İbrahim METEOĞLU, Filiz ALKAYA, Aytuğ KOÇYİĞİT, Hüseyin OKUTAN, Tunay KURTOĞLU, Şenol GÜLMEN, Duygu KUMBUL DOĞUÇ

Aortik iskemi reperfüzyon modelinde ozon tedavisinin uzak organ miyokardiyal hasar üzerine etkisi

Amaç: Bu çalışmada sıçanlarda infrarenal abdominal aortun oklüzyonu-reperfüzyonu sonrası miyokardiyal iskemi reperfüzyon hasarına ozonun etkisi araştırıldı. Çalışma planı: Otuz iki Wistar albino sıçan (200-250 g ağırlığında) dört eşit gruba randomize edildi. Kontrol (sham) grubunda laparotomi ve oklüzyon olmaksızın infrarenal abdominal aort diseksiyonu uygulandı. Kontrol+ozon grubunda 10 gün 1 mg/kg/gün intraperitoneal ozon uygulandı. Daha sonra, kontrol+ozon grubunda laparotomi ve oklüzyon olmaksızın infrarenal abdominal aort diseksiyonu uygulandı. Aortik iskemi reperfüzyon ve aortik iskemi reperfüzyon+ozon gruplarında infrarenal abdominal aort diseksiyonu uygulandı, takiben infrarenal abdominal aorta 60 dakika kros-klemp konularak ve 60 dakika kros-klemp kaldırılarak sırasıyla iskemi ve reperfüzyon gerçekleştirildi. Miyokardiyal örneklerde dokulardaki malondialdehit düzeyleri ve süperoksit dismutaz, katalaz ve myeloperoksidaz aktivite düzeyleri ölçüldü. Plazma tümör nekroz faktörü, interlökin-6 ve troponin-I düzeyleri ölçüldü. Miyokardiyal örneklerin histopatolojik incelemesi yapıldı. Bulgular: Biyokimyasal analiz; ozonun doku süperoksit dismutaz ve katalaz düzeylerini ve plazma troponin -I düzeyini anlamlı olarak azaltırken (aortik iskemi reperfüzyona karşı p

The effect of ozone treatment on remote organ myocardial injury in an aortic ischemia-reperfusion model

Background: This study aims to investigate the effect of ozone on myocardial ischemia-reperfusion injury occurring after occlusion - reperfusion of infrarenal abdominal aorta in rats.Methods: Thirty-two Wistar albino rats (weighing 200-250 g) were randomized into four equal groups. The control (sham) group underwent laparotomy and dissection of the infrarenal abdominal aorta without occlusion. Intraperitoneal ozone was applied for 10 days 1 mg/kg/day in the control+ozone group. Afterwards, control+ozone group underwent laparotomy and dissection of the infrarenal abdominal aorta without occlusion. Aortic ischemia-reperfusion and aortic ischemia -reperfusion+ozone groups underwent dissection of the infrarenal abdominal aorta, followed by achieving ischemia and reperfusion by cross-clamping the infrarenal abdominal aorta for 60 minutes and removing the cross-clamp for 60 minutes, respectively. The tissue levels of malondialdehyde and activity levels of superoxide dismutase, catalase, and myeloperoxidase were measured in the myocardial specimens. The tumor necrosis factor, interleukin-6 and troponin-I levels were measured in the plasma. A histopathological examination of the myocardial specimens was undertaken.Results: Biochemical analysis showed that aortic ischemia-reperfusion significantly increased (p

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