Bitki mutantları, bitki ıslahı ve fonksiyonel gen çalışmaları için önemli biyo-kaynaklardır. Mevcut çalışmada, tuza toleranslı aday mutant buğday hatları elde etmek için somatik embriyogenez ile konvansiyonel kimyasal mutajenez tekniği birleştirildi. Bu amaçla; ekmeklik buğdayda (Triticum aestivum L. cv. Adana 99) genetik varyasyonlar yaratmak için embriyonik kalluslara 0-5 mM Sodyum Azid (NaN3), 30 dakika boyunca in vitro ortamda uygulandı. İşlem görmüş ve görmemiş kalluslar, somatik embriyo teşvik ortamına konularak somatik embriyo teşviki için 3 ve 4 mM NaN3 uygulaması optimum mutasyon dozları olarak tespit edildi. Ardından bu mutasyon dozları ile muamele edilen kalluslardan elde edilen somatik embriyolar tuz stresine tolerans geliştirmek için kullanılacak 125 mM NaCl içeren rejenerasyon ortamlarında tolerans açısından tarandı. NaN3 muamelesinde, orta düzeyde tuza toleransı olan 14 mutant elde edildi. Elde edilen sonuçlar; kimyasal mutagenez ile kombine halde in vitro teknik uygulamasının, popülasyonlarda yeterli genetik varyasyon oluşturmak ve 1.5 yıldan daha az bir sürede dördüncü jenerasyon tuz toleranslı aday buğday mutant hatlarını ıslah sürecini hızlandırarak elde etmek için kullanışlı bir yöntem olabileceğini göstermektedir.

OBTAINING CANDIDATE SALT TOLERANT WHEAT MUTANT LINES DERIVED FROM COMBINATION OF SODIUM AZIDE MUTAGENESIS AND SOMATIC EMBRYOGENESIS

Plant mutants are important bio-resources for crop breeding and functional gene studies. In the present study, conventional chemical mutagenesis technique was combined with somatic embryogenesis to obtain candidate salt tolerant mutant wheat lines. For this purpose, 0-5 mM Sodium Azide (NaN3) was applied for 30 minutes to embryonic calli under in vitro conditions to produce genetic variations in the bread wheat (Triticum aestivum L. cv. Adana 99). Treated and non-treated calli were put in somatic embryo induction media, and 3 and 4 mM NaN3 were determined as optimum mutation doses for somatic embryo induction. The obtained somatic embryos from these optimum mutagen doses were then screened for tolerance in regeneration media containing 125 mM NaCl to be used to improve tolerance to salt stress. In NaN3 treatment, 14 mutants with moderate salt tolerance were obtained. The results suggest that the in vitro technique in combination with chemical mutagenesis may be a useful approach for accelerating breeding strategies to create enough genetic variation in populations and to get fourth generation putative salt tolerant wheat mutant lines it less than 1.5 years.

Keywords:

Sodium azide, in vitro mutagenesis, salt tolerance somatic embryogenesis, wheat,

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Trakya University Journal of Natural Sciences-Cover

ISSN: 2147-0294
Yayın Aralığı: Yılda 2 Sayı
Başlangıç: 2000
Yayıncı: Trakya Üniversitesi

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