi ve ıslak/kuru ağırlık oranı, nükleer faktör-kappa beta, indüklenebilir nitrik oksit sentaz ve alfa-düz kas antijeni ekspresyonu, pulmoner apoptoz ve proinflamatuvar sitokin düzeylerini azalttı. Sonuç: Çalışma sonuçlarımız, hesperetinin sıçanlarda proinflamatuvar sitokin kaskadı, nükleer faktör-kappa beta sinyal yolağı aktivasyonu ve apoptozun baskılanması ile lipopolisakkarit uyarımlı akut akciğer hasarına karşı güçlü bir koruyucu etkiye sahip olduğunu göstermektedir

Protective effects of hesperetin on lipopolysaccharide-induced acute lung injury in a rat model

Background: In this experimental study, we aimed to investigate theeffects of hesperetin, a natural flavonoid, on a lipopolysaccharideinduced acute lung injury model in rats.Methods: Between March 2019 and May 2019, a total of 18 adultmale Wistar albino rats, weighing approximately 250 to 300 g, wererandomly divided into three groups as control, lipopolysaccharide,and lipopolysaccharide + hesperetin groups (n=6 in each group). Thewet/dry weight ratio of lung tissue was determined. Histopathologicalchanges were examined using light and scanning electron microscopy.Pulmonary nuclear factor-kappa beta, inducible nitric oxide synthase,and alpha-smooth muscle antigen activity were determined with indirectimmunohistochemical methods. Pulmonary apoptosis was detectedwith the terminal deoxynucleotidyl transferase dUTP nick-end labelingmethod. Tumor necrosis factor-alpha, interleukin-1 beta, interleukin-6,and interleukin-10 concentrations were measured with enzyme-linkedimmunosorbent assay.Results: Treatment with hesperetin significantly improved thearchitecture of lung tissue and reduced the wet/dry weight ratio,nuclear factor-kappa beta, inducible nitric oxide synthase, and alphasmooth muscle antigen expression, pulmonary apoptosis, and levels ofproinflammatory cytokines.Conclusion: Our study results suggest that hesperetin has a potentprotective effect against lipopolysaccharide-induced acute lung injury inrats via suppression of the proinflammatory cytokine cascade, nuclearfactor-kappa beta, signaling pathway activation, and apoptosis.

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