Mustafa ŞİMŞEKYILMAZ, Taner ARABACI, Elif BİLİCİ, Beyza Nur ŞAHİN

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Kronik Periodontitisli, Periodontal Sağlıklı; Sigara İçen/İçmeyen Bireylerde Doku Düzeyinde Vazoaktif İntestinal Peptit(VIP) Seviyelerinin İmmünohistokimyasal Olarak Belirlenmesi

Amaç: Periodontal durum ve sigara kullanımının vazoaktif intestinal peptit(VIP) seviyelerine etkisini incelemek. Gereç ve Yöntem: Kronik periodontitisli (KP) ve periodontal sağlıklı sigara içen ve içmeyen dört grup oluşturulmuştur. (Sigara kullanımı yok ve periodontal açıdan sağlıklı bireyler:S, Sigara kullanımı var ve periodontal açıdan sağlıklı bireyler:SS, Sigara kullanımı yok ve kronik periodontitisli bireyler:KP, Sigara kullanımı var ve kronik periodontitisli bireyler:KPS). Alınan periodontal dokular immünohistokimyasal olarak boyanmış ve VIP(+) hücrelerin steoroloji yöntemiyle ortalama sayısal yoğunlukları belirlenmiştir. Bulgular: Bütün gruplar arasında VIP reseptör ortalama sayısal yoğunluk seviyelerinde istatistiksel olarak anlamlı fark bulunmuştur (p<0.05). VIP’te ise S, SS, KP grupları arasında anlamlı fark gözlenirken (p<0.05); KP ve KPS grupları arasında anlamlı fark gözlenmemiştir (p>0.05). Sonuç: Pro-inflamatuar yolaktaki sitokinleri etkileyen VIP anti-inflamatuar bir nöropeptit olarak çalışmaktadır. Bu proteinde ve reseptöründe, KP ve sigara kullanımı ile periodontal dokulardaki seviyelerinde artış görülmektedir.
Anahtar Kelimeler:

vazoaktif intestinal peptit, kronik periodontitis, sigara kullanımı

Immunohistochemical Determination Of Vasoactive Intestinal Peptide(VIP) Levels In Smokers and Non-Smokers Chronic Periodontitis Patients And Periodontal Healthy Individuals

Background: The main aim of this study to investigate the effect of periodontal status and smoking on vasoactive intestinal peptide(VIP) levels on periodontal tissue. Material and Method: Patients were divided four groups. Groups were named non-smokers and periodontal healthy individuals(H), smokers and periodontal healthy individuals(HS), non-smokers and chronic periodontitis patients(CP), smokers and chronic periodontitis patients(CPS). Collected periodontal tissue were stained immunohistochemically and then the numerical densities of the VIP(+) cells were determined by steorology method. Results: VIP receptor numerical density levels were statistically significant between all groups (p <0.05). A significant difference was observed between H, HS and CP groups at the VIP level (p <0.05). No significant difference was observed between CP and CPS groups (p> 0.05). Conclusion: The VIP acts as an anti-inflammatory neuropeptide that affects the cytokines in the pro-inflammatory pathway. There is an increase at the level of this protein and its receptor in periodontal tissue when patients has periodontal disease or they are smokers.
Keywords:

vasoactive intestinal peptite, chronic periodontitis, smoking,

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Türk Diş Hekimliği Araştırma Dergisi-Cover
  • Başlangıç: 2022
  • Yayıncı: Ondokuz Mayıs Üniversitesi
Arşiv
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