Water is one of the indispensable and vital elements of living life. It was aimed to investigate the microbial pollution of the tap water that taken from the food enterprises producing. In total, 100 unit of water samples that taken from the food enterprises in the Aegean Region were used as material. Microbiological analyzes of water samples were determined according to the parameters in Annex-2/A "Regulation on Waters for Human Consumption". E. coli between 1-103 cfu/100mL and coliform group between 1-110 cfu/100mL were detected in 10% and 16.25%  of 80 samples of drinking-tap water, C. perfringens (including spores) were not detected in any of the samples. E. coli between 10-62 cfu/100mL, coliform group bacteria between 10-89 cfu/100mL and couting colony at 22 and 37°C between 16-145 cfu/100mL were detected in 20%, 50% and 35% of 20 samples of spring water, C. perfringens (including spores) and P. aeruginosa were not detected in any of the samples. Coliform group microorganisms and E. coli that indicator microorganisms of fecal pollution have detected are above the legal limit in some water in our work. This situation constitutes a risk ethic in terms of public health. That’s why, all hygiene controls must also be thoroughly performed in terms of public health.  Keywords: Drinking-tap water, Hygiene, Microbiological, Public health