Çağlar ÇALLI, Aylin ÇALLI, Ercan PINAR, Semih ÖNCEL, Fırat DEMİRTAŞOĞLU

Skuamöz hücreli larenks kanserinde Ki-67 ve p53 ekspresyonu

Amaç: Bu çalışmada, skuamöz hücreli larenks karsinomlarında p53 tümör baskılayıcı gen ve Ki-67 antigen ekspresyonunun diğer konvansiyonel prognostik parametrelerle ilişkisi değerlendirildi.Hastalar ve Yöntemler: Skuamöz hücreli larenkskarsinomu tanılı 37 hastanın ameliyat örneklerinde Ki-67ve p53 ekspresyonu araştırıldı. İmmünperoksidaztekniği ve monoklonal antikorlar kullanılarak, hastalara ait rutin fikse frozen ve parafine gömülü dokukesitleri immünhistokimyasal analiz yöntemiyle değerlendirildi.Bulgular: Histolojik derecelendirmede tümörler beşolguda iyi, 24 olguda orta, sekiz olguda ise kötü diferansiye olarak belirlendi. On olguda lenf nodu metastazı izlenirken, vasküler invazyon dokuz, sinir invazyonu 15 olguda görüldü. p53 pozitifliği 15 olguda %40.5 , Ki-67 ekspresyonu ise 31 olguda %83.8 saptandı. Ki-67 ekspresyonu lenf nodu metastazıolanlarda daha yüksek oranda saptanırken, ilişki anlamlı bulunmadı. Ki-67 ve p53 ekspresyonu ile konvansiyonel prognostik faktörler arasındaki ilişki anlamlı bulunmadı p>0.05 .Sonuç: Çalışma verileri p53 ve Ki-67’nin skuamözhücreli larenks karsinomlarında prognostik parametrelerle ilişkisi olmadığını göstermektedir

Anahtar Kelimeler:

Karsinom, skuamöz hücreli, gen, p53, baş-boyun neoplazileri, immünhistokimya, Ki-67 antigen, larenks neoplazileri, tümör belirteci, biyolojik

Expression of Ki-67 and p53 in laryngeal squamous celi carcinomas

Objectives: The aim of this study was to investigate the expression of p53 tumor suppressor gene and Ki-67 antigen in laryngeal squamous celi carcinoma and its relation to conventional prognostic indicators.Patients and Methods: p53 and Ki-67 expressions were evaluated in surgical specimens of 37 patients 34 males, 3 females; mean age 57.2 years; range 35 to 75 years with squamous celi carcinoma of the lar- ynx. Immunohistochemical studies were performed in routinely fixed and paraffin-embedded tissue sections using the immunoperoxidase technique and monoclon- al antibodies.Results: The tumors were well-, moderately, and poorly differentiated in 5, 24, and 8 patients, respec- tively. Ten patients had lymph node metastasis, nine patients had vascular invasion, and 15 patients had neural invasion. Ki-67 and p53 nuclear staining was detected in 31 83.8% and 15 40.5% tumors, respectively. The incidence of Ki-67 expression was higher in patients with lymph node metastasis, but this did not exhibit significance. Neither p53 nor Ki-67 immunoexpression was in significant association with conventional clinicopathological parameters p>0.05 .Conclusion: These data indicate that p53 and Ki-67 are not correlated with conventional prognostic para meters in squamous celi carcinoma of the larynx.

Keywords:

Carcinoma, squamous celi, genes, p53, head and neck neoplasms, immunohistochemistry, Ki-67 antigen, laryngeal neoplasms, tumor markers, biological,

