Türkiye’den Crocidura suaveolens, Crocidura leucodon, Sorex raddei (Mammalia: Eulipotyphla) Türlerinin Sitogenetik Özellikleri: Konstitütif Heterokromatin Dağılımı

Bu çalışmada Türkiye’nin üç faklı lokalitesinden elde edilen üç farklı türe ait ( Crocidura suaveolens, Crocidura leucodon, Sorex raddei) 5 küçük memeli hayvanın standart karyotipi ve C band yöntemi kullanılarak kromozomlardaki heterokromatin dağılımı araştırılmıştır. Crocidura suaveolens karyotipi 2n=40, temel kromozom kol sayısı (NFa)= 46, X=submetasentrik (SM), Y=akrosentrik (A); Sorex raddei karyotipi 2n=36, NFa=66, X=A, Y=A; Crocidura leucodon karyotipi 2n=28, NFa= 46, X=SM, Y=A şeklindedir. C. suaveolens, C.leucodon, S. raddei karyotiplerinde C-bant heterokromatin dağılımı Türkiye populasyonlarında ilk defa bu çalışma ile tanımlanmıştır.

Cytogenetic Characteristics of Crocidura suaveolens, Crocidura leucodon, Sorex raddei (Mammalia: Eulipotyphla) from Turkey: Constitutive Heterochromatin Distribution

In this study, heterochromatin distribution in the chromosomes of five small mammals of three different species (Crocidura suaveolens, C. leucodon, Sorex raddei) from four different localities of Turkey were inestigated by using standard karyotype and C-banding method. Crocidura suaveolens karyotype included 2n=40 chromosomes, fundamental number of autosomal arms (NFa)= 46, X=submetacentric (SM), Y=acrocentric (A); Sorex raddei karyotype was 2n=36, NFa=66, X=A, Y=A; Crocidura leucodon karyotype was 2n=28, NFa= 46, X=SM, Y=A. C-banding heterochromatin distribution in C. suaveolens, C.leucodon, S. raddei karyotypes was reported in this study for the first time in Turkey populations. 

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  • [1] Dubey S, Zaitsev M, Cosson JF, Abdukadier A, Vogel P. Pliocene and Pleistocene diversification and multiple refugia in a Eurasian shrew (Crocidura suaveolens group). Mol Phylogenet Evol., 38(3):635-47, 2006.
  • [2] Krystufek B, Vohralík V. Mammals of Turkey and Cyprus, Introduction, Checklist, Insectivora. Koper, Slovenia: Knjižnica Annales Majora; 2001.[3] Maddalena T, Ruedi M. Chromosomal evolution in the genus Crocidura (Insectivora: Soricidae). In: Merritt, J.F., Kirkland, G.L.Jr. & Rose, R.K. (eds): Advances in the biology of Shrews. Carnegie Museum Natural History Special Publication, 18: 335-344, 1994.[4] Zima J, Lukáčová L, Macholán M. Chromosomal evolution in shrews. In: Wójcik J.M. & Wolsan M. (eds.), The evolution of shrews. Mammal Research Institute, Polish Academy of Sciences, Bialowieza, 175–218, 1998.[5] Biltueva L, Perelman PL, Polyakov VA, Zima J, Dannelid E, Borodin MP, Graphodatsky SA. Comparative chromosome analysis in three Sorex species S. raddei, S. minutus and S. caecutiens. Acta Theriol., 45(1): 119-130, 2000.[6] Kefelioğlu H, Tez C. The Distribution problem of Crocidura russula (Hermann, 1780) (Mammalia: Insectivora) in Turkey. Turk J Zool., 23: 247 – 251, 1999.[7] Tez C. Taxonomy and Distribution of the White-Toothed Shrews (Crocidura) (Soricidae: Insectivora: Mammalia) of Turkey. Turk J Zool., 24: 365-374, 2000.[8] Arslan A, Zima J. Karyotypes of the mammals of Turkey and neighbouring regions: review. Folia Zool., 63: 1-62, 2014.[9] Wojcik MJ, Ratkiewicz M, Searle B. Evolution of the common shrew Sorex araneus: chromosomal and molecular aspect. Acta Theriol., 47(1): 139-167, 2002.[10] Ford CE, Hamerton JL. A colchicine, hypotonic citrate, squash sequence for mammalian chromosomes. Stain Technol., 31: 247-251, 1956.[11] Summer AT. A simple technique for demonstrating centromeric heterochromatin. Exp Cell Res., 75: 304-306, 1972.[12] Dubey S, Salamin N, Ruedi M, Barrière P, Colyn M, Vogel P. Biogeographic origin and radiation of the Old World crocidurine shrews (Mammalia: Soricidae) inferred from mitochondrial and nuclear genes. Mol Phylogenet Evol., 48: 953–963, 2008.[13] Catzeflis F, Maddalena T, Hellwing S, Vogel P. Unexpected findings on the taxonomic status of East Mediterranean Crocidura russula auct. (Mammalia, Insectivora). Mamm Biol., 50: 185–201, 1985.[14] Meylan A, Hausser J. Position cytotaxonomique de quelques musaraignes du genre Crocidura au Tessin Mammalia, Insectivora). Rev. suisse zool., 81: 701-710, 1974.
  • [15] Zima, J. & Král, B. Karyotypes of European mammals II. Acta Sc. Nat. Brno., 18(8): 1–62, 1984.[16] Tembotova FA. On the systematics of white-toothed shrews in the Caucasus. Mammalian fauna and ecology in the Caucasus Nalchik., 163–189 (in Russian), 1987.[17] Graphodatsky AS, Radjabli SI, Sharshov AV, Zaitsev MV. Karyotypes of five Crocidura species of the USSR fauna. Citologiya., 30: 1247–1252. (in Russian with English summary), 1988.[18] Ivanitskaya E. Constitutitive heterochromatin and nucleolar organizer regions in karyotypes of some shrews (Soricidae, Insectivora). Genetika., 25: 1188-1198 (in Russian), 1989.[19] Atanasov IN, Chassovnikarova GT. Karyotype Characteristics of Crocidura leucodon Herman, 1780 and Crocidura suaveolens Pallas, 1811 (Mammalia: Insectivora: Soricidae) in Bulgaria. Acta Zool Bulgar., 61(2):71-78, 2008.[20] Orlov VN, Radjabli SI, Malygin VM, Chotolchu N, Kovalskaja JuM, Bulatova NS, Baskevic ML. The karyotypes of the mammals of Mongolia: Geografiya i dinamika rastitelnogo i zivotnogo mira MNR, Nauka, Moskva, pp. 149-164 (in Russian), 1978.[21] Sokolov VE, Tembotov AK. Vertebrates of the Caucasus. Mammals. Insectivores. Nauka, Moskva, (in Russian), 1989.[22] O’Brien SJ, Menninger JC, Nash WG. Atlas of mammalian chromosomes. Wiley-Liss, Hoboken, NJ., 2006.[23] Biltueva L, Vorobieva N, Perelman P, Trifonov V, Volobouev V, Panov V, Ilyashenko V, Onischenko S, O’Brien P, Yang F, Ferguson-Smith M, Graphodatsky A. Karyotype Evolution of Eulipotyphla (Insectivora): The Genome Homology of Seven Sorex Species Revealed by Comparative Chromosome Painting and Banding Data. Cytogenet Genome Res., 135: 51–64, 2011.