Ergun KAYA, Selin GALATALI, Sevinç GÜLDAĞ, Bilge ÖZTÜRK, Muammer CEYLAN, Onur ÇELİK, İrem AKTAY

Biyoreaktör Sistemleri Kullanılarak Sıvı Besiyeri Ortamında Sekonder Metabolit Üretimi için Tıbbi Bitkilerin Kitlesel Çoğaltımı: SETISTM And RITA®

Mikroçoğlatım teknikleri, bitkilerin kitlesel çoğaltımı için etkin ve önemli bir yöntemdir. Bazı kültür çeşitleri hala doku kültürü yöntemleriyle çoğaltıma direnç gösterse de, somatik ve zigotik embriyogenez, organogenez, mikroçoğaltım gibi başarılı uygulamalar, farklı bitki türlerinin farklı çeşitlerinden rapor edilmiştir. Başarılı otomasyon, yüksek kitlesel üretim, kolay manupulasyon, kültür ortam bileşenlerinin basit kalibrasyonu gibi avantajlara sahip olması nedeniyle in vitro sıvı kültür sistemleri, yarı katı sistemlere göre çok daha etkin sistemlerdir. Sıvı besiyeri kullanımına dayalı periyodik daldırma biyoreaktör sistemleri (TIS), düşük üretim maliyeti ve yüksek çoğaltım oranı gibi faydalar sağlamaktadır. Bu derleme çalışması, SETISTM ve RITA® gibi, iki farklı periyodik daldırma biyoreaktör sistemlerinin kullanımına dayalı geliştirilen mikroçoğaltım yöntemlerinin avantajlarını tanımlamayı amaçlamaktadır.

Anahtar Kelimeler:

Mikroçoğaltım, Bitki ıslahı, Bitki klonal çoğaltımı, TIS

Mass Production of Medicinal Plants for Obtaining Secondary Metabolite Using Liquid Mediums Via Bioreactor Systems: SETISTM And RITA®

Micropropagation techniques provide effective and important ways for plant mass propagation. While some cultivated species are still accepted as comparatively resistant to tissue culture, there are a lot of successful applications of somatic and zygotic embriyogenesis, organaogenesis, micropropagation have been reported from different type of plant species. In vitro liquid culture systems are thought to be more efficient than semi-solid culture systems because of having great advantages of them such as successful automation, big mass production, easy handling, simple calibration of medium components and culture conditions. Temporary immersion bioreactor systems (TIS) based on liquid medium usage have been provided many utilities such as reduction of production cost and higher proliferation rate. This review aimed to describe advantages of micropropagation protocols developed using two different temporary immersion bioreactor systems, SETISTM and RITA®.

Keywords:

Micropropagation, plant breeding, plant clonal propagation, TIS,

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