Akut alt ekstremite iskemi-reperfüzyon hasarında edaravonun renal koruyucu etkisi

Amaç: Bu deneysel çalışmada, sıçan modelinde akut alt ekstremite iskemi/reperfüzyonuna bağlı böbrek hasarında edaravonun etkinliği araştırıldı. Çalışma planı: Bu çalışmada Haziran 2015 - Ağustos 2015 tarihleri arasında toplam 40 erkek Wistar sıçan kullanıldı. Sıçanlar sham, iskemi/reperfüzyon, edaravon ve solvent gruplarına randomize edildi (n=10 her bir grupta). İnfrarenal abdominal aort 120 dk. süreyle klemlendi ve sonrasında klemp kaldırılarak 120 dk. reperfüze edildi. İskemi indüksiyonundan 30 dk. önce edaravon intravenöz olarak verildi. Serum ve böbrek doku örnekleri biyokimyasal ve histopatolojik olarak analiz edildi. Bulgular: Edaravon serum ve doku malondialdehit düzeylerini iskemi/reperfüzyon grubunda azalttı. Serum süperoksit dismutaz aktivitesi, iskemi/reperfüzyon ve solvent gruplarına kıyasla, edaravon grubunda anlamlı düzeyde yüksekti. Serum nitrik oksit düzeyi iskemi/reperfüzyon grubunda, sham grubuna kıyasla, rakamsal olarak yüksek bulundu. Edaravon serum nitrik oksit düzeyini düşürdü. Serum nitrik oksit düzeyi edaravon grubunda, solvent grubundan, daha düşük idi. Doku nitrik oksit düzeyi iskemi/reperfüzyon grubunda, sham grubundan, anlamlı düzeyde daha yüksek idi. İskemi/ reperfüzyon grubunda histopatolojik değişikliklerde edaravon ile düzelme görüldü. Sonuç: Edaravon alt ekstremite iskemi/reperfüzyonuna bağlı renal hasarı azalttı. Bu nedenle, aort ve periferik damar cerrahisinde akut iskemi/reperfüzyon hasarını hafifletmede kullanılabilir.

Renoprotective effect of edaravone in acute limb ischemia/reperfusion injury

Background: In this experimental study, we aimed to investigatethe efficacy of edaravone on renal injury due to acute lower limbischemia/reperfusion in a rat model.Methods: Between June 2015 and August 2015, a total of40 male Wistar rats were used in this study. The rats wererandomly divided into the sham, ischemia/reperfusion,edaravone, and solvent groups (n=10 in each). The infrarenalabdominal aorta was clamped for 120 min and was, then,reperfused for 120 min after clamp removal. Edaravone wasadministered intravenously 30 min before the induction ofischemia. Serum and kidney tissue samples were subjected tobiochemical and histopathological analyses.Results: Edaravone decreased the serum and tissuemalondialdehyde levels in the ischemia/reperfusion group. Theserum superoxide dismutase activity in the edaravone group wassignificantly higher than the ischemia/reperfusion and solventgroups. The serum nitric oxide level in the ischemia/reperfusiongroup was numerically higher than the sham group. The serumnitric oxide level was decreased by edaravone. The serum nitricoxide level was lower in the edaravone group than the solventgroup. The tissue nitric oxide level was significantly higher inthe ischemia/reperfusion than the sham group. In the ischemia/reperfusion group, the histopathological changes were improvedby edaravone.Conclusion: Edaravone ameliorated renal injury caused bylower-limb ischemia/reperfusion. Therefore, it can be used toameliorate acute ischemia/reperfusion injury during aortic andperipheral vascular surgery

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