İlker AKAR, Oktay BURMA, Ayhan UYSAL, Kadir Kaan ÖZSİN, Bilal ÜSTÜNDAĞ, Hanifi İbrahim ÖZERCAN, Ali RAHMAN

Alt ekstremite iskemi reperfüzyonunun yol açtığı akciğer hasarında melatoninin koruyucu etkinliği

Amaç: Alt ekstremite iskemi sonrası reperfüzyon döneminde nötrofil infiltrasyonuna ve serbest oksijen radikallerine bağlı olarak akciğer hasarı oluşmaktadır. Başlıca pineal bezden salgılanan melatonin, çeşitli fizyolojik ve nöroendokrin fonksiyonların düzenlenmesinde rol oynar. Bu çalışmada, deneysel alt ekstremite iskemi-reperfüzyonuna (İ-R) bağlı oluşan akciğer hasarı ve bunları önlemede melatoninin koruyucu etkisi araştırıldı.Çalışma planı: Çalışmada, 40 adet Yeni Zelanda cinsi beyaz erkek tavşan kullanıldı. Tavşanlar rasgele dört gruba ayrıldı: Grup 1 (sham grubu, n=10), grup 2 (I-R grubu, n=10), grup 3 (preiskemik tedavi grubu, n=10) ve grup 4 (prereperfüzyon tedavi grubu, n=10). Grup 1'de sadece anestezi uygulandı ve 6 saat bekleme sonrası kan ve doku örnekleri alındı. Grup 2, 3 ve 4'de anestezi verildikten sonra iki taraflı arka ekstremite, kalça eklemi hizasından turnike ile sıkıldı. Dört saat iskemi sağlandıktan sonra turnike açıldı ve iki saat reperfüzyon sonrası kan-doku örnekleri alındı. Melatonin, grup 3'te iskemiden ve grup 4'te reperfüzyondan 15 dakika önce 10 mg/kg intraperitoneal yolla verildi. Alınan kan örneklerinde plazma malondialdehit (MDA), akciğer dokusunda ise doku MDA düzeyleri saptandı. Ayrıca akciğerler histopatolojik olarak incelendi.Bulgular: Doku ve plazma MDA seviyeleri grup 4'te grup 2 ve grup 3'e göre anlamlı derecede daha düşük bulundu (p

Protective effect of melatonin on lung injury caused by ischemia-reperfusion of the lower extremities

Background: Lung injury occurs following lower limb reperfusion due to neutrophil infiltration and free oxygen radicals. Melatonin, the chief secretory product of the pineal gland, plays a role in the regulation of various physiological and neuroendocrine functions. In this study, injury in the lungs due to experimental lower extremity ischemia-reperfusion (I-R) and the protective effect of melatonin were investigated.Methods: Forty male New Zealand white rabbits were used in the study. Rabbits were randomly divided into four groups: Group 1 (sham group, n=10), group 2 (I-R group without treatment, n=10), group 3 (preischemia treatment group, n=10), group 4 (prereperfusion treatment group, n=10). Only anesthesia was performed to rabbits in group 1 and after 6 hours blood and tissue samples were taken. In groups 2, 3 and 4, after anesthesia tourniquets were applied to bilateral hind limbs at the hip level. After 4 hours of ischemia, the tourniquets were removed and following two hours of reperfusion period, blood and tissue samples were taken. Melatonin (10 mg/kg) was administered intraperitoneally 15 minutes before ischemia to group 3, and 15 minutes before reperfusion to group 4. Plasma malondialdehyde (MDA) levels were measured in blood samples and lung tissue MDA levels were determined. Additionally, lung tissue was examined histopathologically.Results: The levels of tissue and plasma MDA were found to be significantly lower in group 4 when compared to groups 2 and 3 (p

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