ÇEŞİTLİ BİTKİ ÇAYLARININ MONOAMİN OKSİDAZ İNHİBİSYONLARININ VE ANTİOKSİDAN ETKİLERİNİN BELİRLENMESİ

Meyve çaylarının terapötik özellikleri içerdikleri polifenolik flavonoidlerden kaynaklanmaktadır. Bir flavoenzim olan monoamin oksidazı (EC 1.4.3.4, MAO) inhibe eden bileşiklerin, Alzheimer ve Parkinson gibi nörodejeneratif rahatsızlıkların tedavisinde ve çeşitli nörolojik rahatsızlıklarda ilaç olarak kullanıldığı bilinmektedir. Antioksidan özelliğe sahip doğal bileşiklerin nörodejenerasyonda önemli bir rol oynayan oksidatif stresi azaltarak bu hastalıkları önlediğine dair çok sayıda çalışma vardır. Bu nedenle monoamin oksidaz enzimini inhibe edebilecek ve oksidatif stresi azaltabilecek çift etkili ürünlerin bulunması ayrı bir önem taşımaktadır. Bu çalışmada Türkiye’deki marketlerden satın alınan çeşitli meyve çaylarının; ferrik indirgeyici antioksidan güç, bakır indirgeyici antioksidan kapasite ve 1,1-difenil-1-pikrilhidrazil radikal süpürücü metotları ile antioksidan aktiviteleri saptanmıştır ve toplam fenolik madde miktarları belirlenmiştir. Bu çay infüzyonlarının, MAO enzim inhibisyonları florometrik olarak tayin edilmiştir. Elde edilen tüm sonuçlar poşet yeşil çay ve siyah çay ile kıyaslanmıştır. Elde edilen sonuçlarda, bitki çayı örneklerinin antioksidan ve MAO inhibisyon özelliklerinin içerdikleri toplam fenolik madde miktarı ile ilişkili olduğu gösterilmiştir. Nar içeren çay infüzyonları daha yüksek antioksidan aktivite ve fenolik madde içeriğine sahip olup aynı zamanda da yüksek MAO inhibisyonu göstermektedir. Elde edilen sonuçlar, kuşburnu başta olmak üzere çeşitli çay infüzyonlarında 2-3,5 kat arasında değişen MAO–B seçiciliği bulunduğunu da göstermektedir. Fenolik maddelerin MAO inhibisyonuna sebep olduğu bilgisinden yola çıkılarak yapılan bu çalışmada, çay infüzyonlarının MAO inhibisyon yetenekleri de olduğu ilk kez ortaya konulmuştur.

Anahtar Kelimeler:

CUPRAC, çay infüzyonu, DPPH, FRAP, MAO inhibisyonu, Antioksidan aktivite, MAO inhibisyonu

Determination of Monoamine Oxidase Inhibition and Antioxidant Effects of Various Herbal Teas

The therapeutic properties of fruit teas are due to the polyphenolic flavonoids they contain. Compounds inhibiting the flavoenzyme monoamine oxidase (EC 1.4.3.4, MAO) are known to be used as medicines in treatment of neurodegenerative disorders such as Alzheimer’s and Parkinson’s diseases, and in various neurological disorders. There are many studies about natural compounds with antioxidant properties that prevent such diseases by reducing oxidative stress which plays an important role in neurodegeneration. It is therefore of particular importance to find double-acting products that can both inhibit monoamine oxidase and reduce oxidative stress. In this study, antioxidant activities of various fruit teas purchased from markets in Turkey were determined by ferric-reducing antioxidant power, cupric reducing antioxidant capacity and 1,1-diphenyl-2-picrylhydrazyl methods and their total phenolic contents were revealed. MAO enzyme inhibitions of these tea infusions was determined fluorometrically. The obtained results were compared with green and black tea bag infusions. The results showed that antioxidant and MAO inhibition properties of fruit tea samples were related to their total amount of phenolic substances they contained. Pomegranate containing tea infusions had higher antioxidant activity and phenolic content and exhibited high MAO inhibition. The results also showed that a MAO–B selectivity varying between 2 to 3,5-fold existed in various tea infusions with the highest value in rose hip tea. MAO inhibition abilities of tea infusions were demonstrated for the first time in this present study which was based on the fact that phenolic substances are responsible for MAO inhibition.

Keywords:

CUPRAC, tea infusion, DPPH, FRAP, MAO inhibition, Antioxidant activity, MAO inhibition,

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