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Batsakis JG. Tumors of head and neck: Clinical and pathological considerations. 2nd ed. Baltimore: Williams & Wilkins; 1979. p. 574.
Goldsmith MM, Pillsbury HC. Pathologic and cyto- metric indicators of biologic behaviour in laryngeal cancer. In: Silver CE, editor. Laryngeal cancer. 1st ed. New York: Thieme Medical Publishers; 1991. p. 27-32.
Greenman J, Homer JJ, Stafford ND. Markers in cancer of the larynx and pharynx. Clin Otolaryngol 2000;25:9- 18.
Maestro R, Dolcetti R, Gasparotto D, Doglioni C, Pelucchi S, Barzan L, et al. High frequency of p53 gene alterations associated with protein overexpression in human squamous cell carcinoma of the larynx. Oncogene 1992;7:1159-66.
Levine AJ, Momand J, Finlay CA. The p53 tumour suppressor gene. Nature 1991;351:453-6.
Birrell GW, Ramsay JR. Induction of p53 protein by gamma radiation in lymphocyte lines from breast can- cer and ataxia telangiectasia patients. Br J Cancer 1995;72:1096-101.
Xia F, Wang X, Wang YH, Tsang NM, Yandell DW, Kelsey KT, et al. Altered p53 status correlates with dif- ferences in sensitivity to radiation-induced mutation and apoptosis in two closely related human lym- phoblast lines. Cancer Res 1995;55:12-5.
Söylemezoğlu F, Ayhan A. Tümör prognozunda hücre proliferasyonunun morfolojik ve immunohistokimya- sal olarak değerlendirilmesi. Patoloji Bülteni 1995;12:66-9.
Gerdes J. Ki-67 and other proliferation markers useful for immunohistological diagnostic and prognostic evaluations in human malignancies. Semin Cancer Biol 1990;1:199-206.
Masuda M, Iki M, Takano Y, Asakura T, Noguchi S, Ikeda I, et al. Prognostic significance of Ki-67 labeling index in urothelial tumors of the renal pelvis and ureter. J Urol 1996;155:1877-80; discussion 1880-1.
Lavertu P, Adelstein DJ, Myles J, Secic M. P53 and Ki- 67 as outcome predictors for advanced squamous cell cancers of the head and neck treated with chemora- diotherapy. Laryngoscope 2001;111(11 Pt 1):1878-92.
Lera J, Lara PC, Perez S, Cabrera JL, Santana C. Tumor proliferation, p53 expression, and apoptosis in laryn- geal carcinoma: relation to the results of radiotherapy. Cancer 1998;83:2493-501.
Hirvikoski P, Kumpulainen E, Virtaniemi J, Johansson R, Haapasalo H, Marin S, et al. p53 expression and cell proliferation as prognostic factors in laryngeal squa- mous cell carcinoma. J Clin Oncol 1997;15:3111-20.
Sittel C, Eckel HE, Damm M, von Pritzbuer E, Kvasnicka HM. Ki-67 (MIB1), p53, and Lewis-X (LeuM1) as prognostic factors of recurrence in T1 and T2 laryngeal carcinoma. Laryngoscope 2000;110:1012-7.
Dolcetti R, Doglioni C, Maestro R, Gasparotto D, Barzan L, Pastore A, et al. p53 over-expression is an early event in the development of human squamous- cell carcinoma of the larynx: genetic and prognostic implications. Int J Cancer 1992;52:178-82.
Kazkayasi M, Hucumenoglu S, Siriner GI, Hucumenoglu M. Over-expression of p53 and c-erbB- 2 oncoproteins in laryngeal carcinoma. Eur Arch Otorhinolaryngol 2001;258:329-35.
Nadal A, Campo E, Pinto J, Mallofre C, Palacin A, Arias C, et al. p53 expression in normal, dysplastic, and neo- plastic laryngeal epithelium. Absence of a correlation with prognostic factors. J Pathol 1995;175:181-8.
Klatka J. Prognostic value of the expression of p53 and bcl-2 in patients with laryngeal carcinoma. Eur Arch Otorhinolaryngol 2001;258:537-41.
Salam MA, Crocker J, Morris A. Over-expression of tumour suppressor gene p53 in laryngeal squamous cell carcinomas and its prognostic significance. Clin Otolaryngol 1995; 20:49-52.
Nylander K, Nilsson P, Mehle C, Roos G. p53 muta- tions, protein expression and cell proliferation in squa- mous cell carcinomas of the head and neck. Br J Cancer 1995;71:826-30.
Roland NJ, Caslin AW, Bowie GL, Jones AS. Has the cellular proliferation marker Ki67 any clinical rele- vance in squamous cell carcinoma of the head and neck? Clin Otolaryngol 1994;19:13-8.
Welkoborsky HJ, Hinni M, Dienes HP, Mann WJ. Predicting recurrence and survival in patients with laryngeal cancer by means of DNA cytometry, tumor front grading, and proliferation markers. Ann Otol Rhinol Laryngol 1995;104:503-10.