Objective. Water is one of the most important elements for human life and an essential part of general nutrition. Water plays crucial roles in proper functioning of body parts, metabolic balance and various biochemical reactions. Adequate daily water consumption is vital for metabolism. The current study was designed to determine the effects of daily water consumption on blood biochemical markers in healthy individuals.Methods. This study was performed 50 healthy volunteers (17 male- 33 female) and aged 21-55 years. Two groups which consuming < 2 liters of water per day and consuming ≥ 2 liters of water per day created based on their interview and answers for the given survey.  Serum fasting blood glucose, total protein, albumin, urea, uric acid, creatinine, sodium, potassium, chlorine, calcium and phosphorus levels were measured in blood samples.Results. The results of this study shows that there is a significant difference of water drinking behavior between male and female where female drink more water than male. Serum urea, uric acid, creatinine and sodium levels increased significantly in the group consuming < 2 liters of water per day than the group consuming ≥ 2 liters of water per day (p<0.05). Conclusion. Water contributes to cell duplication and it also helps formation of urine to clear the body from toxins. In our study, the higher levels of urea, uric acid and creatinine in the group consuming < 2 liters of water per day may be explained by more effective functioning of kidneys and toxin removal in the group consuming ≥ 2 liters of water per day. The current study relates water intake with biochemical parameters in healthy individuals.